Cryo-EM structure of DAMGO-muOR-Gz-scFv16 complex

2025-12-24 REL PDBc INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation_author.identifier_ORCID _em_admin.last_update PDBj PDBc 2025-09-15 2025-11-26 2025-11-26 2025-12-24 Not funded Cryo-EM structure of DAMGO-muOR-Gz-scFv16 complex Zhang H Wang X Xi K Shen Q Xue J Zhu Y Yang G Zhang Y G-protein-coupled receptors, mu-opioid receptor, single particle, Cryo-EM, MEMBRANE PROTEIN Zhang H Wang X Xi K Shen Q Xue J Zhu Y Zang SK Yu T Shen DD Guo J Chen LN Ji SY Qin J Dong Y Zhao M Yang M Wu H Yang G Zhang Y The molecular basis of mu-opioid receptor signaling plasticity. Cell Res. CN 35 1021 1036 2025 41199005 doi:10.1038/s41422-025-01191-8 1001-0602 9wst FULLOVERLAP EMDB EMD-66205 associated EM volume

Complex of DAMGO bound MOR-Gz protein Complex of DAMGO bound MOR-Gz protein 0 1 2 3 4 Homo sapiens Engineered guanine nucleotide-binding protein G(z) subunit alpha Homo sapiens 0.040699516 1 Spodoptera frugiperda LEVO MGCTLSAEDKAAVERSKMIDRNLREDGEKQRREIKLLLLGTSNSGKNTIVKQMKIIHSGGFNLEACKEYKPLIIYNAIDS LTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADPGAQACFSRSSEYHLEDNAAYYLNDL ERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGAQRSERKKWIHCFEGVTAIIFCVELSGYDLKLYEDNQ TSRMAASLKLFDSICNNKWFIDTSLILFLNKKDLLAEKIRRIPLTICFPEYKGQNTYEEAAVYIQRQFEDLNRNKETKEI YSHFTCSTDTSNIQFVFDAVTDVIIQNNLKYIGLC Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Homo sapiens 0.040039648 1 Spodoptera frugiperda LEVO GPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQ DGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDN QIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRV SCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWRLFKKIS P62873 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Homo sapiens 0.007861143 1 Spodoptera frugiperda LEVO MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL P59768 Mu-type opioid receptor,LgBiT Oplophorus gracilirostris 0.064018785 1 Spodoptera frugiperda LEVO DYKDDDDKGPGNISDCSDPLAPASCSPAPSPGSWLNLSHVDGNQSDPCGPNRTGLGGSHSLCPQTGSPSMVTAITIMALY SIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGNILCKIVISIDYYNMFTSI FTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKIC VFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQT VSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSARIRQNTREHPSTANTVDRTNHQLENLEAETA PLPGGSSGVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMA QIEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLF RVTINSGGSLEVLFQ P35372 DAMGO synthetic construct 0.0005135869999999999 1 LEVO Y(DAL)G(MEA)(ETA) water 1.8015e-05 1 HOH singleParticle particle 7.4 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.6 2.0 FEI FALCON IV (4k x 4k) 52.0 1 NONE 2.8 FSC 0.143 CUT-OFF RELION 136606 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_66205.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 208.32 208.32 208.32 90.0 90.0 90.0 X Y Z -0.023929298 0.04379716 0.0000012071813 0.0011999487 0.93 0.93 0.93 0.005 AUTHOR ::::EMDATABANK.org::::EMD-66205:::: emd_66205_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 208.32 208.32 208.32 90.0 90.0 90.0 X Y Z -0.016338302 0.031154776 0.0000017197682 0.0010879949 0.93 0.93 0.93 AUTHOR ::::EMDATABANK.org::::EMD-66205:::: emd_66205_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 0 0 0 224 224 224 208.32 208.32 208.32 90.0 90.0 90.0 X Y Z -0.016541798 0.032212436 0.00000069459435 0.001074238 0.93 0.93 0.93 AUTHOR ::::EMDATABANK.org::::EMD-66205::::