Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp115, type B)

2026-03-04 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update PDBj PDBj 2025-09-03 2025-12-24 2025-12-24 2026-03-04 Japan Agency for Medical Research and Development (AMED) JP24ama121009 Japan Japan Society for the Promotion of Science (JSPS) JP20H05690 Japan Japan Society for the Promotion of Science (JSPS) JP24H00062 Japan Japan Society for the Promotion of Science (JSPS) JP20H03201 Japan Japan Society for the Promotion of Science (JSPS) JP23K17392 Japan Japan Society for the Promotion of Science (JSPS) JP22K15033 Japan Japan Society for the Promotion of Science (JSPS) JP24H02328 Japan Japan Society for the Promotion of Science (JSPS) JP23H05475 Japan Japan Society for the Promotion of Science (JSPS) JP20H05906 Japan Japan Science and Technology JPMJER1901 Japan Japan Science and Technology JPMJCR24T3 Japan Other private Daiichi Sankyo Foundation for Life Science Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp115, type B) Kujirai T Ehara H Ito T Henmi M Sekine S Kurumizaka H chromatin, nucleosome, TRANSCRIPTION Kujirai T Ehara H Ito T Henmi M Oya E Kobayashi T Sekine SI Kurumizaka H Structural basis of transcription-coupled H3K36 trimethylation by Set2 in coordination with FACT. Sci Adv US 12 eaed1952 eaed1952 2026 41604494 doi:10.1126/sciadv.aed1952 2375-2548 9wms FULLOVERLAP EMDB EMD-66103 associated EM volume

Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp115, type B) Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp115, type B) 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Komagataella phaffii DNA-directed RNA polymerase subunit Komagataella phaffii GS115 0.19410742199999997 1 LEVO MSQFPYSSAPLRSVKEVQFGLLSPEEIRAISVVKIEYPEIMDESRQRPREGGLNDPKLGSIDRNFKCQTCGEGMAECPGH FGHMELAKPVFHIGFIPKIKKVCECICMNCGKLLLDETNPTMAQAIRIRDPKKRFNAVWQLCKTKMVCEADAPVDEYSEQ KVVSRGGCGNTQPVVRKDGMKLWGTWKKSGFSDRDAQPERKLLTPGEILNVFKHISPEDCFRLGFNEDYARPEWMIITVL PVPPPQVRPSIAMDETTQGQDDLTHKLSDILKANINVQKLEMDGSPQHIINEVEQLLQFHVATYMDNDIAGQPQALQKSG RPVKAIRARLKGKEGRLRGNLMGKRVDFSARTVISGDPNLELDQVGVPISIAKTLSYPETVTQYNIHRLTEYVRNGPNEH PGAKYVIRDNGDRIDLRYHKRAGDIVLQYGWKVERHLMDDDPVLFNRQPSLHKMSMMAHRVKVMPYSTFRLNLSVTSPYN ADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPVMGIVQDTLCGVRKMTLRDTFIEYEQVMNMLFWVPSWDGV VPQPAILKPKPLWTGKQLLSIAIPSGIHLQRTDGGNSLLSPKDNGMLIVDGKVMFGVVDKKTVGSGGGGLIHTVMREKGP KICAELFGNIQKVVNYWLLHNGFSIGIGDAIADASTMKEITHAISSAKEQVQEIIYKAQHNELELKPGMTLRESFEGEVS RTLNDARDSAGRSAEMNLKDLNNVKQMVSAGSKGSFINIAQMSACVGQQMVEGKRIAFGFADRSLPHFTKDDFSPESKGF VENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDGTTRNSLGDIIQFLYGEDGLDGTQVE RQTIDTIPGSDKAFHKRYYVDLMDEKNSIKPDVIEYAADILGDVELQKELNSEYEQLVSDRKFLREIVFVNGDHNWPLPV NLRRIIQNAQQIFHLDRAKASDLTIPEIIHGVRDLCKKLFVLRGENELIKEAQQNATSLFQCLVRARLATRRILEEFRLN RDAFEWVLGTIEAQFQRSLVHPGEMVGVIAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPALTV YLDREIALDIEKAKVIQSSIEYTTLKNVTSATEIYYDPDPTSTVIEEDFDTVEAYFSIPDEKVEETIDKQSPWLLRLELD RARMLDKQLTMNQVADKISEVFSDDLFVMWSEDNADKLIIRCRVIRDPKAMDEELEAEEDQMLKRIEAHMLDLIALRGIP GISKVYMVKHKVSVPDESGEYKNEELWALETDGINLAEVMAVPGVDSSRTYSNSFVEILSVLGIEATRSSLYKEILNVIA FDGSYVNYRHMALLVDVMTSRGYLMAITRHGINRADTGALMRCSFEETVEILFEAGAAAELDDCRGVSENVMLGQLAPMG TGAFDVMIDEKLLTSLPADYAPTMPLFKGKATQGSATPYDNNAQYDDEFNHDDVADVMFSPMAETGSGDDRSGGLTEYAG IQSPYQPTSPGLSATSPGFAPTSPGFAPTSPRYSPTSPGYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP SYSPTSPSYSPTSPSYSPTSPSYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYS PTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPASPQYSPSRHSPNGESKEGE C4R4Y0 2.7.7.6 DNA-directed RNA polymerase subunit beta Komagataella phaffii GS115 0.13974609400000002 1 LEVO MSYDPYSIDDTITTEDCWTVISAFFEEKGLVSQQLDSFDEFMETSIQDLVWEEPRLILDQPAQHTNEKDNINKRYEIRFG KIYLSRPTMTEADGTTHAMFPQEARLRNLTYSSPVYLDMEKSMFTSIDDEGNPNATLDWQQVHEPIKDGVEEGNKVHIGK VPIMLRSKFCSLRTLDEVDLYKMKECPYDMGGYFVINGSEKVLIAQERSAANIVQVFKKAAPSPISHVAEIRSALEKGSR LISTMQIKLYGREDKGTGRTIKATLPYVKQDIPIVIVFRALGVVPDGEILQHICYDENDWQMLEMLKPCIEEGFVIQDKE VALDFIGRRGSAALGIRREKRIQYAKDILQKELLPHITQEEGFETRKTFFLGYMVNRLLLCALERKDQDDRDHFGKKRLD LAGPLLANLFRILFRKLTREIYRYMQRCIETDRDFNLNLAVKSTTITSGLKYSLATGNWGEQKKAMSSRAGVSQVLNRYT YSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLLSGISIGSPSEPIINFLEEWGMEPLE DYDPAQHTKSTRIFVNGVWTGIHRDPSMLVSTMRDLRRSGAISPEVSIIRDIREREFKIFTDVGRVYRPLFIVEDDESKD NKGELRITKEHIRKIQQGYDDDAMNDDSEEQEQDVYGWSSLVTSGVIEYVDGEEEETIMIAMTPEDLQTRSLEQKEIDLN DTAKRIKPEMSTSSHHTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYYPQ KPLAKTQAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKRFGISIVEEFEKPTRA TTLRLKHGTYEKLDEDGLIAPGVRVSGDDIIIGKTTPIPPDTEELGQRTKYHTKRDASTPLRSTENGIVDQVLLTTNQEG LKFVKVRMRTTKVPQIGDKFASRHGQKGTIGVTYRHEDMPFSAEGIVPDLIINPHAIPSRMTVAHLIECLLSKVGSIRGY EGDATPFTDLTVDAVSNLLRDNGYQSRGFEVMYNGHTGKKLMAQVFFGPTYYQRLRHMVDDKIHARARGPVQVLTRQPVE GRSRDGGLRFGEMERDCMIAHGAAGFLKERLMEASDAFRVHVCGICGLMSVIANLKKNQFECRSCKNKTNIYQLHIPYAA KLLFQELMAMNIAPRLYTERSGVSMRS C4QZQ7 2.7.7.6 RNA polymerase II third largest subunit B44, part of central core Komagataella phaffii GS115 0.034216292999999995 1 LEVO MSKEPKVNIINAQDDEVELMLSDVNLSLANSLRRTMLAEVPTLAIDLVEIKMNTSVLADEFISHRLGLIPLVSEDVEEMK YSRDCTCEDYCDECSVVLELSARHEGEEGTTDVYSSSLIKVSGPGNLNVGEPVRRDDYDQGILLCKLRNHQELNIRCIAK KGIAKEHAKWSPCSAIAFEYDPHNKLKHTDFWFEVDAKKEWPDSKYATWEEPPKPGEVFDYKAKPNRFYMTVETTGSLKA NQVFSRGIKTLQEKLANVLFELENSRPANTTAYGGATAYGGQTVYGRETSYGGNTNYGDYNAPY C4R7L2 RNA polymerase II subunit B32 Komagataella phaffii GS115 0.02062298 1 LEVO MNVSTSTVGARRRRAKQQVDDEENATLLRLGPEFALKQYDHDGNEHDLIALSLSESRLLIREALKARSRARNGGVDIESS NGEIDDDELAKVTSGAVANGVVKKTLDYLNTFARFKDEETCTAVDQLLHNSSDCSVLHPFEIAQLSSLGCEDVDEAITLI PSLAAKKEVNLQRILDELNRLEDPYK C4R2U9 DNA-directed RNA polymerases I, II, and III subunit RPABC1 Komagataella phaffii GS115 0.024962679999999998 1 LEVO MEDNNRIISRLWRSFRTVKEMAADRGYFISQEEMDQSLEEFRSKICDSMGNPQRKLMSFLANPTPEALEKYSDLGTLWVE FCDEPSVGIKTMRNFCLRIQEKNFSTGIFIYQNNITPSANKMIPTVSPAIIETFQESDLVVNITHHELVPKHIRLSDGEK SQLLQRYKLKESQLPRIQREDPVARYLGLKRGQVVKIIRRSETSGRYASYRICL C4R3P8 RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III Komagataella phaffii GS115 0.017803588 1 LEVO MSEDEAFNEQTENFENFEDEHFSDDNFEDRSTQPEDYAVGVTADGRQIINGDGIQEVNGTIKAHRKRSNKELAILKEERT TTPYLTKYERARILGTRALQISMNAPVLVDIEGETDPLQIAMKELSQRKIPLVIRRYLPDGSYEDWGCDELIVDN C4R1V1 RNA polymerase II subunit Komagataella phaffii GS115 0.018802625 1 LEVO MFFLKDLSLILTLHPSYFGPQMNQYLREKLLTDVEGTCTGQFGYIVTVLDGMNIDVGKGRIIPGSGSAEFEVKYRAVVWK PFKGEVVDAIVSNVSPIGFFADVGPLNVFVSTRLIPDNLVYNPSNSPPAYMSNDELITKGSKVRLKVVGTRTDVNEIYAI GSIKEDFLGAI C4R9A1 DNA-directed RNA polymerases I, II, and III subunit RPABC3 Komagataella phaffii GS115 0.016249219999999998 1 LEVO MSSALFDDIFTVQTVDNGRYNKVSRIIGISTTNSAIKLTLDINNEMFPVSQDDSLTVTLANSLSLDGEDESANFSKSWRP PKPTDKSLADDYDYVMFGTVYKFEEGDEDKIKVYVSFGGLLMCLEGGYKSLASLKQDNLYILIRR C4R273 DNA-directed RNA polymerase subunit Komagataella phaffii GS115 0.013612319999999999 1 LEVO MASFRFCLECNNMLYPKEDKENQRLLYSCRNCDYTELAEDPKVYRHELITNIGETAGIVDDIGQDPTLPRSDKECPECHS RDCVFFQSQQRRKDTNMTLFYVCLNCKKTFRDESE F2QPE6 RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Komagataella phaffii GS115 0.008554064 1 LEVO MIIPVRCFSCGKVVGDKWDAYLRLLEEGKQEGDALDELKLKRYCCRRMVLTHVDLIEKFLRYNPLEKKDFDS C4R009 RNA polymerase II subunit B12.5 Komagataella phaffii GS115 0.013832896 1 LEVO MNAPDRFELFILPDDVPKLKITPDSRVPNCIIIKFEREDHTLANLLREELALYPDVTFVAYKVEHPLFANFVMRLQTEEG TRPKQALERACASIINKLKTLDHKFNEEWNIKNFSLND C4R3Z5 RNA polymerase subunit ABC10-alpha Komagataella phaffii GS115 0.007862048 1 LEVO MSREGFVAPSGTDLAAAASGVAPNKHYGVKYTCGACAHNFSLNKSDPVRCKECGHRVIYKARTKRMIQFDAR F2QMI1 Transcription elongation factor 1 homolog Komagataella phaffii GS115 0.012606896 1 Escherichia coli LEVO GPGMGKRKSSARKPAPKIKQKLETQFTCLFCNHDNSVVCTLDKKNSIGLLECKKCNLSFQAPINSLSQPIDIYSDWIDAC EAVAEENADVNGDNFIENDGADREQDDDYDDEF C4QZ45 DNA (198-MER) synthetic construct 0.061264703 1 (DG)(DC)(DT)(DT)(DA)(DC)(DG)(DT)(DC)(DA)(DG)(DT)(DC)(DT)(DG)(DG)(DC)(DC)(DA)(DT) (DC)(DT)(DT)(DT)(DG)(DT)(DG)(DT)(DT)(DT)(DG)(DG)(DT)(DG)(DT)(DG)(DT)(DT)(DT)(DG) (DG)(DG)(DT)(DG)(DG)(DT)(DG)(DG)(DC)(DC)(DG)(DT)(DT)(DT)(DT)(DC)(DG)(DT)(DT)(DG) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DG)(DT)(DC)(DT)(DC)(DG)(DT)(DG)(DC)(DC)(DT) (DG)(DG)(DT)(DG)(DT)(DC)(DT)(DT)(DG)(DG)(DG)(DT)(DG)(DT)(DT)(DT)(DT)(DC)(DC)(DC) (DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DG)(DC)(DG)(DG)(DG) (DG)(DG)(DT)(DC)(DT)(DG)(DC)(DG)(DC)(DG)(DT)(DT)(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT) (DT)(DT)(DT)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DT)(DG)(DT)(DG)(DC)(DT)(DG)(DT)(DC) (DT)(DT)(DC)(DG)(DT)(DC)(DC)(DA)(DA)(DA)(DA)(DG)(DA)(DG)(DC)(DG)(DG)(DC)(DC)(DT) (DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT)(DC)(DT)(DG)(DA)(DT) DNA RNA (5'-R(P*GP*CP*UP*UP*GP*UP*GP*CP*UP*GP*UP*CP*UP*UP*CP*GP*UP*CP*CP*A)-3') synthetic construct 0.006290695 1 GCUUGUGCUGUCUUCGUCCA DNA (198-MER) synthetic construct 0.060987176 1 (DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DT)(DT)(DT)(DT)(DG)(DG)(DA)(DC)(DG)(DA)(DA)(DG)(DA) (DC)(DA)(DG)(DC)(DA)(DC)(DA)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DA)(DA)(DA)(DA)(DA) (DC)(DG)(DC)(DA)(DC)(DG)(DA)(DA)(DC)(DG)(DC)(DG)(DC)(DA)(DG)(DA)(DC)(DC)(DC)(DC) (DC)(DG)(DC)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)(DG) (DG)(DA)(DA)(DA)(DA)(DC)(DA)(DC)(DC)(DC)(DA)(DA)(DG)(DA)(DC)(DA)(DC)(DC)(DA)(DG) (DG)(DC)(DA)(DC)(DG)(DA)(DG)(DA)(DC)(DA)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DA) (DA)(DC)(DG)(DA)(DA)(DA)(DA)(DC)(DG)(DG)(DC)(DC)(DA)(DC)(DC)(DA)(DC)(DC)(DC)(DA) (DA)(DA)(DC)(DA)(DC)(DA)(DC)(DC)(DA)(DA)(DA)(DC)(DA)(DC)(DA)(DA)(DG)(DA)(DG)(DC) (DT)(DA)(DA)(DT)(DT)(DG)(DA)(DC)(DT)(DG)(DA)(DC)(DG)(DT)(DA)(DA)(DG)(DC) DNA Transcription elongation factor SPT4 Komagataella phaffii GS115 0.012039613999999999 1 Escherichia coli LEVO MRERACMLCGIVLPGRVFMQNGCPNCDSVLNLRDSDQATVNECTSSSFEGLVAVGDNEHSWVAKWLRVDRFQPGLYAVRV DGRLPSDIVAALEQYGVYYRPRDGSVID C4R0E6 Transcription elongation factor SPT5 Komagataella phaffii GS115 0.10145942200000001 1 Escherichia coli LEVO GPGMSETHKNQLDKVSTVSPDGPSEAVKEHSLQSKDLSKNDGQFIVPLDRNVIEQEEHKQVKSSAQAHNTTGDAADNEIE DGVPSEDVEFDKFKEDDYDEDDEVEEEGDIRSRKRRRHNQFLDVEAEVDDEEDDDDDDDDVELKHDFIQDDHIQHETQNE GFIAGHVDDDRLHRKLDQSREKIADQDAQELADEFKQRYGRSASSKYMGSASTTAPQRLLIPTVDDPGIWGVKVRLGKEK DVVRQILKKKLAREGTKNPLEIYSAFQRDSFKGHVYIEARKAEAINDALKGNVNVFSNNSKFLVGIVEYKDLLRPVKSSD VKLTRGSYVRVKNGKFKGDLAQVDEVLENGLEARLKLVPRLDYGKDLSHLSTSSSVDSTKNRRKFYTSKFRPAQRLFSEA EARVHEPTIRRDRDGFVTYGGEEYYEGFLYKTFRLQNLIVNSINPTLNELSLFQSNEESTTIDLSTIADSLKETAKNLVS FQPGDNVEIINGELNHLTGTVSSVNQSTIVSVRLHSDDDTINSETVEIPTSDLRKIFNVGDHVRVIHGKHTDDTGLIVEV NGDKVEFISNQTKRTVIVFSNYLIKSTDSTVSINESGRFELHDLVQVNSDLVGIVIRAQKDSFDVLCSDGKLLSLPPVSI YSKLNLNPNQQIAIDSNGVEVKVGDTVREFTGERRQGTILHVYRNFLFLRSREIVENQGVFVTSSNRVKTITSKSNGTGG QISGPDLSRMNPSRVIPPPSIPVANQRMTGRDPTLNKTVKIRQGGYKGKIGIVKEANGDRFRVELHNPNKTIPIPCSFLL IESTHGWVPYEDFVASDRRGGNIPRHEISGHVQQPQHGRAPAWGSGGKTPAWGSGGKTPAWGSGGSGGKTPAWGSGGKTP TWGSGAKTPAWGSGSKTPAWSGLDEEDRRDF C4R370 Transcription elongation factor Spt6 Komagataella phaffii GS115 0.17324162499999998 1 Spodoptera frugiperda LEVO GPGMSAPSPSVSEEDINETRNSIDADASTVQDLLDQDAQEGSGSDNEGSNIVDSSEEDDDEDDEEEMQKVREGFIVDDDD ENDEDGIPSSTKRKHRKKHKKRSAEVVEEVDEDDLQLLMENSGAVPGQQQNVKFKRLKRAEQDEKAQDSDSRGLNDMFSD EEGPGGVVEEGSEEEGLEDNLTTKTQRSGNLPHDEFDDFIEEDEFSDEDDEARDERLARMRSAKAAPQLAGLQGEQYQEI IEIFGDGTDYQWTLDAEEEMEQPQQDQEYDEAGEEIKGTTTSLADVFEPSELKEKMLTDEDNVIRVTDLPERFQAYRKSI KNYKLSDVDYSNERDWIVEQLKLEKRDFLQHLTQAHSSVAHLEEKFEASVKKIVDFIAIESFEVPFIWNHRRDYALHTYN DDSNNTIIVKLLNEDDLWRIVQLDLDYHSIHDKKAALSSIYKQLDLDVVDPTYEEFFGSARTLSELQDIDDYLTFNYSSQ VKNLTAVAELSIEGNGSGEDEEQTTKSSFAEVKMKRKYSKYAIYDRIRQDAIYPVVQSIANISQMRENLAQSKRLHQVED PIESPMDMIADIMSTEKDKTTFISSEKAYQAVKQFFSEQLSYEPFIRKTIRTAFQSFGVINIELTERGKLQIEPESPYFD FKYAKNRPISALTATPDLYLRMIQAENDGLVNIKVELPMLSTVVDHFYNILKSDGTSEISEKWNALRNDAWKQSLDKLIP LVQLNVKESIRRDCERVLYFQVKNSFTKKIDQAPYQPPTYAKGTIPRVLTLSFGEGNRGDAVLGVFMDDSGDVKSQIKFD EDFQSRDFSDSLTRYIKSNNINPDIIGISGFNIHTKKLFDKVNELVNEERLTIEYDNEYGYDREEDGRSDKHLIRVIYVN DETARLYQHSSKSSAEYPNRPQLAKYCIGLAKYIQSPLLEYLALDESMYSLHIHKHQNLLPREKLIDAVQTSIVDIVNLV GVDINEAVRAPYHALALPYVCGLGPRKAAGLIQSIQRIGSNLVNRAHLITEQLTSKTVFLNMASFVYIVFDPDVERNPQG EMDLLDSTRIHPEDYSLARKMAADALDIEDIDDDDESAMRNAIYEMVFPRSPPKDEDDLTFKLDELILDDYATELERKHQ LKKRSTLQIIKEELQSRYREIRRDFHILNEAEIFQLLTRETVDSFRKGMVIPVYVRKVESSYMSVSTQSLIAGNIQRQDI LEPNDRRDPREVYSVGQTVRACILDVDYYNFKCQLSLLRQFTENQVAGLNVNRNPKFWDIESENRDRQEEIDKQREESRE SRVIKHPFFHNMKSKEAEDYLAARPVGDVVIRPSSKGSNHITISWKVAPQLYQHIDVLEENKDDANAIGRVLLVGKYRYH DLDELLVEYVNNVANKVELMVSHDKFMSDSLDYVKEWLERYSKANGNRSHYIFTFNRKAPGWFFLLFKLNPTSEIKIWNV KALPDGYLLANNVYPDTNSLCNGFKTLMSSRRQIKQRSNRAGGEYNNSHAGAYDNGYSNAPRY C4R7H2 Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II Komagataella phaffii GS115 0.046907785 1 Escherichia coli LEVO GPGMSDEESENKKLLDQVLPEKTDQETQPEADAALAEQEAPENEPNEPASDDDSDSDLSDISDVDEEKVAEGLRYLDEQT AASLSKHKAAQPSSTHKKKEPTRRRPKQQTARETQYEPDVVEDEAARRTRLFEEKLDAAIKRKPNKRKKNDDVDLEQMQD ELIQQLKLQMEESAIRDANNIEQGKPAIFKLKLLPKVKDILLRANLADSILDNNLLASVRLWLEPLPDASLPAYQIQKVL FDAIKSLPIKTSHLRESGLGKVMVFYQKSKRVEPNLKRTAEKLISDWTRPIMGASDNYKDMRVRTQQFDPAQFAESLPGR VSVRPQEAKTLYEEAAERRKRAAIPQARTAAYTIAPQVNTELLMSSARRTLPSGVGSSLSGEDQYKRLNSRLNTMGTKRK SSAKKGGISIEGRGLPQ C4R7L8 Component of the Paf1p complex Komagataella phaffii GS115 0.124979008 1 Escherichia coli LEVO MKDHLIHNHHKHEHAHAEHDYKDDDDKEHLYFQGSSGSSGMSLLDQDYYLSALPEDKRAELVTLQLTIPLKSGDEVVTID FNEDVDVSQLCVLLESENARPDLWLSAAKVFVSKGNIAGSQEIIRKALQSNVILDSSASVTSLFHNFSFWLSLMEYVSTN SREDQFLEYASNSLQASNSLDSGLILNGIGNGVLYAKSRRYDEALKEFDNLLKKKSTNILAILGKAQILYKRQKYSQALE LYQKALTINPLIVPDPRLGIGLCFWHLNNKQLAEQAWHNSLKVHPQNNLNTKILICLAKFDYCFNESKDDDEFTALYRES LEFLHSCLKEDAKHPLLLMVLASYYFSKEDYEKVEKLCNLVLKENSRNAAFVSASHFWLARVAYHKEDYVQAQKQFKQAE DSQNSNTLAKLGYAQCLIARNEVGDATIYLEKFFKENQDSKSSEMMLLLGIIYSQSGKSYYKAIIFLEKYVAVCQEENYP ILPEAYLVLSRVYENKDLNVALDYLMKANDILGDKANVYVLNNLGIYHFFRNNVSQSSDFFAQSLEALNNVSPQNKEALS ITLHYNKARVEEVSNQSEAEKLYSKLMEKCPGYTSNKIRYIYLLALKSNGNNYADVQQLLDDFPSDLEVRSFYGWFLKRY GRKNGLKQDLESQHHKDTLINYDKHDCYALLSLGNIYATIAREMKVTDQKQNEIKRQQYLRAAQFYHKVLSIDPKNIYAA QGIAIIFSDKERTGLALEIFKKVRDTVQDLGTFINLGHCFMEAKQFGKAIESYTIALEKFSNGMDSKLLVLIGRAWYHRG FYEKSMDAYKKALEVSEQAYQLSKLPALRFNIVFIQFQIADFVKSLPNTQRDLTTLENALSGLNDAIKSLLKLAELEQPP YPSEDLKARATMGSNTLRNQLERAIQDQQDYEMSIQEKLRTARRKQQLDEEKRLEQEQRRLEEARKRQEAELIKRQELIK QAEEWNKMDIDAAKDNNDVLSEDGGEGEKKTTKKRKRKQKVSEDIAPDLDRINEENGNTEDYDPILDNEEEENYDEKDSE NKENGQNGQNEEVYPESEGDDEDDVTNTKRQKRSKGIVDEEDSE C4R6B2 RNAPII-associated chromatin remodeling Paf1 complex subunit Komagataella phaffii GS115 0.062301246 1 Escherichia coli LEVO MKDHLIHNHHKHEHAHAEHLYFQGSSGSSGMSDKEQDLDEDLLALAGAGSDSESNSAYSPEQEVSRKSKKIVNDSEDENN DSDGEQLLNPYPLEDKFKDEADRARLLGLSEMEREAILYERSQEVIKLKEKHLLSLRAKQSQLEKTAREGPKSSKTNKLS ELKKQREQKSKRQRSDEYSDEEDDYRYEEVGDNDDVEMSDNYIDEDDAVFVEPKKSSRSQELSKPATLSDINKIIFGRTA MSKYWYYPEFDDVVKGMYLRLNTGSGGNGFSPYKVVEVLGSQRIKGSAYGLNSKENNCDMYLKVAFPNQKEMVRPLFVFS DSSITHPEFDLFLRELDAEGLSVMDLRDVDYKYHQLKEMSSRSLSNDEVNSIVKMKQSLSSNTGFNTVLKKAQLQEELEE ARDAHDHERVARIEAELKSIGAESVVASKASSSMLKIDQRNKKLNNRFIRKAEMAAVEKRKLRGTSESDPFYRLATTARM FYKSANLGADDSNQPKTKAELEEEAKQEKKLEMQKLESMVKSNYRNGGLDRIISKIDFDFDLEL F2QQ42 Histone-lysine N-methyltransferase, H3 lysine-36 specific Komagataella phaffii GS115 0.083724547 1 Escherichia coli LEVO GPGMRERTVSDEEVDNYGHASHFKRKVQIYDYPADKTEEALTTFKTLSQNSYQNKTLGNAHNGRSTADGMSCDCEENWDT VSGINNACGEYSECINRLTSIECISGTCSCGDDCQNQRFQKKQYAPIAVFETEKKGYGVRAQADIRQDAFIYEYLGEVID ESTFRKRKENYDNQGLEHFYFMMLQKGEFIDATAKGGLGRFCNHSCRPNAYVDKWEVGNKLRMGIFAKREIYKGEEICFD YNVDRYGANPQKCYCGEDNCIGFLGGRTQTDSANILPVPIAEALGSTNAQEQRWVRLMKEQNKSIKASDYSSINEDYVNS LEMRPIADKDEASKVTSALLKTQDYIILRKLIERVSLTKDPEVLKEIVRLRGYQCVANILTMIVLDQEQQQVDVESFTLS VLDMLESWPNSSRNRISSSQIESVLESVKTKLKKIQPIGKKINSLLNSWSKLEISYKIPKSQSVGASNDVDNGHAESSTF QLRDEQKENNAASKISPFRIVDNMLLPEQWRWAVDESTGANYYYNKSQNITQWDLPPGCELLNIEVSEKNRDKKPPIDKS RLELKERQRKQEYERVKKEREREKLRLQREQEQEEQRRTETLKKIIANAEAKQQLLSKRLADEENEGKKRHKKSKRPTRE QEWNKLFATVVPNMIKKHESQIGRDNLKHCAKDIVHQLSSKEIKKDTSKKPPKELSSEKKAKVKAFVGPYMEKYLDKWKA KHEKK C4QY01 2.1.1.359 Leo1 Komagataella phaffii GS115 0.052387714999999994 1 Escherichia coli LEVO MKDHLIHNHHKHEHAHAEHLYFQGSSGSSGMSQELEQNRHSGEESPDPVLDANTSDVDDDGLDLFGDDDDEQPEAKPGQL DDSSNDAEEDEQEKAGKPEAKVTSHTKSVYDNGETLDISLPIHPKSHIPQGKQDRWVVKLPDFLDINAEPFDPRPFEMNV KTHEDKNQELLDKLIAVNTVRWRYAKSETGGIFKETNSQIIQWEDGTYSLRVGSEIFDMFTTNTDDNYLVSEHNEEGILM TESTLSKSVKLVPASFQSTTHQKLAKALSAKQKKESYARSVVTKEDPEERQRRLESQENERYRLERRRKQAEEEEDYDSY AVSTSRSRPSRASQMYNAADEDDDDDDVAIGRTLASRRNNGYEDDGFIIDDEEEEEAVENDSADEESENEGDDDEEDDEE AAERLNRLKRAGASKYTNEGEQAQSEPVKSEEADNASQRDDAPRKKRRVILDDDEEEEE C4R3K1 RNAP II-associated protein Komagataella phaffii GS115 0.04604598 1 Escherichia coli LEVO GSAMTSSKSRQDYIAKVRYQNDLPAPPCPPKLLKYEIEKEAPQKEFLKDSRLLSALFSKDNFRYLMNETSDGLDVNYLRI PGIIENEKSLGKLFSSYKNLAIENLHPDDRLLLVDPNKSATLNEESPVFFLRRPQYVSDGEKINLQNFTNKRKHQDMEDT NPRSQLHSVERTFDEVIDPRNKNRLQSLIHPRKKIKAVKAWHFFPDTSTFDQVFHSLKFVGSASLSKDRPLNEQLGQVSE TDSTSVNASILTSLFKPIEINPHNKWISLYAVTDKLSAESFRKSFNSIKDDNIVNRHVIYDHIKDFDQMFRGHKKLFEDF AISFDDISDRAFFVPIVGRLELKKKRIVPGLVDMVNRTNYAHIRMDLRNPSTQETAIRDSRREQYDPVNYSSIQEE C4R997 Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p Komagataella phaffii GS115 0.044760652 1 Escherichia coli LEVO MKDHLIHNHHKHEHAHAEHLYFQGSSGSSGMSEPLIALRQAIKDKKPVGVNEGETIGNAKVLNIGEKQYSLDAPTSFVIN GKEFNLKVIYQCWVFRDSSSADYITECEKENIDGISFVERSELISWLKGEITSSAFIKGEKVGITEENGKNEGETKSNNK RKLSDDPLLKEIASNERVLIDHNKVLRGLKPKDFSSVAKDCELRILKEKPANAKSSDSNGRSSTSVSSSSKDARNKEPII VLSPAASSLVRMSNVKEFLQEGKFLDPSKEPASSSNLLAIQRKSSRFKTPIKLLVVDNVEKLFTKSEYWDRVVAIVTTGK DWQFKNYKYKDPQILFQKFNGFYFKYKGDAVPASVKSWNVKVLDIDRVERFSDRQVVEQFWDTVENTLVAKRYKS C4R1E6 Histone H3.3 Homo sapiens 0.015645277 2 Escherichia coli LEVO GSHMARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVMKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQ DFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA P84243 Histone H4 Homo sapiens 0.011676702999999998 2 Escherichia coli LEVO GSHMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHA KRKTVTAMDVVYALKRQGRTLYGFGG P62805 Histone H2A type 1-B/E Homo sapiens 0.014447825 2 Escherichia coli LEVO GSHMSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKT RIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK P04908 Histone H2B type 1-J Homo sapiens 0.014191478999999998 2 Escherichia coli LEVO GSHMPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGE ASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTCYTSAK P06899 ZINC ION 6.5409e-05 13 ZN MAGNESIUM ION 2.4305e-05 1 MG S-ADENOSYL-L-HOMOCYSTEINE 0.000384411 1 SAH singleParticle particle 7.5 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.2 2.0 GATAN K3 BIOQUANTUM (6k x 4k) 58.0 1 NONE C1 3.4 FSC 0.143 CUT-OFF 59019 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_66103.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 270 270 270 0 0 0 270 270 270 398.25 398.25 398.25 90.0 90.0 90.0 X Y Z -0.062867545 0.16699721 0.00036139245 0.0048723207 1.475 1.475 1.475 0.015 AUTHOR ::::EMDATABANK.org::::EMD-66103:::: emd_66103_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 220 220 220 0 0 0 220 220 220 324.5 324.5 324.5 90.0 90.0 90.0 X Y Z -0.033727728 0.09418852 0.00070476305 0.0031656085 1.475 1.475 1.475 AUTHOR ::::EMDATABANK.org::::EMD-66103:::: upstream reconstruction emd_66103_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 220 220 220 0 0 0 220 220 220 324.5 324.5 324.5 90.0 90.0 90.0 X Y Z -0.03868402 0.106713735 0.0006066449 0.003144798 1.475 1.475 1.475 AUTHOR ::::EMDATABANK.org::::EMD-66103:::: nucleosome reconstruction emd_66103_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 270 270 270 0 0 0 270 270 270 398.25 398.25 398.25 90.0 90.0 90.0 X Y Z -0.08429712 0.18764575 0.00036020103 0.0077841096 1.475 1.475 1.475 AUTHOR ::::EMDATABANK.org::::EMD-66103:::: emd_66103_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 270 270 270 0 0 0 270 270 270 398.25 398.25 398.25 90.0 90.0 90.0 X Y Z -0.088202015 0.18063398 0.00036273926 0.0077833547 1.475 1.475 1.475 AUTHOR ::::EMDATABANK.org::::EMD-66103::::