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        <current_status>
            <date>2026-03-18</date>
            <code>REL</code>
            <processing_site>PDBc</processing_site>
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        </revision_history>
        <sites>
            <deposition>PDBj</deposition>
            <last_processing>PDBc</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-05-21</deposition>
            <header_release>2026-03-18</header_release>
            <map_release>2026-03-18</map_release>
            <update>2026-03-18</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China (NSFC)</funding_body>
                <code>32361163669</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China (NSFC)</funding_body>
                <code>32170189</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China (NSFC)</funding_body>
                <code>32241021</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Other government</funding_body>
                <code>2023B1212060050</code>
            </grant_reference>
            <grant_reference>
                <funding_body>Other government</funding_body>
                <code>2023B1212120009</code>
            </grant_reference>
            <grant_reference>
                <funding_body>Other government</funding_body>
                <code>2023M743512</code>
            </grant_reference>
            <grant_reference>
                <funding_body>Other government</funding_body>
                <code>GZC20232691</code>
            </grant_reference>
            <grant_reference>
                <funding_body>Other government</funding_body>
                <code>2023A1515110923</code>
            </grant_reference>
        </grant_support>
        <title>Calypso/Asx/NCP-ub complex</title>
        <authors_list>
            <author>Wang C</author>
            <author>He J</author>
        </authors_list>
        <keywords>PR-DUB, Calypso, Polycomb, TRANSCRIPTION/DNA, TRANSCRIPTION-DNA complex</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Wang C</author>
                    <author order="2">Sun F</author>
                    <author order="3">Zhao H</author>
                    <author order="4">Zhang N</author>
                    <author order="5">Guan J</author>
                    <author order="6">Zhou Y</author>
                    <author order="7">Shuai W</author>
                    <author order="8">Zheng H</author>
                    <author order="9">He J</author>
                    <title>Structural basis of nucleosome deubiquitination by the bidentate Calypso/Asx complex.</title>
                    <journal_abbreviation>Iscience</journal_abbreviation>
                    <country>US</country>
                    <volume>29</volume>
                    <first_page>114958</first_page>
                    <last_page>114958</last_page>
                    <year>2026</year>
                    <external_references type="PUBMED">41782825</external_references>
                    <external_references type="DOI">doi:10.1016/j.isci.2026.114958</external_references>
                    <external_references type="ISSN">2589-0042</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>9v33</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-64748</accession_id>
                <content_type>associated EM volume</content_type>
                <details>Calypso/ASX/Ncp complex</details>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Ternary complex of Calypso/Asx/NCP-ub</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Ternary complex of Calypso/Asx/NCP-ub</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>7</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>8</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>9</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="7227">Drosophila melanogaster</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Histone H3</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.015435125999999999</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFK
TDLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA</string>
                    <external_references type="UNIPROTKB">A0A310TTQ1</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Histone H4</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.011394426</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRK
TVTAMDVVYALKRQGRTLYGFGG</string>
                    <external_references type="UNIPROTKB">P62799</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Histone H2A</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.014297685</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MGMSGRGKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTR
IIPRHLQLAVRNDEELNKLLGRVTIAQGGVLPNIQSVLLPKKTESSKSAKSK</string>
                    <external_references type="UNIPROTKB">Q6AZJ8</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="4">
                <name>Histone H2B</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.013655948</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MAKSAPAPKKGSKKAVTKTQKKDGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAH
YNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK</string>
                    <external_references type="UNIPROTKB">A0A8J0U496</external_references>
                </sequence>
            </protein_or_peptide>
            <dna macromolecule_id="5">
                <name>DNA (147-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="562">Escherichia coli</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.045178797</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <sequence>
                    <string>(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)
(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)
(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)
(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)
(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)
(DG)(DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)
(DG)(DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)
(DA)(DT)(DC)(DG)(DA)(DT)(DA)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
            <dna macromolecule_id="6">
                <name>DNA (147-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="562">Escherichia coli</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.045570019999999996</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <sequence>
                    <string>(DT)(DA)(DT)(DC)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)
(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)
(DG)(DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA)
(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)
(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)
(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)
(DA)(DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)
(DT)(DC)(DT)(DC)(DC)(DA)(DG)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
            <protein_or_peptide macromolecule_id="7">
                <name>Ubiquitin carboxyl-terminal hydrolase calypso</name>
                <natural_source database="NCBI">
                    <organism ncbi="7227">Drosophila melanogaster</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.055928808999999996</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="7111">Trichoplusia ni</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MWSHPQFEKGGGSGGGSGGGSWSHPQFEKGSSENLYFQGEFMNAAGGGSGAQAAAVAAGNNSLSHNALLSTASGATTMPM
AQLADGWLELESDPGLFTLLLKDFGCHDVQVEEVYDLQKPIESPYGFIFLFRWIEERRARRKIVETTAEIFVKDEEAISS
IFFAQQVVPNSCATHALLSVLLNCNENNLQLGDTLSRLKTHTKGMSPENKGLAIGNTPELACAHNSHAMPQARRRLERTG
AGVSSCRFTGEAFHFVSFVPINGQLFELDGLKPYPMNHGGWEDSEDWTDKFRRVMAERLGIATGEQDIRFNLMAVVPDRR
IAITHKLKMLRTNQAIVSGTLQKLLKADEQGESGNGDSQRPDTPTTLLEPSAFTARDLQSLLKNLDTEIAINEQHLADEN
DRRHMFKVDASRRTHNYDKFICTFLSMLAHQGVLGELVSQHLLPSKKVSGQGAANRISKQSTTASAGGSTAAGTASTPKT
QQQQAAAAKNGKSPSKTPGRRRKGRNKCRKRK</string>
                    <external_references type="UNIPROTKB">Q7K5N4</external_references>
                </sequence>
                <ec_number>3.4.19.12</ec_number>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="8">
                <name>Polycomb group protein Asx</name>
                <natural_source database="NCBI">
                    <organism ncbi="7227">Drosophila melanogaster</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.037097965</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="7111">Trichoplusia ni</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MKTITPDTTTTTSSQHQQLLIPQADQHHQPMLQQQSLLAAPPPTMIMEHVNLVDDDEKDPLALEQLEVSPSTKHTHSLRR
HLPRIIVKPIPPEKKPMAPSEEAAVSTAPAPPTRLICSRRIQQQQQVKAAAAAAAAAAAAAAAAAAAAQAQATSSYPSAI
SPGSKAGTSQASTMREVLASIPGFSVKPRRRSNKKLTTAAQIEQTKDGKIDLETPDSILASTNLRALLNKQTFSLLPPLY
QYNLIQLLPSVDREASELEQPSSSASGGSPSEAIRLSASCLNNEFFARACLEWRERLSEGEFTPENQLKLKTEAEREKNK
LDPWKLKHFEPFWGEKNSRG</string>
                    <external_references type="UNIPROTKB">Q9V727</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="9">
                <name>Polyubiquitin-B</name>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.010001402</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>GPHHHHHHLGSHMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLH
LVLRLRGC</string>
                    <external_references type="UNIPROTKB">P0CG47</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
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                        <ph>7.5</ph>
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                        <cryogen_name>NITROGEN</cryogen_name>
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            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">0.8</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.4</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON IV (4k x 4k)</film_or_detector_model>
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                        </image_recording>
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                    <pdb_model>
                        <pdb_id>6CGA</pdb_id>
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                    <resolution units="Å" res_type="BY AUTHOR">5.9</resolution>
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                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>208</col>
                    <row>208</row>
                    <sec>208</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>208</x>
                    <y>208</y>
                    <z>208</z>
                </spacing>
                <cell>
                    <a units="Å">295.36</a>
                    <b units="Å">295.36</b>
                    <c units="Å">295.36</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.13832068</minimum>
                    <maximum>1.9074334</maximum>
                    <average>0.04108172</average>
                    <std>0.047066983</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.42</x>
                    <y units="Å">1.42</y>
                    <z units="Å">1.42</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-64748::::</label>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
