Binding site between C-reactive protein and c2cc monoclonal antibody

2026-03-18 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _citation_author.identifier_ORCID _em_admin.last_update PDBj PDBj 2025-06-05 2025-11-19 2025-11-19 2026-03-18 Not funded Binding site between C-reactive protein and c2cc monoclonal antibody Moiseenko AV Kalikin AV c-reactive protein, CRP, monoclonal antibody, immune complex, IgG, IMMUNE SYSTEM Moiseenko AV Kalikin AV Orekhov PS Byzova NA Zherdev AV Shaitan KV Dzantiev BB Sokolova OS Cryo-EM structure of pentameric C-reactive protein in complex with monoclonal IgG antibodies. Febs J. UK 293 1376 1385 2026 41159871 doi:10.1111/febs.70310 1742-464X 9vca FULLOVERLAP EMDB EMD-64950 associated EM volume

Asymmetric unit of D5-symmetric complex composed of two pentameric C-reactive proteins interlinked with five monoclonal IgG antibodies Asymmetric unit of D5-symmetric complex composed of two pentameric C-reactive proteins interlinked with five monoclonal IgG antibodies 0 1 2 3 Human C-reactive protein 1 1 Homo sapiens Monoclonal antibody 1 2 3 Mus musculus albula C-reactive protein Homo sapiens 0.023068039 1 Homo sapiens LEVO QTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGS EILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVN MWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWP P02741 Monoclonal IgG antibody heavy chain fragment Mus musculus albula 0.049078093999999996 1 Mus musculus albula LEVO QVQLQQSGTELSKPGTGVKMSCKASGYTFISYWMHWVKQRPGQGLEWIGYINPSTDSTDYNQNFKDKATMTVDKSSTTAY MQLSTITSEDSAVYFCARSHYLSWDFDVWGAGTTVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTW NSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFP PKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVN SAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSY FVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK Monoclonal IgG antibody light chain fragment Mus musculus albula 0.023957444999999997 1 Mus musculus albula LEVO DIVLTQSPASLAVSLGQRATISCRASQSVSTSGYSYIHWYQQKPGHPPKLLIKYASNLDSGVPARFSGSGSGTDFTLNIH PVEEEDAATYFCQHSWELPLTFGAGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQ NGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC singleParticle particle 1 7.6 20.0 Tris-HCl 150.0 NaCl

20 mM Tris-HCl buffer (pH 7.6), containing 150 mM NaCl

ETHANE FEI VITROBOT MARK IV

The specimen contains the both interacting compounds, C-reactive protein and monoclonal IgG antibodies in final concentrations 1 mg/mL. To reach immune complexes formation, equal volumes of C-reactive protein and monoclonal IgG antibodies solutions with concentrations of 2 mg/mL each were mixed and incubated for 1 hour at room temperature.

TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.0 2.0 GATAN K3 BIOQUANTUM (6k x 4k) 54.0 1 286000 cryoSPARC 4.6 Patch CTF estimation PHASE FLIPPING AND AMPLITUDE CORRECTION C1 2.4 FSC 0.143 CUT-OFF cryoSPARC 4.6

The resolution estimated within a soft mask covering one pCRP chain and one Fab fragment. The number of particles provided after the D5 symmetry expansion

1434575 MAXIMUM LIKELIHOOD cryoSPARC 4.6 Ab-initio without symmetry MAXIMUM LIKELIHOOD cryoSPARC 4.6

Local refinement made with the mask covering one chain of pCRP and one Fab fragment. The local refinement was performed with symmetry expanded set of particles, prealigned to a C5-symmetric consensus reconstruction of the whole complex.

emd_64950.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 0 0 0 450 450 450 373.5 373.5 373.5 90.0 90.0 90.0 X Y Z -14.553406000000001 21.325195000000001 0.0009921428 0.043492828 0.83 0.83 0.83 0.285 AUTHOR ::::EMDATABANK.org::::EMD-64950:::: 3HC3 PDB experimental model

IgG antibody as a reference model for sequence replacement

7PKE PDB experimental model FLEXIBLE FIT REAL emd_64950_msk_1.map emd_64950_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 0 0 0 450 450 450 373.5 373.5 373.5 90.0 90.0 90.0 X Y Z -2.195381 4.236432 0.0005185654 0.027308237 0.83 0.83 0.83 AUTHOR ::::EMDATABANK.org::::EMD-64950:::: emd_64950_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 0 0 0 450 450 450 373.5 373.5 373.5 90.0 90.0 90.0 X Y Z -2.5909305 4.374485 0.0005210471 0.027344769 0.83 0.83 0.83 AUTHOR ::::EMDATABANK.org::::EMD-64950::::