Human 80S ribosome bound to IDB-001 stalled on MYC nascent chain

2026-03-11 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.pdbx_database_id_PubMed _citation.title _citation_author.identifier_ORCID _citation_author.name _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation.pdbx_database_id_PubMed _citation.title _citation_author.identifier_ORCID _citation_author.name _em_admin.last_update RCSB RCSB 2025-04-07 2026-03-04 2026-03-04 2026-03-11 Not funded Human 80S ribosome bound to IDB-001 stalled on MYC nascent chain Sauer PV Schuller AP Hamann LG translation inhibitor, interdictor, ribosome, cryogenic-electron microscopy (cryo-EM), ribotoxic stress response, cancer, MYC, TRANSLATION Diamond PD Sauer PV Holm M Swanson-Swett CJ Ferguson L Bratset NM Wienker GW Sim JS Adams HK Kenner L Meyers M Gygi D Konst ZA Bahmanyar SS Hamann LG Schuller AP Context-dependent translation inhibition as a cancer therapeutic modality. Nat Commun UK 17 2026 41735331 doi:10.1038/s41467-026-69891-2 2041-1723 9o3w FULLOVERLAP EMDB EMD-70084 associated EM volume

Human 80S ribosome bound to IDB-001 stalled on MYC nascent chain Human 80S ribosome bound to IDB-001 stalled on MYC nascent chain 0 46 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 47 48 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 Homo sapiens 4.3 28S rRNA Homo sapiens 1.6408844999999999 1 CGCGACCUCAGAUCAGACGUGGCGACCCGCUGAAUUUAAGCAUAUUAGUCAGCGGAGGAGAAGAAACUAACCAGGAUUCC CUCAGUAACGGCGAGUGAACAGGGAAGAGCCCAGCGCCGAAUCCCCGCCCCGCGGCGGGGCGCGGGACAUGUGGCGUACG GAAGACCCGCUCCCCGGCGCCGCUCGUGGGGGGCCCAAGUCCUUCUGAUCGAGGCCCAGCCCGUGGACGGUGUGAGGCCG GUAGCGGCCCCCGGCGCGCCGGGCCCGGGUCUUCCCGGAGUCGGGUUGCUUGGGAAUGCAGCCCAAAGCGGGUGGUAAAC UCCAUCUAAGGCUAAAUACCGGCACGAGACCGAUAGUCAACAAGUACCGUAAGGGAAAGUUGAAAAGAACUUUGAAG (A2M)G(A2M)GAGUUCAAGAGGGCGUGAAACCGUUAAGAGGUAAACGGGUGGGGUCCGCGCAGUCCGCCCGGAGGAUUC AACCCGGCGGCGGGUCCGGCCGUGUCGGCGGCCCGGCGGAUCUUUCCCGCCCCCCGUUCCUCCCGACCCCUCCACCCGCC CUCCCUUCCCCCGCCGCCCCUCCUCCUCCUCCCCGGAGGGGGCGGGCUCCGGCGGGUGCGGGGGUGGGCGGGCGGGGCCG GGGGUGGGGUCGGCGGGGGACCGUCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCAUUUCCACCGCGGCGGUGCGCCGC GACCGGCUCCGGGACGGCUGGGAAGGCCCGGCGGGGAAGGUGGCUCGGGGGGCCCCGUCCGUCCGUCCGUCCGUCCUCCU CCUCCCCCGUCUCCGCCCCCCGGCCCCGCGUCCUCCCUCGGGAGGGCGCGCGGGUCGGGGCGGCGGCGGCGGCGGCGGUG GCGGCGGCGGCGGCGGCGGCGGGACCGAAACCCCCCCCGAGUGUUACAGCCCCCCCGGCAGCAGCACUCGCCGAAUCCCG GGGCCGAGGGAGCGAGACCCGUCGCCGCGCUCUCCCCCCUCCCGGCGCCCACCCCCGCGGGGAAUCCCCCGCGAGGGGGG UCUCCCCCGCGGGGGCGCGCCGGCGUCUCCUCGUGGGGGGGCCGGGCCACCCCUCCCACGGCGCGACCGCUCUCCCACCC CUCCUCCCCGCGCCCCCGCCCCGGCGACGGGGGGGGUGCCGCGCGCGGGUCGGGGGGCGGGGCGGACUGUCCCCAGUGCG CCCCGGGCGGGUCGCGCCGUCGGGCCCGGGGGAGGUUCUCUCGGGGCCACGCGCGCGUCCCCCGAAGAGGGGGACGGCGG AGCGAGCGCACGGGGUCGGCGGCGACGUCGGCUACCCACCCGACCC(OMG)UCUUG(1MA)AAC(A2M)CGGACCAAGGA GU(OMC)UAACACGUGCGCGAGUCGGGGGCUCGCACGAAAGCCGCCGUGGCGCAAUGAAGGUGAAGGCCGGCGCGCUCGC CGGCCGAGGUGGGAUCCCGAGGCCUCUCCAGUCCGCCGAGGGCGCACCACCGGCCCGUCUCGCCCGCCGCGCCGGGGAGG UGGAGCACGAGCGCACGUGUUAGGACCC(OMG)A(A2M)AGAUGGUGA(A2M)C(PSU)AUGCCUGGGCAGGGCGAAGCC AGAGGAAACUCUGGUGGAGGUCCG(PSU)AGCGGUCCUGACGUGCAAAUCGGUCGUCCGACCUGGGUAUAG(OMG)GGCG AAAGACUAAUCGAACCAUCUAGUAGCUGGUUCCCUCCGAAGUUUCCC(PSU)CAGGA(PSU)AGCUGGCGCUCUCGCAGA CCCGACGCACCCCCGCCACGCAGUUUUAUCCGGUAAAGCGAA(PSU)GAUUAGAGGUCUUGGGGCCGAAACGAUCUCAAC C(PSU)A(PSU)(PSU)CUCAAACUU(PSU)AAAUGGGUAAGAAGCCCGGCUCGCUGGCGUGGAGCCGGGCGUGGAAUGC GAGUGCCUAGUGGGCCAC(PSU)U(PSU)UGGUAAGC(A2M)GAACUGGCG(OMC)UGCGGGAUGAACCGAACGCCGGGU UAAGGCGCCCGAUGCCGACGCUCAUCAGACCCCAGAAAAGGUGUUGGUUGAUAUAGACAGCAGGACGGUGGCCAUGGAAG UCGGAAUCCGCUAAGGAGUGUGUAACAACUCACCUGCCGAAUCAACUAGCCCUGAAAAUGGAUGGCGCUGGAGCGUCGGG CCCAUACCCGGCCGUCGCCGGCAGUCGAGAGUGGACGGGAGCGGCGGGGGCGGCGCGCGCGCGCGCGCGUGUGGUGUGCG UCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGUGUGGGGUCCUUCCCCCGCCCCCCCCCCCACGCCUCCUCCCCUCCUCC CGCCCACGCCCCGCUCCCCGCCCCCGGAGCCCCGCGGACGCUACGCCGCGACGAGUAGGAGGGCCGCUGCGGUGAGCCUU GAAGCCUAGGGCGCGGGCCCGGGUGGAGCCGCCGCAGGUGCAGAU(OMC)UUGGUGGUAGU(A2M)(OMG)(OMC)AAAU AUUCAAACGAGAACUUUGAAGGCCGAAGUGG(A2M)GAAGGGUUCCAUG(OMU)GAACAG(OMC)A(OMG)UUGAACAUG GGUCAGUCGGUCCUGAGAGAUGGGCGAGCGCCGUUCCGAAGGGACGGGCGAUGGCCUCCGUUGCCCUCGGCCGA(PSU)C GAAAGGGAGUCGGGUUCAGAUCCCCGAAUCCGGAGUGGCGGAGAUGGGCGCCGCGAGGCGUCCAGUGCGGUAACGCGACC GAUCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGUUCUCUU(PSU)UCUUUGUGAAGGGCAGGGCGCCCUGGAAUGGGU UCGCCCCGAGAGAGGGGCCCGUGCCUUGGAAAGCGUCGCGGUUCCGGCGGCGUCCGGUGAGCUCUCGCUGGCCCUUGAAA AUCCGGGGGAGAGGGUGUAAAUCUCGCGCCGGGCCGUACCC(A2M)UAUCCGCAGCAGGUCU(OMC)CAAGGUGAAC (A2M)GCCUCUGG(OMC)AUGUUGGAACAA(OMU)G(PSU)AGG(PSU)AAGGGAAGUCGGCAAGC(OMC)GGAUCCGUA ACUUC(OMG)GGAUAAGGAUUGGCUCUAAGGGCUGGGUCGGUCGGGCUGGGGCGCGAAGCGGGGCUGGGCGCGCGCCGCG GCUGGACGAGGCGCCGCCGCCCCCCCCACGCCCGGGGCACCCCCCUCGCGGCCCUCCCCCGCCCCACCCCGCGCGCGCCG CUCGCUCCCUCCCCGCCCCGCGCCCUCUCUCUCUCUCUCUCCCCCGCUCCCCGUCCUCCCCCCUCCCCGGGGGAGCGCCG CGUGGGGGCGGCGGCGGGGGGAGAAGGGUCGGGGCGGCAGGGGCCGGCGGCGGCCCGCCGCGGGGCCCCGGCGGCGGGGG CACGGUCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACUCUGGACGCGAGCCGGGCCCUUCCC GUGGAUCGCCCCAGCUGCGGCGGGCGUCGCGGCCGCCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCCCCCCCCACCC CACGUCUCGUCGCGCGCGCGUCCGCUGGGGGCGGGGAGCGGUCGGGCGGCGGCGGUCGGCGGGCGGCGGGGCGGGGCGGU UCGUCCCCCCGCCCUACCCCCCCGGCCCCGUCCGCCCCCCGUUCCCCCCUCCUCCUCGGCGCGCGGCGGCGGCGGCGGCA GGCGGCGGAGGGGCCGCGGGCCGGUCCCCCCCGCCGGGUCCGCCCCCGGGGCCGCGGUUCCGCGCGGCGCCUCGCCUCGG CCGGCGCCUAGCAGCCGACUUAGAACUGGUGCGGACCAGG(OMG)GAAUCCGAC(PSU)G(PSU)UUAAUUAAAACAAAG CAUCGCGAAGGCCCGCGGCGGGUGUUGACGCGAUGUGAUU(PSU)CUGCC(OMC)AGUGCUCUGAAUG(PSU)CA(A2M) AGUG(A2M)(A2M)GAAA(PSU)UCAA(PSU)GAAGCGCGG(OMG)UAAACGGCGGGAG(PSU)A(A2M)C(PSU)A (PSU)GAC(PSU)C(PSU)CUUAAGGUAGC(5MC)AA(A2M)UGCCUC(OMG)UCAUCUAAUUAGUGA(OMC)GCGCAUG AA(UY1)GGAUGA(A2M)CGAG(A2M)UUCCCACUGU(OMC)CC(PSU)ACCUAC(PSU)A(PSU)CCAGCGAAACCAC (A2M)G(OMC)CAAGGGAACGGGCU(PSU)GG(OMC)GGAAUCAGCGG(OMG)GAAAGAAGACCCUGUUGAGC(PSU)UG AC(OMU)CUAGUCUGGCACGGUGAA(OMG)AGACAUGAGAGGUGUAGAAUAAGUGGGAGGCCCCCGGCGCCCCCCCGGUG UCCCCGCGAGGGGCCCGGGGCGGGGUCCGCCGGCCCUGCGGGCCGCCGGUGAAAUACCACUACUCUGAUCGUUUUUUCAC UGACCCGGUGAGGCGGGGGGGCGAGCCCCGAGGGGCUCUCGCUUCUGGCGCCAAGCGCCCGGCCGCGCGCCGGCCGGGCG CGACCCGCUCCGGGGACAGUGCCAGGUGGGGAGUUUGACUG(OMG)GGCGGUACACCUGUCAAACGGUA(6MZ)CGCAGG (OMU)(OMG)UCCUAAGGCGAGCUCAGGGAGGACAGAAACCUCCCGUGGAGCAGAAGGGCAAAAGCUCGCUUGA(PSU)C U(PSU)GA(PSU)UUUCAG(OMU)ACGAA(PSU)ACAGACCGUGAAAGCGGGGCCUCACGAUCCUUCUGACCUU(PSU)U GGGUUU(PSU)AAGCAGGA(OMG)GUGUCAGAAAAGUUACCACAG(OMG)GAUAACUGGC(PSU)UGUGGCGGCCAAGCG U(PSU)CA(PSU)AGCGACG(PSU)CGCUUUUUGA(PSU)CCUU(5MC)GAUGUCGG(OMC)(PSU)CUUCCUAUCAUUG (PSU)GAAGCAGAAUUCACCAAGCGU(PSU)(OMG)GAU(OMU)(OMG)(PSU)UCACCCACUAAUAGGGAACG(PSU)G (A2M)GCUGGG(UR3)(PSU)(PSU)AGA(OMC)CGUCGUGAGACAGGU(PSU)AGUUUUACCCUACUGA(PSU)G (A2M)UGUG(PSU)UG(PSU)UGCCAUGGUA(A2M)UCCUGCUCAGUACGAGAGGAACCGCAG(OMG)U(OMU)CA (OMG)ACAU(PSU)UGGUGUA(PSU)(OMG)UGCUUGGCUGAGGAGCCAAUGGGGCGAAGCUACCA(PSU)CUGUGGGAU UAUGAC(PSU)GAACGCCUCUAAGUCAGAAUCCCGCCCAGGCGGAACGAUACGGCAGCGCCGCGGAGCCUCGGUUGGCCU CGGAUAGCCGGUCCCCCGCCUGUCCCCGCCGGCGGGCCGCCCCCCCCCUCCACGCGCCCCGCGCGCGCGGGAGGGCGCGU GCCCCGCCGCGCGCCGGGACCGGGGUCCGGUGCGGAGUGCCCUUCGUCCUGGGAAACGGGGCGCGGCCGGAGAGGCGGCC GCCCCCUCGCCCGUCACGCACCGCACGUUCGUGGGGAACCUGGCGCUAAACCA(PSU)UCGUAGACGACCUGCUUCUGGG UCGGGG(PSU)UUCGUACG(PSU)AGCAGAGCAGCUCCCUCGCUGCGAUCUAUUGAAAGUCAGCCCUCGACACAAGGGUU UGU 5S rRNA Homo sapiens 0.038691913999999994 1 GUCUACGGCCAUACCACCCUGAACGCGCCCGAUCUCGUCUGAUCUCGGAAGCUAAGCAGGGUCGGGCCUGGUUAGUACUU GGAUGGGAGACCGCCUGGGAAUACCGGGUGCUGUAGGCUU X12811.1 5.8S rRNA Homo sapiens 0.050171703 1 CGACUCUUAGCGG(OMU)GGAUCACUCGGCUCGUGCGUCGAUGAAGAACGCAGCUAGC(PSU)GCGAGAAUUAAUG (PSU)GAAUU(OMG)CAGGACACAUUGAUCAUCGACACUUCGAACGCACUUGCGGCCCCGGGUUCCUCCCGGGGCUACGC CUGUCUGAGCGUCGCU U13369.1 60S ribosomal protein L8 Homo sapiens 0.028088863 1 LEVO MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAE GIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISS ANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAA RRTGRLRGTKTVQEKEN P62917 Large ribosomal subunit protein uL3 Homo sapiens 0.046224133 1 LEVO MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVE TPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDEDGKKQLEKDFSSMKKYCQVI RVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKL PRKT(HIC)RGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINP LGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDR IAKEEGA P39023 Large ribosomal subunit protein uL4 Homo sapiens 0.047804621 1 LEVO MACARPLISVYSEKGESSGKNVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVR GGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEG YKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKL APGGHVGRFCIWTESAFRKLDELYGTWRKAASLKSNYNLPMHKMINTDLSRILKSPEIQRALRAPRKKIHRRVLKKNPLK NLRIMLKLNPYAKTMRRNTILRQARNHKLRVDKAAAAAAALQAKSDEKAAVAGKKPVVGKKGKKAAVGVKKQKKPLVGKK AAATKKPAPEKKPAEKKPTTEEKKPAA P36578 Large ribosomal subunit protein uL18 Homo sapiens 0.03442678899999999 1 LEVO MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYA HELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVF GALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMY KKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES P46777 Large ribosomal subunit protein eL6 Homo sapiens 0.032810175999999996 1 LEVO MAGEKVEKPDTKEKKPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGIGRYSRSAMYSRKAMYKRKYSAAKSKV EKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSQHVRKLRASITPGTILIILTGRHRGK RVVFLKQLASGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISNVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKY EITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNGIYPHKLVF Q02878 60S ribosomal protein L7 Homo sapiens 0.029290973 1 LEVO MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGN FYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYG KINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQI NRLIRRMN P18124 Large ribosomal subunit protein eL8 Homo sapiens 0.030061784999999997 1 LEVO MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAI NQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHD VDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGN VLGPKSVARIAKLEKAKAKELATKLG P62424 Large ribosomal subunit protein uL6 Homo sapiens 0.021899471 1 LEVO MKTILSNQTVDIPENVDITLKGRTVIVKGPRGTLRRDFNHINVELSLLGKKKKRLRVDKWWGNRKELATVRTICSHVQNM IKGVTLGFRYKMRSVYAHFPINVVIQENGSLVEIRNFLGEKYIRRVRMRPGVACSVSQAQKDELILEGNDIELVSNSAAL IQQATTVKNKDIRKFLDGIYVSEKGTVQQADE P32969 Large ribosomal subunit protein uL16 Homo sapiens 0.024630061 1 LEVO MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSC GKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFP GRQKIHISKKWGFTKFNADEFEDMVAEKRLIPDGCGVKYIPSRGPLDKWRALHS P27635 Large ribosomal subunit protein uL5 Homo sapiens 0.020288465 1 LEVO MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAE EILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKE EAMRWFQQKYDGIILPGK P62913 Large ribosomal subunit protein eL13 Homo sapiens 0.024321682 1 LEVO MAPSRNGMVLKPHFHKDWQRRVATWFNQPARKIRRRKARQAKARRIAPRPASGPIRPIVRCPTVRYHTKVRAGRGFSLEE LRVAGIHKKVARTIGISVDPRRRNKSTESLQANVQRLKEYRSKLILFPRKPSAPKKGDSSAEELKLATQLTGPVMPVRNV YKKEKARVITEEEKNFKAFASLRMARANARLFGIRAKRAKEAAEQDVEKKK P26373 Large ribosomal subunit protein eL14 Homo sapiens 0.023485015999999997 1 LEVO MVFRRFVEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKA DINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEVKKLQKAALLKASPKKAPGTKGTAAAAAAAAAAK VPAKKITAASKKAPAQKVPAQKATGQKAAPAPKAQKGQKAPAQKAPAPKASGKKA P50914 Large ribosomal subunit protein eL15 Homo sapiens 0.024207285 1 LEVO MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGAT YGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHRE MRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR P61313 Large ribosomal subunit protein uL13 Homo sapiens 0.023633412 1 LEVO MAEVQVLVLDGRGHLLGRLAAIVAKQVLLGRKVVVVRCEGINISGNFYRNKLKYLAFLRKRMNTNPSRGPYHFRAPSRIF WRTVRGMLPHKTKRGQAALDRLKVFDGIPPPYDKKKRMVVPAALKVVRLKPTRKFAYLGRLAHEVGWKYQAVTATLEEKR KEKAKIHYRKKKQLMRLRKQAEKNVEKKIDKYTEVLKTHGLLV P40429 Large ribosomal subunit protein uL22 Homo sapiens 0.021443169999999998 1 LEVO MVRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDVTLQKQCVPFRRYNGGVGRCAQAKQWGWTQ GRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKEQIVPKP EEEVAQKKKISQKKLKKQKLMARE P18621 Large ribosomal subunit protein eL18 Homo sapiens 0.021687676 1 LEVO MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTA VVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPH SHTKPYVRSKGRKFERARGRRASRGYKN Q07020 Large ribosomal subunit protein eL19 Homo sapiens 0.023535280999999998 1 LEVO MSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVHSRARCRKNTLARRKGRHMGIGK RKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDRHMYHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAE ARRSKTKEARKRREERLQAKKEEIIKTLSKEEETKK P84098 Large ribosomal subunit protein eL20 Homo sapiens 0.020808513999999997 1 LEVO MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGI WLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLR RQHKPRFTTKRPNTFF Q02543 Large ribosomal subunit protein eL21 Homo sapiens 0.018609988 1 LEVO MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQV KGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA P46778 Large ribosomal subunit protein eL22 Homo sapiens 0.014813014999999999 1 LEVO MAPVKKLVVKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSK RYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQINQDEEEEEDED P35268 Large ribosomal subunit protein uL14 Homo sapiens 0.014892504999999999 1 LEVO MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAV VIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA P62829 Large ribosomal subunit protein eL24 Homo sapiens 0.017825111 1 LEVO MKVELCSFSGYKIYPGHGRRYARTDGKVFQFLNAKCESAFLSKRNPRQINWTVLYRRKHKKGQSEEIQKKRTRRAVKFQR AITGASLADIMAKRNQKPEVRKAQREQAIRAAKEAKKAKQASKKTAMAAAKAPTKAAPKQKIVKPVKVSAPRVGGKR P83731 Large ribosomal subunit protein uL23 Homo sapiens 0.017740192999999998 1 LEVO MAPKAKKEAPAPPKAEAKAKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFP LTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII P62750 Large ribosomal subunit protein uL24 Homo sapiens 0.017303363 1 LEVO MKFNPFVTSDRSKNRKRHFNAPSHIRRKIMSSPLSKELRQKYNVRSMPIRKDDEVQVVRGHYKGQQIGKVVQVYRKKYVI YIERVQREKANGTTVHVGIHPSKVVITRLKLDKDRKKILERKAKSRQVGKEKGKYKEETIEKMQE P61254 Large ribosomal subunit protein eL27 Homo sapiens 0.015835830999999998 1 LEVO MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHL MPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF P61353 60S ribosomal protein L27a Homo sapiens 0.016604535 1 LEVO MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWT LVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA P46776 Large ribosomal subunit protein eL29 Homo sapiens 0.017817294999999997 1 LEVO MAKS(MLZ)NHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAEAIKALV KPKEVKPKIPKGVSRKLDRLAYIAHPKLGKRARARIAKGLRLCRPKAKAKAKAKDQTKAQAAAPASVPAQAPKRTQAPTK ASE P47914 Large ribosomal subunit protein eL30 Homo sapiens 0.012805092 1 LEVO MVAAKKTKKSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALRKSEIEYYAMLAKTGVHHYSGNNIE LGTACGKYYRVCTLAIIDPGDSDIIRSMPEQTGEK P62888 Large ribosomal subunit protein eL31 Homo sapiens 0.014494937999999999 1 LEVO MAPAKKGGEKKKGRSAINEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTRLNKAVWAKGIRNV PYRIRVRLSRKRNEDEDSPNKLYTLVTYVPVTTFKNLQTVNVDEN P62899 Large ribosomal subunit protein eL32 Homo sapiens 0.015898932 1 LEVO MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVH NVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE P62910 Large ribosomal subunit protein eL33 Homo sapiens 0.012564743 1 LEVO MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGN SGMVRAKFRSNLPAKAIGHRIRVMLYPSRI P18077 Large ribosomal subunit protein eL34 Homo sapiens 0.013326074 1 LEVO MVQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGG SMCAKCVRDRIKRAFLIEEQKIVVKVLKAQAQSQKAK P49207 Large ribosomal subunit protein uL29 Homo sapiens 0.014593624 1 LEVO MAKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKP LDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYAVKA P42766 Large ribosomal subunit protein eL36 Homo sapiens 0.012290859 1 LEVO MALRYPMAVGLNKGHKVTKNVSKPRHSRRRGRLTKHTKFVRDMIREVCGFAPYERRAMELLKVSKDKRALKFIKKRVGTH IRAKRKREELSNVLAAMRKAAAKKD Q9Y3U8 Large ribosomal subunit protein eL37 Homo sapiens 0.011111031999999998 1 LEVO MTKGTSSFGKRRNKTHTLCRRCGSKAYHLQKSTCGKCGYPAKRKRKYNWSAKAKRRNTTGTGRMRHLKIVYRRFRHGFRE GTTPKPKRAAVAASSSS P61927 Large ribosomal subunit protein eL38 Homo sapiens 0.008238948 1 LEVO MPRKIEEIKDFLLTARRKDAKSVKIKKNKDNVKFKVRCSRYLYTLVITDKEKAEKLKQSLPPGLAVKELK P63173 Large ribosomal subunit protein eL39 Homo sapiens 0.006426759 1 LEVO MSSHKTFRIKRFLAKKQKQNRPIPQWIRMKTGNKIRYNSKRRHWRRTKLGL P62891 Ubiquitin-ribosomal protein eL40 fusion protein Homo sapiens 0.014800474 1 LEVO MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEP SLRQLAQKYNCDKMICR(M3L)CYARLHPRAVNCRKKKCGHTNNLRPKKKVK P62987 Small ribosomal subunit protein eS32 Homo sapiens 0.003473451 1 LEVO MRAKWRKKRMRRLKRKRRKMRQRSK P62945 Large ribosomal subunit protein eL42 Homo sapiens 0.012489990999999999 1 LEVO MVNVPKTRRTFCKKCGKHQPHKVTQYKKGKDSLYAQGKRRYDRKQSGYGGQT(MLZ)PIFRKKAKTTKKIVLRLECVEPN CRSKRMLAIKRCKHFELGGDKKRKGQVIQF P83881 Large ribosomal subunit protein eL43 Homo sapiens 0.01029935 1 LEVO MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKTVAGGAWTYNTTSAVTVK SAIRRLKELKDQ P61513 Large ribosomal subunit protein eL28 Homo sapiens 0.015679466 1 LEVO (ACE)SAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATS YVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVMVKRKRTRPTKSS P46779 Nascent chain Homo sapiens 0.0018840489999999998

fusion protein FLAG-VHP-MYC: DYKDDDDKDYKDDDDKDYKDDDDKGSLSDEDFKAVFGMTRSAFANLPLWKQQNLKKEKGLFGSSPQGSPEPLVLHEETPPTTSSDSEEEQEDE

1 Homo sapiens LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)EEQEDE P-site glutamyl-tRNA Homo sapiens 0.024136328 1 UCCCUGGUG(2MG)UC(PSU)AGUGGU(H2U)AGGAUUCGGCGCUCUCACCGCCGCGGCC(5MC)GGGU(2MU)(PSU)G AUUCCCGGUCAGGGAACCA 18S rRNA Homo sapiens 0.603580125 1 UACCUGGUUGAUCCUGCCAGUAGCAU(A2M)UGCUUG(PSU)C(PSU)CAAAGAUUAAGCCAUGCAUGUCUAAGUACGCA CGGCCGGUACAGUGAAACUGCGAA(PSU)GGCUC(A2M)UUAAA(PSU)CAG(PSU)UAUGGU(OMU)CC(PSU)U (OMU)GGUCGCUCGCUCCUCUCCCACUUGGAUAACUGUGGUA(A2M)UUCUAG(A2M)GCUAA(OMU)A(OMC)AUGCCG ACGGGCGCUGACCCCCUUCGCGGGGGGGAUGCGUGCA(PSU)UUAUCAGAUCAAAACCAACCCGGUCAGCCCCUCUCCGG CCCCGGCCGGGGGGCGGGCGCCGGCGGCUUUGGUGACUC(PSU)AGAUAACCUCGGGCCGAUCGCACGCCCCCCGUGGCG GCGACGACCCAUUCGAACGUC(OMU)GCCCUAUCAACUUUCGAUGGUAGUCGCCGUGCCUACCAUGGUGACCACGGG (PSU)GACGGGGAAUCAGGGUUCGAU(OMU)CCGGAGA(OMG)GGAGCCUGAGAAACGGCUACCACAU(OMC)CAAGG (A2M)AGGCAGCAGGCGCGC(A2M)AAUUACCCACUCCCGACCCGGGGA(OMG)GU(A2M)GUGA(OMC)GAAAAAUAAC AAUACAGGACUCUUUCGAGGCCCUGUAAUUGGAAUGAGUCCACU(PSU)(PSU)AA(A2M)UCCUUUAACGAGG(A2M)U CCAUUGGAG(OMG)GCAAGUC(PSU)GGUGCCAGCAGCCGCGG(OMU)AAUUCCAGCUCCAAUA(OMG)CGUA(PSU)A (PSU)UAAAGUUGCUGCAGUU(A2M)AAAAGCUCGUAG(PSU)U(OMG)GA(PSU)CUUGGGAGCGGGCGGGCGGUCCGC CGCGAGGCGAGCCACCGCCCGUCCCCGCCCCUUGCCUCUCGGCGCCCCCUCGAUGCUCUUAGCUGAGUGUCCCGCGGGGC CCGAAGCGUU(PSU)ACUUUGAAAAAA(PSU)(PSU)AGAGUG(PSU)UCAAAGCAGGCCCGAGCCGCCUGGAUACCGCA GCUAGGAA(PSU)AA(PSU)(OMG)GAAUAGGACCGCGGUUCUAUUUUGUUGGUUUUCGGAACUGAGGCCAUGAU(PSU) AAGAGGGACGGCCGGGGGCAUUCGUAUUGCGCCGCUAGAGGUGAAAU(PSU)CUUGGACCGGCGCAAGACGGACCAGAGC GAAAGCAUU(PSU)GCCAAGAAUGUUUUCAUUAAUCAAGA(A2M)CGAAAGUCGGAGGUUCGAAGACGA(PSU)CAGAUA CCGUCGUAGUUCCGACCA(PSU)AAACGAUGCCGACCGGCGAUGCGGCGGCGUUAUUCCCAUGACCCGCCGGGCAGC (PSU)UCCGGGAAACCAAAGUCUUUGGGUUCCGGGGGGAGUA(PSU)GG(PSU)UGCAAAGCUGAAACUUAAAGGAAUUG ACGGAAGGGCACCACCAGGAGUGGAGCC(PSU)GCGGC(PSU)(PSU)AAU(PSU)(PSU)GAC(B8N)CAACACGGGAA ACCUCACCCGGCCCGGACACGGACAGGA(OMU)UGACAGAUUGAUAGCUCUUUCUCGAUUCCGUGGGUGG(UY1)G (OMG)UGCAUGGC(4AC)GUUCUUAGU(PSU)GGUGGAGCGAUUUGUCUGG(PSU)UAAUUCCGAUAACGA(A2M)CGAG ACU(OMC)UGGCAUGCUAACUAGUUACGCGACCCCCGAGCGGUCGGCGUCCCCCAACU(OMU)CU(PSU)A(OMG)AGGG ACAAGUGGCGUUCAGCCACCCGAGAUUGAGCAAUAACA(OMG)GUCUGUGAUGCCCUUAGAUGUCCGGGGCUGCACGCGC GCUACACUGACUGGCUCAGCGUGUGCCUACCCUACGCCGGCAGGCGCGGGUAACCCGUUGAACCCCAUUCGUGAUGGGGA UCGGGGAUUGCAAUUAU(PSU)CCCCAUGAACGAG(G7M)AAU(PSU)CCCAGUAAGUGCGGGUCAUAAGCUUGCGUUGA UU(A2M)AGUCCCUGCCCUU(PSU)GUACACACCG(OMC)CCGUCGCUACUACCGAUUGGAUGGUUUAGUGAGGCCCUCG GAUCGGCCCCGCCGGGGUCGGCCCACGGCCCUGGCGGAGCGCUGAGAAGACGGUCGAACU(OMU)GACUAUCUAGAGGAA GUAAAAGUCGUA(6MZ)CAAGGUUUC(4AC)GUAGGUG(MA6)(MA6)CCUGCGGAAGGAUCAUUA Small ribosomal subunit protein uS2 Homo sapiens 0.032778777 1 LEVO (ACE)SGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSV ISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRY VDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAP EFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS P08865 Small ribosomal subunit protein eS1 Homo sapiens 0.030002061 1 LEVO MAVGKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVA FRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQ VRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGK ATGDETGAKVERADGYEPPVQESV P61247 Small ribosomal subunit protein uS5 Homo sapiens 0.031376516 1 LEVO MADDAGAAGGPGGPGGPGMGNRGGFRGGFGSGIRGRGRGRGRGRGRGRGARGGKAEDKEWMPVTKLGRLVKDMKIKSLEE IYLFSLPIKESEIIDFFLGASLKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAIRGAIILAKL SIVPVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPAPRGTGIVSAPVPKKLLMMAGIDDCYTSARGCTATLGNFAKAT FDAISKTYSYLTPDLWKETVFTKSPYQEFTDHLVKTHTRVSVQRTQAPAVATT P15880 Small ribosomal subunit protein uS3 Homo sapiens 0.026729368999999996 1 LEVO MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFP EGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHS GDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPV PTA P23396 4.2.99.18 Small ribosomal subunit protein eS4, X isoform Homo sapiens 0.029654868999999997 1 LEVO MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDI TYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTI QIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPR GKGIRLTIAEERDKRLAAKQSSG P62701 Small ribosomal subunit protein uS7 Homo sapiens 0.022913453 1 LEVO MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHG RNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGARE AAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR P46782 Small ribosomal subunit protein eS6 Homo sapiens 0.028751905999999997 1 LEVO MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKG HSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRK PLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRAS TSKSESSQK P62753 Small ribosomal subunit protein eS7 Homo sapiens 0.022168914 1 LEVO MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLV RELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDK AQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL P62081 Small ribosomal subunit protein eS8 Homo sapiens 0.024263386999999997 1 LEVO MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIID VVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISS LLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK P62241 Small ribosomal subunit protein uS4 Homo sapiens 0.022641563999999996 1 LEVO MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRR LVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFS LRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED P46781 Small ribosomal subunit protein eS10 Homo sapiens 0.018933846 1 LEVO MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLR DYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGR GQPPQ P46783 Small ribosomal subunit protein uS17 Homo sapiens 0.018468826 1 LEVO MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKM KMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF P62280 Small ribosomal subunit protein eS12 Homo sapiens 0.014538986999999998 1 LEVO MAEEGIAAGGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVKLVEALCAEHQINLIKVD DNKKLGEWVGLCKIDREGKPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKK P25398 Small ribosomal subunit protein uS15 Homo sapiens 0.017259389 1 LEVO MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGL APDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA P62277 Small ribosomal subunit protein uS11 Homo sapiens 0.016302772 1 LEVO MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQD VAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL P62263 Small ribosomal subunit protein uS19 Homo sapiens 0.017076206999999996 1 LEVO MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLNRGLRRKQHSLLKRLRKAKKEAPPMEKPEVVKTHL RDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK P62841 Small ribosomal subunit protein uS9 Homo sapiens 0.016477377 1 LEVO MPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQ IYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR P62249 Small ribosomal subunit protein eS17 Homo sapiens 0.015578156000000001 1 LEVO MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERER RDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGPV P08708 Small ribosomal subunit protein uS13 Homo sapiens 0.017759776999999997 1 LEVO MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIP DWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK P62269 Small ribosomal subunit protein eS19 Homo sapiens 0.016091562 1 LEVO MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYG GRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAAANKKH P39019 Small ribosomal subunit protein uS10 Homo sapiens 0.013398763000000001 1 LEVO MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRF QMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA P60866 40S ribosomal protein S21 Homo sapiens 0.009150427 1 LEVO (ACE)MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKA DGIVSKNF P63220 Small ribosomal subunit protein uS8 Homo sapiens 0.014865554999999999 1 LEVO MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFD VQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF P62244 Small ribosomal subunit protein uS12 Homo sapiens 0.015860666 1 LEVO MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQ(HY3)NSAIRKCVRVQLIK NGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS P62266 40S ribosomal protein S24 Homo sapiens 0.015463333 1 LEVO MNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTTGFGMIYDSLD YAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAGKKPKE P62847 Small ribosomal subunit protein eS25 Homo sapiens 0.013776224 1 LEVO MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIR GSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA P62851 Small ribosomal subunit protein eS26 Homo sapiens 0.013047531999999999 1 LEVO MTKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKKFVIRNIVEAAAVRDISEASVFDAYVLPKLYVKLHYCVSCAIH SKVVRNRSREARKDRTPPPRFRPAGAAPRPPPKPM P62854 Small ribosomal subunit protein eS27 Homo sapiens 0.009480186 1 LEVO MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFR RKQH P42677 Small ribosomal subunit protein eS28 Homo sapiens 0.007855052 1 LEVO MDTSRVQPIKLARVTKVLGRTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLESEREARRLR P62857 Small ribosomal subunit protein uS14 Homo sapiens 0.006690821 1 LEVO MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD P62273 FAU ubiquitin-like and ribosomal protein S30 Homo sapiens 0.014415724 1 LEVO MQLFVRAQELHTFEVTGQETVAQIKAHVASLEGIAPEDQVVLLAGAPLEDEATLGQCGVEALTTLEVAGRMLGGKVHGSL ARAGKVRGQTPKVAKQEKKKKKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS P62861 Ubiquitin-ribosomal protein eS31 fusion protein Homo sapiens 0.018004041000000002 1 LEVO MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKR KKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK P62979 Receptor of activated protein C kinase 1 Homo sapiens 0.035115652 1 LEVO MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALS GSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNS SNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALC FSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR P63244 mRNA Homo sapiens 0.26801184399999994 1 UAAUACGACUCACUAUAGGGCGAAUUAAUUCCGGUUAUUUUCCACCAUAUUGCCGUCUUUUGGCAAUGUGAGGGCCCGGA AACCUGGCCCUGUCUUCUUGACGAGCAUUCCUAGGGGUCUUUCCCCUCUCGCCAAAGGAAUGCAAGGUCUGUUGAAUGUC GUGAAGGAAGCAGUUCCUCUGGAAGCUUCUUGAAGACAAACAACGUCUGUAGCGACCCUUUGCAGGCAGCGGAACCCCCC ACCUGGCGACAGGUGCCUCUGCGGCCAAAAGCCACGUGUAUAAGAUACACCUGCAAAGGCGGCACAACCCCAGUGCCACG UUGUGAGUUGGAUAGUUGUGGAAAGAGUCAAAUGGCUCACCUCAAGCGUAUUCAACAAGGGGCUGAAGGAUGCCCAGAAG GUACCCCAUUGUAUGGGAUCUGAUCUGGGGCCUCGGUGCACAUGCUUUACAUGUGUUUAGUCGAGGUUAAAAAACGUCUA GGCCCCCCGAACCACGGGGACGUGGUUUUCCUUUGAAAAACACGAUGAUAAUAUGAGUGCUUGGAGUCAUCCACAAUUCG AAAAAGGUGGAGGUUCCGGAGGUGGAUCGGGAGGUUCUGCCUGGUCUCAUCCACAAUUUGAGAAGGGAUCACUCUCUGAC GAGGACUUCAAGGCUGUUUUUGGCAUGACCCGCUCUGCCUUUGCCAACUUGCCCUUGUGGAAACAGCAGAACCUCAAGAA GGAGAAAGGACUCUUCGGAUCAUCCCCGCAGGGCAGCCCCGAGCCCCUGGUGCUCCAUGAGGAGACACCGCCCACCACCA GCAGCGACUCUGAGGAGGAACAAGAAGAUGAG POTASSIUM ION 3.9098e-05 170 K 1,4-DIAMINOBUTANE 8.815099999999999e-05 7 PUT SPERMIDINE 0.000145246 14 SPD (2R,3S,4S)-4-hydroxy-2-[(4-methoxyphenyl)methyl]pyrrolidin-3-yl {2-[(2S)-pyrrolidin-2-yl]ethyl}carbamate 0.000363451 1 A1B75 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL 0.00012214299999999999 2 TRS MAGNESIUM ION 2.4305e-05 371 MG ZINC ION 6.5409e-05 8 ZN water 1.8015e-05 7259 HOH singleParticle particle 7.5

20mM Tris, 100mM KOAc, 7.5mM MgOAc, 0.25mM Spermidine, 2mM DTT, 0.05% w/v b-OG, 50uM IDB-001

Quantifoil CARBON HOLEY CARBON CONTINUOUS 2.0 ETHANE 100 292 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.9 2.0 GATAN K3 BIOQUANTUM (6k x 4k) 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 1.9 FSC 0.143 CUT-OFF cryoSPARC 538472 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_70084.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 427.008 427.008 427.008 90.0 90.0 90.0 X Y Z -0.4706198 1.4789988 0.0025411758 0.03358483 0.834 0.834 0.834 0.112 AUTHOR ::::EMDATABANK.org::::EMD-70084:::: Sharpened consensus map emd_70084_msk_1.map emd_70084_additional_3.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 427.008 427.008 427.008 90.0 90.0 90.0 X Y Z -0.098794535 0.499063 0.0025411758 0.017251438 0.834 0.834 0.834 AUTHOR ::::EMDATABANK.org::::EMD-70084:::: Unsharpened consensus map emd_70084_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 427.008 427.008 427.008 90.0 90.0 90.0 X Y Z -0.6277908 1.3682973 0.003028532 0.025633045 0.834 0.834 0.834 AUTHOR ::::EMDATABANK.org::::EMD-70084:::: Sharpened local map of the SSU emd_70084_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 427.008 427.008 427.008 90.0 90.0 90.0 X Y Z -0.12623991 0.5530194 0.002971432 0.01525747 0.834 0.834 0.834 AUTHOR ::::EMDATABANK.org::::EMD-70084:::: Sharpened local map of the LSU emd_70084_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 427.008 427.008 427.008 90.0 90.0 90.0 X Y Z -0.15897512 0.5772934 0.001456845 0.025119048 0.834 0.834 0.834 AUTHOR ::::EMDATABANK.org::::EMD-70084:::: emd_70084_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 427.008 427.008 427.008 90.0 90.0 90.0 X Y Z -0.17115083 0.6066376 0.0014537537 0.025124831 0.834 0.834 0.834 AUTHOR ::::EMDATABANK.org::::EMD-70084::::