Cryo- EM structure of large subunit (LSU) of 75S ribosome with A/P- & P/E- tRNAs from Entamoeba histolytica

2025-10-15 REL PDBj INITIAL_RELEASE REPOSITORY PDBj PDBj 2025-05-19 2025-10-15 2025-10-15 2025-10-15 Department of Biotechnology (DBT, India) BT/PR45101/DRUG/134/121/2022 India Cryo- EM structure of large subunit (LSU) of 75S ribosome with A/P- & P/E- tRNAs from Entamoeba histolytica Sharma S Mishra S Gourinath S Kaushal PS Large Subunit of Ribosome, A/P-tRNA, P/E-tRNA, Entamoeba histolytica, Ribosome Sharma S Mishra S Gourinath S Kaushal PS Cryo-EM structure of ribosome from pathogenic protozoa Entamoeba histolytica reveals unique features of its architecture. Nat Commun UK 16 7758 7758 2025 40835601 doi:10.1038/s41467-025-62767-x 2041-1723 9v25 FULLOVERLAP EMDB EMD-64717 associated EM volume

Cryo-EM structure of Ribosome large subunit of 75S ribosome with A/P and P/E-tRNA from Entamoeba histolytica Cryo-EM structure of Ribosome large subunit of 75S ribosome with A/P and P/E-tRNA from Entamoeba histolytica 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 Entamoeba histolytica HM-1:IMSS ribosome cytoplasmic 25S rRNA Entamoeba histolytica HM-1:IMSS 1.130459875 1 AGAUUUGAGUAUCGUAAGAGGACUCCCUUAACUUAAGCAUAUCAAUAAGGGAAGGAAGAAAAAAUAACAAUGAUUUCUUA AGUAAUGGCGAAUGAAAAAGAAAAAGACCAAGUUGAUAAGAAUGGAUAAUUGUAAAAGGGUUAGAAGAAUACAGCAAUGU AUGAGGCUAGUCUUAAAUUAUUCAGAGUGUAACUAAUAGAAAAGCUAUAUUUAAAAGGGAUAAUUUAAAUUGAAUUCAGA ACGAAUAUUGAGCCAGAGAGAGUGAAAGCCUCGUCAAUUUAGAUAUAUCUUGAAAAGAAUAUGGUUUAGAAAAGAGUAAA UUUAUUUGGAAGUAUAAAUCGAAAAGGUGAUAGUAUUCAUCAAAGUCUAAAUAUUACAGGAAAACUGAUAGCAAAGAAGU ACUGUGAAGGAAAACUUAAAAGAACUUUGGAAAAAGAGUGAAAAGAGCUUGAAAGAGUAAUAUGUGAACGGAUAAUUUAU AAGAAAAAUAGUGGAAUAUGUUAUAAAUAAAGAUGUGAAAAGAUUAGAUAUAUAAGUAGUAUAUUUAAUAAGAUUAUGUU GAAAUUUAUAAUAUAAGAAAUUAUUAAGAGCAAGAGAAUUAUUUCAUUUAUAGUGAUUAUUAAUAAACGAGGAUAUUAGG AGAAAUCUUAGUAUUCAAUUGUGAAUUAAAAGUAAAAAUCCGAUAAUAAUGGAAUGAUAAAAGCUAUUUAAGGUAAGAUU AAUAAAUUUAAUCCCGAGAAUAGAAACUCUUGAAGAAGUAAAUUAUCUACCCGUCUUGAAACACGGACCAAGGAGUCUAA CAUGGAUGCGAAUGAGAAUAGCAGGAAAGCUAUAACAUGAAGAGAAAACGAAAAACUAUGAGAGUGUAGAAAUACAUUUA GCAAUAGUAUCUUUCAAUAGAAGAAGUAAAUAUGUUAGGACCCGAAAGAUGGUGAACUAUACUUGAACGGAUUGAAGCCG AAGGAAACUUUGGUGGAAGAUUCACGCAGUGUUAACGUGCAAAUUACUUGUCAUAUUUGAGUAUAGGGGCGAAAGACUCA UCGAACUAUCUAGUAGCUGGUUCCACCUGAAUAUUCCCUUAGGAAGGUCAAAGUAUUAAUAGUAUUGAUUGGUAAAGAUA AUGAUUAGGUGUAAUGGGUGAGUAUAUUCACUCAACAUCUUGUCAAACUAUGAAUAAACAAAGAAGAAUUCUCAGUGUUA AAUACACUUCAGGAAUUUAAAUAUAAACAUACUUUGAAUCGGCCAAGUCUGGUAAGCAGGACUGGCGAUGCGGGAUGAAC CGAACGUCGAGAUAAAGUGCCAAAAAGAAACAUAACGAGUACCACAAAAGGUAUUCAUUCAUAUUGACAAUAGGACGGUG GCCAUGGAAGUCGGAAACCGCUAAGGAAUGUGUAACAACUCACCUAUCGAAUGAAUGAGUCCUGAAAAUGGAUGGCAUUA UAAUUUCUUAACUGAUACUCGACCGUUAAAAUGAAGACAUUUUAACGAGUAAGGAAACGAAAUGGCGUAAGGACAACAAA AGUAGUAAAGAUUUAAAGUAAUUUAGAUUGAAACUGUCCUUAGUGCAGAUCUUGGUGGUAGUAGCAAAUACUAAGAAGAG GAAAAAAACAUCUUAGAACUGAAGAGGAGAAAGGUUCCUAAACAACAUUACUUGGAUUAGGGUGAAUCGAUCCUAAAUUA CAGAUGGAAAUCCUUAAGAAAUAUAUAAGUGACAUAUAGUCAAUAUAAGGAUUGAAAGGGAAUAAGAUUAAUAGUUCUUA AUCAAGAGAAUUAAUGAUUAAAUCUUAGAAAUAAGAUUAAAUCGAAUAAAAUGGUAACAUAUCAUGAACUUUAAAAUGGA GUGUGAAAAUCUACGGAAGAGUUCUCUUUUCUUCUUAACAAAAGUAAAUUCCACUGAAAUGAAUCAAUCAGAGAAGUGGA AUUUAUUGGCUUUGUAAGAAAUUCAACAUAUGAUUGAAUUGUGGUAUUUUUGCAGUCUUCAUAAAAAUUAAAGGGAAUCU UAGCAUAAAGCUAAUUAAAGAAUUCUUUUGCUCGUACUAAUAACCGCAACAGGUCUCCAAGAAGCAAAAUCUUUAGUUAA AUAGAAUAACGCAGGUAAGGGAAGUCGGCAACAUAGCUCUGUAAGUUAGCAAGAAAGAGUAGCUCUGGUGAUUUCCUUGU AAAAGGAUUAAAAUCGAAUCAGAACUUUAAAGGUUAGGGGAAUCCGACUGUUUAAUAAAAACAUAGCAUUCUGAUAAAUC UAAAAGGUUCAUUACAGAAUGUGAUUUCUGCCCAGUGCUCUGAAUGUCAAGGUAAUGAAAUUUACUUAAGCGCGGGUUAA CGGCGGGAGUAACUAUGACUCUCUUAAGGUAGCCAAAUGCCUCGUCAUCUAAUUAGUGACGCGCAUGAAUGGAUCACCGU GAUUCCCACUGUCCCUAUCUGCAAUCUAGUGAAACCACAGUCAAGGGAACGGGCUUGAUUGGAACGGCGGGGAAAGAAGA CCCUGUUGAGCUUGACUCUAAUUUAUCAUGUGAAAUCGAUUUUUUGGUAUAGGCUAGGUGGGAGACUUUGUCAAACGUGU AAUACCACCACUAGAAAUACUAUUUCACUCAUUUCGUUAAAUGUUAAAAUGGAUUUUCCAUUAAUUUAAAAACAUUAAAA CUUCGGUUGAAACAGGCGAUUGAAAGAGAUAGACGGGGAGUUUGGCUGGGGCGGCACAUCUGUUACAAGAUAACGCAGGU GUCCAAAGAUGAACUCAAUGAGGACAGAAACCUCAUGUAGAAUAUAAGGGUAUAAGUUCAUUUGAUUAUUGAUUCCAGUA CAAUCAAUAACUCUGAAAUGAUGGCCUAACGAUCCCUAAGAUUUUUAAUUAGAAAGAAUUUAAAACAUAUUAGAUUUAUC UGAUGUGUGAGACCGUAAGGUGAUUUCUAGUUAAUCCUUUAAUAAUAAGGCUUGGGGUGUCAGAAAAGUUACCACAGGGA UAACUGGCUUGUGGCAGCCAAGCGCUCAUAGCGACGUUGCUUUUUGAUCCUUCGAUGUCGGCUCUUCCUAUCCAUUUCAA GCAGAAUUGAAAAAAGGUAUGAUUGUUCACCAUUUAAUAGGGAACGUGAGCUGGGUUUAGACCGUCGUGAGACAGGUUAG UUUUACCCUACCGUUUCGGUAGAUUUUCAAAGUAUUUAGAUAGUAGCACAACUCAGUACGAGAGGAACCGUUGUGUCACG CCACUGGAUUAUUCAAUUAUUUGAUCAAAUAGUGUUGAGAAUCUAACACGUGUUGAAAUAAUGGCUGAAAGCCUCUCAAG CCAGAAUUUCGUCUAGAUGAAUACUUUGUCUUUUUCUUGUUACUUUAGUACAAUUGAAAAUUAUAUACGUUGUAUUUAUU AUCUCGUGUAUUACUCUAUCAAAAGGGUAGUGAAUACUGAGUUAACAAAAUUUAAAGCAUAACUGUUGCUUAUAGACGAU GGCAUCAGAAAUCGGUGUUGUAAGCUUUAGAGUUUCUUAAUCAAUACUCUCGGGUAUUGAAUG BDEQ01000001.1 5.8S rRNA Entamoeba histolytica HM-1:IMSS 0.049892578 1 AAUAAAAUAUUACUUUGGAUAGUUUAGUUUCCUGGGCGAUGAAGAACGCAAUGAAUUGCGAUAAGUGAUAGGAACAAUAA AAUGUGAAUAUCCAAAAUUUGAAUGCUUGAAAGUAUACUUAUGAACUUCAAGGUAUAUAUGAUAUUCAAUAUCCA X65163.1 5S rRNA Entamoeba histolytica HM-1:IMSS 0.037450051 1 AGGUAGAUUACCUGACGUUCCCAUCUGUGUGAACAUAAUGGUACUGAUCAGGCUCUAAGCAUCUUUACUUCCGUGUUCGG AUGGGAACGGGUGUAUCCUACUUGAUAUGAACCUACC AY843008.1 Large ribosomal subunit protein uL2 Entamoeba histolytica HM-1:IMSS 0.02785002 1 LEVO MGRRTLSQRKGRGSIFKSRSHHKLGVAQHRAIDFFERHSTVRGLVKSIEHDPGRGAPLARVVFRNPRQYGLDKELFICAE GLYSGQYIYCGSKASLHIGNVLPIGQCPEGTVVCNVESKPGDRGIIARASGNYATVISHNADNETTRIRLPSGAKKTLKS NCRAMVGIIAGGGRTEKPILKAGVAYRMYKAKRTTWPRVRGVAMNPVDHPHGGGNHQHVGHPTTLKRSSPPGQKAGKVAA RRTGLIRGGNKEGAADN O15574 60S ribosomal protein L3, putative Entamoeba histolytica HM-1:IMSS 0.04562043 1 LEVO MSHRKFEAPRHGSLAFHPRKRVHKVRATVSAFPKDNAAEKPHLTGFLGFKAGMTHVIREVKRTNTKLPKDGVLEPVTIIE TPPMVVAGFVGYKKTTTGLKPITAVFAEHIADEFKRRYTKKWYKNTKNQFAVHTEKYNDVKAKSKRERQIRLIKNRCDVV RVIAHTQMALVPLKQKKAEVMEIQINGGSIAEKVDFAVSLLEKQISVNSVFGTDECIDVCSVTKGHGYNGVIKRFGVRHL PRKTHRGLRKVACVGAWHPARVSWTVARAGQMGFFKRTEVNKKIYRLGCGDLKNAKTEFDITEKGITPMGGFPHYGVVKN DFLMIKGTVAGIRRRVISLRKACFPSTTRTAQEQIVLKFIDTSSKYGHSRFQTTTEKKARMGPMKKDLERQRAEKVEEGK KL C4LW09 60S ribosomal protein L4, putative Entamoeba histolytica HM-1:IMSS 0.047430116999999994 1 LEVO MSLRPFVTIYDGITGEAEKTPVRLPAVFLAPIRGDVVHFVYRNQSKNTRQPEGVSTEAGKQHSAISWGTGRAVARIPRIS GSGSGRNGQGAFGNMTRKGHMFSPLKNFRKWQRKTPKQMRRFAVASCIAASAVPALVSARGHHIAGVPQIPLVVNSKSIS VIKKTKQAVYLLKKINAYSDVLKVIASKTVRAGQGKMRGRKIKERKGPLVVYGNDDLQAVKAFRNIPGVDTCRVENLSVL NLAPGCHMGRFIIWTESAFKKLNTIFGTQKKMAQGKSGFRIAHAQMAVPDMKRVVVAAEKAKLLRAKIVLPKVPKRANPL KNWAAMVKLNPYAKIASHKLAQVAKAQAAHKAQYAAKMEKILAAKKEALNQSVAKRFGKTQKVDGKVIKVKVENNLLKAT VKRTAKQDAYMKKYDGIVKANAKKYAEKIGF B1N384 60S ribosomal protein L5, putative Entamoeba histolytica HM-1:IMSS 0.032437815999999994 1 LEVO MGFLKVIKNRAYFKKYQTQFRRRREGKTDYYARRKMIFQDKDKFKTPKYRVVVRITNKTVIAQIAYSEIIGDKILCAAYS HELPRYGVKLGLTNYAAAYCTGLLLARRLLQKLGMDKQFVGVSDAAKLGEDYTPEEVDERRPFKCILDVGLARTTTGSKV FAVLKGMCDGGVYVPHSATRFAGAGENEEALRSRILGGHVAAYMKYLSGEDADLYKKQFGRFIAAGIAPEALENIYIEAH KKIRENPAAEKKEKKEHKKYPTSKKLTLEQRKAAIQAKLAKLAQQA C4LU56 Large ribosomal subunit protein eL6 Entamoeba histolytica HM-1:IMSS 0.022958322 1 LEVO MSAPQWFVPVKAGDAKVPAYYPTDDMIQEQKKKLRTRKGAKITALDKPAWNLEKLRKSIVPGAILIIVAGKYAGKKVVFL KQCIKSGALIVTGPFKINGVPLMRINQRYVIATSQKVDVSKVDTTGVDVKFFKKIAIKKAAFGKTVEEDFAKKQYEAKKA LIVEKQKQVDESIVAEIKKVPYLKEYMASYFTLKNGDHPHLLKF O15595 60S ribosomal protein L7, putative Entamoeba histolytica HM-1:IMSS 0.025765818 1 LEVO MSAKVATAVQRETCTKTVCPCKARCQAFRKEVILRTIKNHKDIEAAKKAVYTAKKNAEINGDLYAEADPKLIVAIRIRGI NGVSPKIKKILKLLRLRQINNAVFIKANASTIKMLRLVDPYVTYGYPTLETVQKLIYKRGALKINGNRMPITSNCMIKKA LGDKDVVCVDDLVHEIYTVGKHFKEVNNKLAPFKLNGAKIAEKNKKIHFILGGGFGNRELLINKLLANMI C4M751 60S ribosomal protein L7a Entamoeba histolytica HM-1:IMSS 0.032313945 1 LEVO MSGKSNKQKQAKQTNPLIVANKKVFGKGNVAHPRDLTRYVKYPLYVRIQKEKRLLMKRLKTPPAVNIFANHTLDKTNATQ LFKILDHIKPEERAAKLQRIRAAAKELAEKTQKGEKATASKKTEKPATLSYGINNVVRLIERKQAKLVVIAHDVEPLEMV VYLPYLCKKLQVPYCIVKGKARLGQLIHRSTAAVVAVTEIKKEDKAAFESLVQNVKSIYFENAHMYREFGGRINGFKHNE KQKKIQSKLNKDLADKAKQQAALTVTTAVEEKAAPVAEEKKADKTA C4LVH6 60S ribosomal protein L9, putative Entamoeba histolytica HM-1:IMSS 0.022351931999999998 1 LEVO MPRLLKKTEHVVIPEGVNIEINRKNIKVTGPRGSLTREFQHPKVDIYASKLVVKKEGPKENVVVIDMWYGNRRDSAVVRT IASHIKNMITGVTKGYQYKMRVVYAHFPVTLVIADDGKSIQVQNFLGEKRTRHIEMYDGVVVKKLGKDEICLEGNDVEKV SQSAANIHAANLVRNKDIRQFLDGVYVSEKCLIENDA C4LVZ9 Ribosomal protein L10, putative Entamoeba histolytica HM-1:IMSS 0.023831151999999998 1 LEVO MGRRPGRCYRLVRGHPYPKSKYCRGVPDPRIKLFDIGNRSAPCDDFPCCVHIVGLERENISSEAMEAARISINKNMLKYA GKDGFHVRIRIHPFHVLRINKMLSCAGADRLQTGMRGAWGKSYGSCARVKVGQVLISGRCKEQHLPAMIKSFRLACYKFA GRQKLVISNKWGFTKYTKEEYQQLKKDGKIIADGCYFKLATTKGPLPKVN C4LTA0 60S ribosomal protein L11, putative Entamoeba histolytica HM-1:IMSS 0.019669807 1 LEVO MSACKSDVMRKIKIDKLVLNISVGESGDRLTRAAKVLETLTGQTPVLSEARYTVRAFSIRRNDQIACHVTVRGAKAAEIL EKGLKVKEFELKANNFSATGNFGFGIEEHIDLGLKYDPQVGIYGMDFFVVLARPGFRVSRRKRMQDKIGRDHLLTKEDAI NWFKETYEGIVLNK C4LZG9 60S ribosomal protein L13, putative Entamoeba histolytica HM-1:IMSS 0.033028421999999995 1 LEVO MKHNQAVSNIHFCKDWRSKVHTWFKQPFRKIRRHQTRVEKAKAVFPATIKSLKPSVHCMNQRFNYKLRLGRGFTLKELRA AKIDKNLARTIGIAVDPRRKESSKECLNRNAQRLTEYMNRLVVLPKVHAATAKRLVLNKKNAEAKTKKAAEIKKFIAEHN KTIKELKIKVAAAKKDYAKTKDAAKAKELKACLKGLKKAQVAFAAQVAKEQKKTKQFNKLPVQQKEAKQVLDANKVIRLT APCTLETKTLTKGMKAFDAVAHLRKAKNVAKAVSGIVKGQKKANNAPSKKQ C4M8R8 60S ribosomal protein L13, putative Entamoeba histolytica HM-1:IMSS 0.023177395 1 LEVO MSTNNKVTVIDCHGHVLGRVASVVAKHLLLGQKFVLVRCEDLQVCGTLKMRLVQWELYQRKRVNTNPTRGAFHHRSPADM VRKAVRGMLPKNNYRGKLALKNLKCFEGCPAPYDKMKKSSIPEAAAIYSFNPTRKRTLLGELGTAVGWKYADVVAKNEAE RKERAAKWYAAKQLKQKAVAEAKEKILADEKYKAKVAILKKFGYA C4M3F3 60S ribosomal protein L14, putative Entamoeba histolytica HM-1:IMSS 0.015161095999999999 1 LEVO MVFSRFVEVGRVVLMKTGPFASKLAVIVEILDHNRVLVDGPQAITGVPRQVVNLNTISLTSFVVNKVTRGMTHKKLCLRF TQDAIIKRFNKTATGKAIEKRILREKMTDFDKFQVSIINRKINAAAKIAVSKKAH C4M8C9 Ribosomal protein L15 Entamoeba histolytica HM-1:IMSS 0.023951282999999997 1 LEVO MGAYKYINALNRKKQSDVLRFLRRIRCWELRHLPRIHRVARPTNPERARRLGYKPKSGFVIYSVRIHRGNRKVSARKGIV HRKPACQGIHKIKRVKSLQAIAEQRVGKHCGALRVLNSYFIGQDSTYRFFEVILVDPFNAAIRNDPKMNWICADKQKRRE ARGLTSAAKKSRGFTGKGRRYNKIGAGARGNLKRRNLLQLKRYRS C4M7U2 60S ribosomal protein L17, putative Entamoeba histolytica HM-1:IMSS 0.01989243 1 LEVO MVKYCAKPVNVAKAAQARGDNLKVHFKNTYETADAIRGMKVARAQEFLKNVIIKKEIVPFRKHNGGVGRKAQCKGLNCAF GRWPVKSAQFILKLLKNAVDNAKAKGLNTALLTVSHIAVQEARSSRRRLYRAHGSINSFSSSPCHIELILTEKEKKVAKP TEGIKGKKSTHQKIAYVLN C4LX05 60S ribosomal protein L18, putative Entamoeba histolytica HM-1:IMSS 0.019038791 1 LEVO MAIDLDRHHVRKHVSKTARGNNAYMKLLVRLYGFLARRTQSKFAKTILHRLCLSRVNRPIVSTSKLACLMKKHPEETAVC VNTVTYDSRYPVPKMNVCALKFTKTAEAAITKAGGKCLRFDELALKAPTGRKTVLIRGKRNVREALKHFGKVCAKKNPAK QYKGKQTK C4LXC8 60S ribosomal protein L18a Entamoeba histolytica HM-1:IMSS 0.020130812 1 LEVO MKEYYITARCTDKAHNPNEIFRSRVFAPNYVVAKSKFWMMLKKQHKVKKINAELLQCQPIFEKNPTVVKNYQIFLRYVSA GGIHNITKQYRDVTRVGAVRQMYNDLASQYRTRQDKIQLISITTVGDKAVKNPVLHQFVGHHVKFPMVHCKPVIASKRFN LPIKAGKLTTKLC C4LXZ0 Ribosomal protein L19 Entamoeba histolytica HM-1:IMSS 0.022978787 1 LEVO MVSLKLQKRLAASVLKCGNRKVWLDPNETPEISLANSRKAIRKLIKDRLIIKKPAAMHSRARVVERKQAKILGRHTGHGK RKGTKNARTPQKTLWIIRMRVLRRLLLKYRTTGKIDKHMYHDFYLKVKGNQYKNKKALMESIFKALEEKKLLAKASVQVT KKSTFITEKKPVVAEEPKKVVEKKEEKKSKAGKKNAKK C4LWJ4 60S ribosomal protein L21, putative Entamoeba histolytica HM-1:IMSS 0.018869338 1 LEVO MPLSNGRNRRTRKLFKRPFRGHGLSNTSTILHTYKVGDYVTILCNSSIQHGLPYKAFHGKTGRVWNVNPHAIGVMVNKKV NNRIVVKRLHISPEHIKPSGCQKDFLERKAAVAAIRKQNIQLKKEGKPLLPLPAKRLPKQPRPAELIKGADIKFTTVAPL KFEELY C4LV69 Large ribosomal subunit protein eL22 Entamoeba histolytica HM-1:IMSS 0.015877571 1 LEVO MSKTIKAPAANKVLVEKKKINKTEEKTFSCTIDLTNCTDFIEPKKLVTFFRQTIKVQGRAGNTKGIEVKVADKKVTITTS SAKLCKRYMKYLMKKYLKKNNLREWLRVISDKKDGFELKFFNVQNDEEEVAEETNAQ C4LW40 60S ribosomal protein L23, putative Entamoeba histolytica HM-1:IMSS 0.01526081 1 LEVO MPKRGAGGRQGNKFRVTCGLNNASTVNCADNTGAKTLTIISVKGFHGRLNRLPRAGCGDMVVATCKKGKPEYRKKMHTAV IIRQRRTWRRKDGVTLYFEDNAAVIVNMKGEMKGSAITGPVSKESADLWPKISSNAPTIV C4LVB0 Ribosomal protein L23A, putative Entamoeba histolytica HM-1:IMSS 0.013694311999999998 1 LEVO MAGTKRYTTAFHRPATKNAAKAPKYPRSLKANRNKMDEFSIIKFPLATETALKKIEDHNTLVFLCEQKANKTMIKKAVEK RYGVKVIKVNTLVRLDGLKKAFVRLAPDVEAMEVATKIGLF C4LV64 60S ribosomal protein L24, putative Entamoeba histolytica HM-1:IMSS 0.018649971 1 LEVO MSIKTQVCAFSGFKIPVAKGRKYVRLDLKSFTFINKKSLMQFLRKTNPRKVFWTAIYRQLNKKGQQNELVEKKKTRRVIK VNRGYSGISAEEIAKRKQTQSSEVLAKKRNAAIKEIKEKKAENAAKKEAARKEAKANGKKATNTYKKSNAKLPKAGHANF NSR C4LSA0 60S ribosomal protein L26, putative Entamoeba histolytica HM-1:IMSS 0.023898668 1 LEVO MKFNCKVSSNPSKARKALYTANASQRALMMSARLSKELREKYNVIAIPIHKDDEVKIIKGHQKVAGKVTAVRRSKYVINI DKLTKTKANGQTVPIAIRPSNVIITKLFLNKDREALLAKKAESRKSYKELVAKRNEEVAKAFTAVYPALNMDIFGKKVEP ITEKKPIRIPSKKMAEKLMSKPTLRAYKTGIKVHKAKNAIAAYKAALAKKIAK C4LSU3 60S ribosomal protein L27, putative Entamoeba histolytica HM-1:IMSS 0.015786718999999998 1 LEVO MSKILAPGRVVIILSGRFAGKKAVVSSVNLQGTKDRKYGFVTVVGVERAPLKITRKMSAKVQEMRTSVKSFCKVINVNHV MPTKYTVNLEQFNLIKQVKINNFEAGKVYPISQKKAISSQFAEEYRKGKESWLFTKLRF C4M727 Large ribosomal subunit protein uL15A Entamoeba histolytica HM-1:IMSS 0.017042817999999998 1 LEVO MATRFRQTRRRRGHVSMGYGRIGKHRKQRGGRGNAGGQHHRKTWFTTFHPDYFGKHGMRVFHLKANKYYCPSINVDSLWS LVGKDVQAQYKNAKVGEEVPVIDCVKHGYFKVLGKGFLPKQPVIVRARYFSEKAQQKIKAVGGACELTA Q9BI06 60S ribosomal protein L29 Entamoeba histolytica HM-1:IMSS 0.0073405829999999995 1 LEVO MSKDKNRSSHNQSHKAHRNGFYKPKKSAYMSTKGMNVQVLKNTKAQRKFALAKKLEAKKAANKE C4LUN3 60S ribosomal protein L30, putative Entamoeba histolytica HM-1:IMSS 0.011629423 1 LEVO MSSTKQAKKTQEGVNSKLALVTKSGKYVLGTKATLKTIRDGKSKLVIISSNCPPLERAKIEYYAVLGKVQVVHYSGSNVD LGTACGRYFSVSVLSIIDGGDSDILKASE C4M919 60S ribosomal protein L31, putative Entamoeba histolytica HM-1:IMSS 0.017281588 1 LEVO MAVSKTTNPRVLKYREANKARKEAKKAKAANKKPAKEIKQKEMAPITREMTIHMHAYLHKESFKKRAPKAIKIIRFLAIK TMKTHVVKFDMGLNQFIWSQGIRSVADRIRVRMARLPIEGEEGKFYTLVSYVPVASFKGLVTKTVEEAEN C4LU13 60S ribosomal protein L32, putative Entamoeba histolytica HM-1:IMSS 0.015572593999999999 1 LEVO MVGPVIKPKIVKKYTGHFKRFDSNRFLRVKASWRVPRGIDNRLRRKFKGTNLLPKIGYGSAKATRNMLPCGLYKFPVRNT TDLEMIMMQNRKYAAEISHAVSAKKRQEIIQRAKILNIRVLNADARIKKQEVAQ C4LT96 60S ribosomal protein L34, putative Entamoeba histolytica HM-1:IMSS 0.015481443999999999 1 LEVO MAVRVTYVRKHSYNTKSNGIRIVKTAGGNMTVQHRQKISNGVRCGDCGCVLAGIRHIRPHQYGWLGKSKRTVTRAYGGVK CHKCVQKKILRAFLINEHNLIKLAAAKTNLEKKQSVKEDKKKATKTVKKADKKTQKK C4LYA7 uL29 Entamoeba histolytica HM-1:IMSS 0.013925792999999999 1 LEVO ASLKISAIKAMSKNDMNAKLIELKGELANLRTAKVTGGAPSKISRLRVVKKDIARLLTVMNTQRMEALRKYYKQAKYVPK ELRPKTTRKERRQLTKAQLNLKTVKAAKKAQHFPARKFFVLA 60S ribosomal protein L35a, putative Entamoeba histolytica HM-1:IMSS 0.012143319 1 LEVO MSKCRLHVNAVFMGFQRAQRNQYPNVALLQISGVNTKEDTPFYIGKRVAYVYKVNVKKGSNKPAIRAIFGKIVAPHGNHG VVKARFLHNLPPKAMGQNLKVFLYPSNI B1N4V3 60S ribosomal protein L36, putative Entamoeba histolytica HM-1:IMSS 0.011942094999999998 1 LEVO MARGIAVGLKRGYPVHTMKTAKRHYGVTKRKHVVNDVIREACGFSAYERHMMDLLRRGLDKKALKYAKKHLGTHKRGLAK RDEIQRALEAIKAAHAHLGHHEQH C4LZH1 60S ribosomal protein L37-A, putative Entamoeba histolytica HM-1:IMSS 0.009046786 1 LEVO MTKGTSCRGTRHNKTHILCKRCGKRSWHLQKQRCASCGYPDAKMRQYAWGYKALRRRTQGTGRMRHLKIALKRTTKA C4M0X0 60S ribosomal protein L37a, putative Entamoeba histolytica HM-1:IMSS 0.010321275 1 LEVO MARRTKKVGIVGKYGTRYGASLRKVIKKIEIAQHSKYVCPFCCKEAVRRTACGIWECKGCKKQIAGGAYTLSTISGATVR STVRRLRDAKNSQ A0A175JXH6 60S ribosomal protein L38 putative Entamoeba histolytica HM-1:IMSS 0.008795553999999999 1 LEVO MPKQVQEFKQILKLLKSGSIRAVRVKTNGENTKFKVRCAKYLYTYICADKAKAEELKKAIPKDIHVVEIAKKAPKAE A0A175JTS8 Ribosomal protein L39, putative Entamoeba histolytica HM-1:IMSS 0.006282477 1 LEVO MTGHKGFSYKLKLGKKIHQNRGVPQWFRMKTGNTINYNFKRRHWNRRKMGM C4LTA2 60S ribosomal protein L40, putative Entamoeba histolytica HM-1:IMSS 0.006482917 1 LEVO MGIEPALEAIARKYNCDKMICRRCYARLPLRATTCRKKKCGHSGDLRPKKKIQSKK C4LV09 60S ribosomal protein L44, putative Entamoeba histolytica HM-1:IMSS 0.011603004 1 LEVO MVVVPKTRKTYCPRCDKHTPHKVSQYKKGKDSLHVQGKRRYDRKQQGFGGQTKPVFKKKAKTTKKVVLKIECTKCKYKLQ KCLKRCKHFELGNEKKDK C4LS99 Unknown peptide Entamoeba histolytica HM-1:IMSS 0.000528644 1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) A/P- tRNA Entamoeba histolytica HM-1:IMSS 0.024483572 1 GGCCCCAUAGCUCAGGGAUGAGAGCACGCGACUCUUAAUCGCGUGGUCGCAGGUUCAAGUCCUGCUGGGGCCACCA P/E- tRNA Entamoeba histolytica HM-1:IMSS 0.024617574 1 CUCUUCAUGGUGCAGCCCGUUAGCUCGUCGGGCUUUUAACCCGAAGGUCUUCGGUUCAAAUCCGGCCGAAGAAACCA singleParticle particle 1 7.4

20nM HEPES-sodium salt buffer pH 7.4, 100mM Potassium acetate, 10mM Magnesium acetate, 10mm Ammonium acetate, 3mM DTT

Quantifoil R1.2/1.3 GOLD 300 CARBON HOLEY GLOW DISCHARGE 50 ETHANE 100 290 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.8 3.0 75000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN EPU FEI FALCON III (4k x 4k) INTEGRATING 11840 2.0 1.106 1 142247 CTFFIND PHASE FLIPPING AND AMPLITUDE CORRECTION EMD-2660 1 3.0 FSC 0.143 CUT-OFF RELION 3.1.4 39958 MAXIMUM LIKELIHOOD RELION 3.1.4 MAXIMUM LIKELIHOOD RELION 3.1.4 4 RELION 3.1.4 emd_64717.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 350 350 350 0 0 0 350 350 350 374.50003 374.50003 374.50003 90.0 90.0 90.0 Z Y X -5.030633 16.259207 0.000000000001724 1.0 1.07 1.07 1.07 2.8 AUTHOR ::::EMDATABANK.org::::EMD-64717:::: Other other

the coordinates of 75S with P-tRNA was used

Other other

the coordinates of 75S with P-tRNA was used

FLEXIBLE FIT Coot

phenix.real_space_refinement

REAL 48.600000000000001 emd_64717_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 350 350 350 0 0 0 350 350 350 374.50003 374.50003 374.50003 90.0 90.0 90.0 X Y Z -0.04320153 0.19986446 0.0040488974 0.019776605 1.07 1.07 1.07 AUTHOR ::::EMDATABANK.org::::EMD-64717:::: emd_64717_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 350 350 350 0 0 0 350 350 350 374.50003 374.50003 374.50003 90.0 90.0 90.0 X Y Z -0.041909408 0.2078247 0.0040885354 0.019788934 1.07 1.07 1.07 AUTHOR ::::EMDATABANK.org::::EMD-64717::::