2025-07-23 REL PDBj INITIAL_RELEASE REPOSITORY PDBj PDBj 2025-06-18 2025-07-23 2025-07-23 2025-07-23 Japan Society for the Promotion of Science (JSPS) Japan cryo-EM structure of alligator haemoglobin in the T-like conformation Takahashi K Lee Y Nishizawa T Tame JRH haemoglobin, OXYGEN BINDING Takahashi K Lee Y Nishizawa T Tame JRH Conformational analysis of liganded human hemoglobin by cryo electron microscopy Biorxiv US 2025 doi:10.1101/2025.07.07.661630 2692-8205 9vie FULLOVERLAP alligator haemoglobin in carboxy form alligator haemoglobin in carboxy form 0 1 2 Alligator mississippiensis 0.1 Hemoglobin subunit alpha Alligator mississippiensis 0.015891368 1 LEVO MVLSMEDKSNVKAIWGKASGHLEEYGAEALERMFCAYPQTKIYFPHFDMSHNSAQIRAHGKKVFSALHEAVNHIDDLPGA LCRLSELHAHSLRVDPVNFKFLAHCVLVVFAIHHPSALSPEIHASLDKFLCAVSAVLTSKYR P01999 Hemoglobin subunit beta Alligator mississippiensis 0.016641213 1 LEVO ASFDAHERKFIVDLWAKVDVAQCGADALSRMLIVYPWKRRYFEHFGKMCNAHDILHNSKVQEHGKKVLASFGEAVKHLDN IKGHFANLSKLHCEKFHVDPENFKLLGDIIIIVLAAHHPEDFSVECHAAFQKLVRQVAAALAAEYH P02130 PROTOPORPHYRIN IX CONTAINING FE 0.000616487 2 HEM CARBON MONOXIDE 2.801e-05 2 CMO singleParticle particle 8.0 Quantifoil CARBON HOLEY ETHANE FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.4 1.2 165000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 15 GATAN K3 BIOQUANTUM (6k x 4k) 60.0 1 cryoSPARC 4.6.2 PHASE FLIPPING AND AMPLITUDE CORRECTION 8WIX C2 2.38 FSC 0.143 CUT-OFF cryoSPARC 4.6.2 238763 MAXIMUM LIKELIHOOD cryoSPARC 4.6.2 MAXIMUM LIKELIHOOD cryoSPARC 4.6.2 emd_65085.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 261.12 261.12 261.12 90.0 90.0 90.0 X Y Z -2.1414192 3.3190079 -0.000005115495 0.056780048 1.02 1.02 1.02 0.5 AUTHOR 8WIX PDB experimental model emd_65085_msk_1.map emd_65085_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 261.12 261.12 261.12 90.0 90.0 90.0 X Y Z -0.3322098 0.86138064 0.00016643958 0.016836131 1.02 1.02 1.02 AUTHOR emd_65085_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 261.12 261.12 261.12 90.0 90.0 90.0 X Y Z -0.2995974 0.86288196 0.00015909079 0.016816704 1.02 1.02 1.02 AUTHOR