2025-12-03 REL PDBc INITIAL_RELEASE REPOSITORY PDBj PDBc 2025-06-18 2025-12-03 2025-12-03 2025-12-03 Not funded Cryo-EM Structure of NPFFR1 in complex with peptide NPFF Na M Xu F GPCR, NPFFR1, MEMBRANE PROTEIN Na M Yue Y Xu K Xu Z Zhang L Zhou F De Neve J Ballet S Xu F Molecular Recognition at the Opioid-modulating Neuropeptide FF Receptor 1. Protein Cell CN 2025 41206054 doi:10.1093/procel/pwaf090 1674-8018 EMD-66854 consensus EM volume
consensus map
 EMD-66288 focused EM volume
local map
 9vif FULLOVERLAP EMDB EMD-66854 consensus EM volume
consensus map
 EMDB EMD-66288 focused EM volume
local map
 EMDB EMD-65089 associated EM volume
Cryo-EM Structure of NPFFR1 in complex with peptide NPFF
 Npffr1-Gi protein complex with NPFF Npffr1-Gi protein complex with NPFF 0 1 2 3 4 5 6 Homo sapiens Neuropeptide FF receptor 1 Homo sapiens 0.035150637 1 Trichoplusia ni LEVO NLTFSSYYQHTSPVAAMFIVAYALIFLLCMVGNTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITG WPFDNATCKMSGLVQGMSVSASVWTLVAIAVERFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTLTVTREEHH FMVDARNRSYPLYSCWEAWPEKGMRRVYTTVLFSHIYLAPLALIVVMYARIARKLCQAPGPAPGGEEAADPRASRRRARV VHMLVMVALFFTLSWLPLWALLLLIDYGQLSAPQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNENFRR Q9GZQ6 Guanine nucleotide-binding protein G(i) subunit alpha-1 Homo sapiens 0.039966438 1 Trichoplusia ni LEVO SAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAII RAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQP NYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHE SMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTC STDTKNVQFVFDAVTDVIIKNNLKDCGLF P63096 3.6.5.- Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Homo sapiens 0.037198656000000004 1 Trichoplusia ni LEVO ELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIW DSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFA TGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTD DGMAVATGSWDSFLKIWN P62873 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Homo sapiens 0.005931854 1 Trichoplusia ni LEVO SIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF P59768 scFv16 Homo sapiens 0.026466486 1 Trichoplusia ni LEVO DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLF LQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCR SSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG AGTKLELK NPFF Homo sapiens 0.001154338 1 Trichoplusia ni LEVO FLFQPQRFA singleParticle particle 7.4 ETHANE FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.7000000000000001 2.2 FEI FALCON IV (4k x 4k) 60.0 1 PHASE FLIPPING ONLY 3.19 FSC 0.143 CUT-OFF cryoSPARC 270038 COMMON LINE MAXIMUM LIKELIHOOD emd_65089.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 245.76 245.76 245.76 90.0 90.0 90.0 Z Y X -0.1294189 88.334519999999998 0.020154934 1.2327067 0.96 0.96 0.96 3.48 AUTHOR