cryoEM structure of retron-Eco7 complex (form II)

2026-01-07 REL PDBc INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBc 2025-06-17 2026-01-07 2026-01-07 2026-01-07 National Natural Science Foundation of China (NSFC) China cryoEM structure of retron-Eco7 complex (form II) Dai ZK Wang YJ Guan ZY Zou TT retron-Eco7, toxin-antitoxin, DNA BINDING PROTEIN/RNA/DNA, DNA BINDING PROTEIN-RNA-DNA complex Dai Z Liu C Wang Y Chen X Fu X Yang K Zhu R Jia X Chen Y Tao P Guan Z Zou T Phage nuclease-mediated defense activation of the bacterial Retron-Eco7 toxin-antitoxin system. Nucleic Acids Res. UK 53 2025 41277685 doi:10.1093/nar/gkaf1173 1362-4962 0389 NARHAD 9vhl FULLOVERLAP EMDB EMD-65070 associated EM volume

retron-Eco7 complex retron-Eco7 complex 0 1 2 3 4 5 Escherichia coli Retron Ec78 reverse transcriptase Escherichia coli 0.035629242 1 Escherichia coli BL21(DE3) LEVO MSVIRRLAAVLRQSDSGISAFLVTAPRKYKVYKIPKRTTGFRVIAQPAKGLKDIQRAFVQLYNFPVHDASMAYMKGKGIR DNAAAHAGNQYLLKADLEDFFNSITPAIFWRCIEMSSALTPQFEPQDKFFIEKILFWQPIKHRKTKLILSVGAPSSPVIS NFCMYEFDNRIHAACNKLEITYTRYADDLTFSCNIPNVLKAVPSTIEALLKDLFGSELRLNHSKTVFSSKAHNRHVTGVT INNEETLSLGRDRKRFIKHLINQYKYGLLDNEDKAYLTGLLAFASHIEPGFITRMNEKYSLELMERLRGQR Q46666 2.7.7.49 Retron Ec78 probable ATPase Escherichia coli 0.062466625 4 Escherichia coli BL21(DE3) LEVO MTKQYERKAKGGNLLSAFELYQRNTDNMPGLGEMLVDEWFETCRDYIQDGHVDESGTFRPDNAFYLRRLTLKDFRRFSLL EIKFEEDLTVIIGNNGKGKTSILYAIAKTLSWFVANILKEGGSGQRLSELTDIKNDAENRYADVSSTFFFGKGLKSVPIR LSRSALGTAERRDSEVKPARDLADIWRVINEAKTINLPTFALYNVERSQPFNRNTKDNAGRREERFDAYSQALGGAGRFD HFVEWYIYLHKRTISDISSSIKELEQQVNDLQRSVDGGMVSVKSLLEQMKLKLSEASERNDAAVSSKMVTESVQKSIVEK SICSVVPSISKIWVEMTTGSDLVKVTNDGHDVTIDQLSDGQRVFLSLVADLARRMVMLNPLLENPLEGRGIVLIDEIELH LHPKWQQEVILNLRSVFPNIQFIITTHSPIVLSTIEKRCIREFDPNDDGNQSFLDSPDMQTKGSENAQILEQVMNVHPTP PGIAESHWLGDFELLLLDNSGELDNQSQELYDKIKTHFGIDSAELKKADSLIRINKMKNKINKIRAEKGK P0DV91 Retron Ec78 putative HNH endonuclease Escherichia coli 0.024816848 1 Escherichia coli BL21(DE3) LEVO MKELARLESPEILDQYTAGQNDWMEIDQSAVWPKLTEMQGEFCAYCECRLNRRHIEHFRPRGKFPALTFIWSNLFGSCGD SKKSGGWSRCGIYKDNGAGAYNADDLIKPDEENPDDYLLFLTTGEVVPAIGLTGRALKKAQETIRVFNLNGDIKLLGSRR TAVQAIMPNVEYLYSLLEEFEEDDWNEMLRDELEKIESDEFKTALKHAWTSNQEFA P0DV92 msdDNA Escherichia coli 0.022869648 1 (DG)(DG)(DA)(DA)(DG)(DC)(DG)(DA)(DA)(DA)(DG)(DT)(DG)(DT)(DC)(DG)(DC)(DA)(DA)(DC) (DC)(DC)(DG)(DA)(DG)(DA)(DG)(DA)(DG)(DG)(DA)(DA)(DC)(DG)(DA)(DT)(DC)(DT)(DC)(DG) (DG)(DG)(DT)(DT)(DG)(DC)(DG)(DA)(DC)(DA)(DC)(DT)(DT)(DT)(DC)(DG)(DC)(DA)(DA)(DC) (DC)(DC)(DT)(DT)(DA)(DA)(DT)(DA)(DC)(DG)(DT)(DT)(DC)(DA) U02551.1 DNA msrRNA Escherichia coli 0.020813266 1 ACUCUUUAGCGUUGGACGGUUACGUCUAGUCGGGUGAUUAGCCAGACUCUAACUUAUUGAACGUA U02551.1 ADENOSINE-5'-TRIPHOSPHATE 0.000507181 3 ATP MAGNESIUM ION 2.4305e-05 1 MG singleParticle particle 8.0 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.2 1.6 GATAN K3 BIOQUANTUM (6k x 4k) 50.0 1 NONE 2.6 FSC 0.143 CUT-OFF cryoSPARC 291980 MAXIMUM LIKELIHOOD PROJECTION MATCHING emd_65070.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 263.68 263.68 263.68 90.0 90.0 90.0 X Y Z -2.439853 3.557686 -0.00048476027 0.06731124 0.824 0.824 0.824 0.3 AUTHOR ::::EMDATABANK.org::::EMD-65070:::: emd_65070_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 263.68 263.68 263.68 90.0 90.0 90.0 X Y Z -0.46757045 1.1456838 0.00091575884 0.044701096 0.824 0.824 0.824 AUTHOR ::::EMDATABANK.org::::EMD-65070:::: emd_65070_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 263.68 263.68 263.68 90.0 90.0 90.0 X Y Z -0.49904108 1.2216693 0.0009461543 0.04451688 0.824 0.824 0.824 AUTHOR ::::EMDATABANK.org::::EMD-65070::::