2025-11-26 REL PDBc INITIAL_RELEASE REPOSITORY PDBj PDBc 2025-05-24 2025-11-26 2025-11-26 2025-11-26 National Natural Science Foundation of China (NSFC) 32325008 China Cryo-EM structure of A. thaliana MET1 with bound SAH Zhang Z Liu Y Li W Du J DNA methylation, covalent AtMET1-DNA complex, Cryo-EM structure, epigenetic regulation, GENE REGULATION Zhang Z Li W Liu Y Chi C Nan J Wang C Zhu Y Zhao J Xue Y Li Y Wang P Zhai J Du J Structural insights into plant DNA CG methylation maintenance by MET1. Plant Cell US 37 2025 41082561 doi:10.1093/plcell/koaf244 1532-298X 2109 PLCEEW 9v4o FULLOVERLAP EMDB EMD-64781 associated EM volume
Cryo-EM structure of A. thaliana MET1 with bound SAH
 DNA (cytosine-5)-methyltransferase 1 DNA (cytosine-5)-methyltransferase 1 0 1 Arabidopsis thaliana DNA (cytosine-5)-methyltransferase 1 Arabidopsis thaliana 0.177588062
tag(1-40)+DNA (cytosine-5)-methyltransferase 1(41-1574)
1 Baculovirus expression vector pFastBac1-HM LEVO MSYYHHHHHHHHHHDYDIDYKDDDDKPTTENLYFQGAMGSMVENGAKAAKRKKRPLPEIQEVEDVPRTRRPRRAAACTSF KEKSIRVCEKSATIEVKKQQIVEEEFLALRLTALETDVEDRPTRRLNDFVLFDSDGVPQPLEMLEIHDIFVSGAILPSDV CTDKEKEKGVRCTSFGRVEHWSISGYEDGSPVIWISTELADYDCRKPAASYRKVYDYFYEKARASVAVYKKLSKSSGGDP DIGLEELLAAVVRSMSSGSKYFSSGAAIIDFVISQGDFIYNQLAGLDETAKKHESSYVEIPVLVALREKSSKIDKPLQRE RNPSNGVRIKEVSQVAESEALTSDQLVDGTDDDRRYAILLQDEENRKSMQQPRKNSSSGSASNMFYIKINEDEIANDYPL PSYYKTSEEETDELILYDASYEVQSEHLPHRMLHNWALYNSDLRFISLELLPMKQCDDIDVNIFGSGVVTDDNGSWISLN DPDSGSQSHDPDGMCIFLSQIKEWMIEFGSDDIISISIRTDVAWYRLGKPSKLYAPWWKPVLKTARVGISILTFLRVESR VARLSFADVTKRLSGLQANDKAYISSDPLAVERYLVVHGQIILQLFAVYPDDNVKRCPFVVGLASKLEDRHHTKWIIKKK KISLKELNLNPRAGMAPVASKRKAMQATTTRLVNRIWGEFYSNYSPEDPLQATAAENGEDEVEEEGGNGEEEVEEEGENG LTEDTVPEPVEVQKPHTPKKIRGSSGKREIKWDGESLGKTSAGEPLYQQALVGGEMVAVGGAVTLEVDDPDEMPAIYFVE YMFESTDHCKMLHGRFLQRGSMTVLGNAANERELFLTNECMTTQLKDIKGVASFEIRSRPWGHQYRKKNITADKLDWARA LERKVKDLPTEYYCKSLYSPERGGFFSLPLSDIGRSSGFCTSCKIREDEEKRSTIKLNVSKTGFFINGIEYSVEDFVYVN PDSIGGLKEGSKTSFKSGRNIGLRAYVVCQLLEIVPKESRKADLGSFDVKVRRFYRPEDVSAEKAYASDIQELYFSQDTV VLPPGALEGKCEVRKKSDMPLSREYPISDHIFFCDLFFDTSKGSLKQLPANMKPKFSTIKDDTLLRKKKGKGVESEIESE IVKPVEPPKEIRLATLDIFAGCGGLSHGLKKAGVSDAKWAIEYEEPAGQAFKQNHPESTVFVDNCNVILRAIMEKGGDQD DCVSTTEANELAAKLTEEQKSTLPLPGQVDFINGGPPCQGFSGMNRFNQSSWSKVQCEMILAFLSFADYFRPRYFLLENV RTFVSFNKGQTFQLTLASLLEMGYQVRFGILEAGAYGVSQSRKRAFIWAAAPEEVLPEWPEPMHVFGVPKLKISLSQGLH YAAVRSTALGAPFRPITVRDTIGDLPSVENGDSRTNKEYKEVAVSWFQKEIRGNTIALTDHICKAMNELNLIRCKLIPTR PGADWHDLPKRKVTLSDGRVEEMIPFCLPNTAERHNGWKGLYGRLDWQGNFPTSVTDPQPMGKVGMCFHPEQHRILTVRE CARSQGFPDSYEFAGNINHKHRQIGNAVPPPLAFALGRKLKEALHLKKSPQHQP P34881 2.1.1.37 S-ADENOSYL-L-HOMOCYSTEINE 0.000384411 1 SAH ZINC ION 6.5409e-05 1 ZN singleParticle particle 7.5 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.5 GATAN K3 BIOQUANTUM (6k x 4k) 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.92 FSC 0.143 CUT-OFF 629218 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_64781.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 272.0 272.0 272.0 90.0 90.0 90.0 X Y Z -0.18259723 19.216439999999999 -0.051940806 0.4590429 0.85 0.85 0.85 2.1 AUTHOR map of AtMET1 with bound SAH emd_64781_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 272.0 272.0 272.0 90.0 90.0 90.0 X Y Z -0.35462296 0.5461915 0.000008355872 0.040260926 0.85 0.85 0.85 AUTHOR Half B map of AtMET1 with bound SAH emd_64781_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 272.0 272.0 272.0 90.0 90.0 90.0 X Y Z -0.33082798 0.54721975 0.000005961232 0.03964982 0.85 0.85 0.85 AUTHOR Half A map of AtMET1 with bound SAH