2026-03-04 REL PDBc INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBc 2025-09-24 2025-12-17 2025-12-17 2026-03-04 Other government 24ZR1403800 China Japan Science and Technology JPMJPR20E1 Japan Tomogram of doublet microtubules with bound dynein-2 molecules He HK Chen X Lv QH Ichikawa M cilia, intraflagellar transport, doublet microtubule, dynein-2, MOTOR PROTEIN He HK Kubo S Chen X Lv QH Kage A Ichikawa M Cryo-ET and MD simulations reveal that dynein-2 is tuned for binding to the A-tubule of the ciliary doublet. Embo J. UK 44 7677 7701 2025 41299077 doi:10.1038/s44318-025-00648-1 1460-2075 0897 EMJODG EMDB EMD-66326 associated EM volume Complex of doublet microtubules and GST-Dyn2 molecules Complex of doublet microtubules and GST-Dyn2 molecules 0 Tetrahymena thermophila SB255 Cilia SNAP-GST-Dyn2 1
Dynein-2 heavy chain's N-terminal tail domain was truncated and replaced with GST-tag for dimerization. There is also SNAP-tag attached at the N-terminal side.
Homo sapiens Cilia Doublet microtubules 1 Tetrahymena thermophila SB255 Cilia tomography filament 6.9 Quantifoil R2/2 COPPER GLOW DISCHARGE ETHANE FEI VITROBOT MARK IV Sigma-Aldrich 10 NO SECTIONING TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.5 0.5 29000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE FEI FALCON II (4k x 4k) 26 3.065 1 SIMULTANEOUS ITERATIVE (SIRT) IMOD 26 NONE emd_66326.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 1366 200 1366 0 305 0 1366 200 1366 11647.883 1705.4001 11647.883 90.0 90.0 90.0 X Y Z -18071.360000000000582 14108.575999999999112 2182.735799999999927 651.99676999999997 8.527 8.527 8.527 AUTHOR