<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="https://ftp.ebi.ac.uk/pub/databases/em_ebi/emdb_related/emdb-schemas/emdb_schemas/v3/v3_0_11_0/emdb.xsd" version="3.0.11.0" emdb_id="EMD-70137">
    <admin>
        <current_status>
            <date>2026-04-01</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <revision_history>
            <revision version="1.0" date="2026-02-18">
                <change_list>
                    <metadata>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </metadata>
                    <additional_map part="1">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </additional_map>
                    <fsc>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </fsc>
                    <half_map part="1">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </half_map>
                    <half_map part="2">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </half_map>
                    <image>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </image>
                    <mask part="1">
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </mask>
                    <primary_map>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
                    </primary_map>
                </change_list>
            </revision>
        </revision_history>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-04-10</deposition>
            <header_release>2026-02-18</header_release>
            <map_release>2026-02-18</map_release>
            <update>2026-04-01</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Health and Medical Research Council (NHMRC, Australia)</funding_body>
                <code>APP2036864</code>
                <country>Australia</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Australian Research Council (ARC)</funding_body>
                <code>DE240100992</code>
                <country>Australia</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Other government</funding_body>
                <code>MCRF21036</code>
                <country>Australia</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Australian Research Council (ARC)</funding_body>
                <code>LE170100016</code>
                <country>Australia</country>
            </grant_reference>
        </grant_support>
        <title>KICSTOR-GATOR1 dimer supercomplex (DEPDC5) focused refinement</title>
        <authors_list>
            <author ORCID="0000-0002-7573-7715">Bayly-Jones C</author>
            <author ORCID="0000-0002-4617-4853">Lupton CJ</author>
            <author ORCID="0000-0002-3707-4148">Chang YG</author>
            <author ORCID="0000-0002-2248-9914">Ellisdon AM</author>
        </authors_list>
        <keywords>Lysosome, GATOR1, KICSTOR, cell growth, amino acid sensing, mTOR, KPTN, ITFG2, C12orf66, SZT2, NPRL2, NPRL3, DEPDC5, CELL CYCLE</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Lupton CJ</author>
                    <author order="2">Bayly-Jones C</author>
                    <author order="3">Dong S</author>
                    <author order="4">Lam T</author>
                    <author order="5">Luo W</author>
                    <author order="6">Jones GD</author>
                    <author order="7">Mastos C</author>
                    <author order="8">Frescher NJ</author>
                    <author order="9">Lim SS</author>
                    <author order="10">Keen AC</author>
                    <author order="11">Formosa LE</author>
                    <author order="12">Venugopal H</author>
                    <author order="13">Chang YG</author>
                    <author order="14">Halls ML</author>
                    <author order="15">Ellisdon AM</author>
                    <title>Structure of the lysosomal KICSTOR-GATOR1-SAMTOR nutrient-sensing supercomplex.</title>
                    <journal_abbreviation>Cell</journal_abbreviation>
                    <volume>189</volume>
                    <first_page>1185</first_page>
                    <last_page>1200.e28</last_page>
                    <year>2026</year>
                    <external_references type="PUBMED">41512879</external_references>
                    <external_references type="DOI">doi:10.1016/j.cell.2025.12.005</external_references>
                    <external_references type="ISSN">1097-4172</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-70137</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Dimeric supercomplex of KICSTOR-GATOR1</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Dimeric supercomplex of KICSTOR-GATOR1</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.582</theoretical>
                </molecular_weight>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="2">
                <name>dimeric GATOR1 subcomplex</name>
                <parent>1</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="3">
                <name>SZT2 component of the KICSTOR subcomplex</name>
                <parent>1</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">0.8</concentration>
                    <buffer>
                        <ph>7.4</ph>
                    </buffer>
                    <grid>
                        <model>Quantifoil R1.2/1.3</model>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY</film_topology>
                        </support_film>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <chamber_temperature units="K">278</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS TALOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
                    <nominal_defocus_min units="µm">0.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON III (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">1.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
                </ctf_correction>
                <startup_model type_of_model="OTHER">
                    <details>Ab initio</details>
                </startup_model>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">5.36</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>3861</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="60277">
        <file>emd_70137.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>247</col>
            <row>247</row>
            <sec>247</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>247</x>
            <y>247</y>
            <z>247</z>
        </spacing>
        <cell>
            <a units="Å">247.0</a>
            <b units="Å">247.0</b>
            <c units="Å">247.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-0.1388805</minimum>
            <maximum>11.882529</maximum>
            <average>0.03644474</average>
            <std>0.5728583</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.0</x>
            <y units="Å">1.0</y>
            <z units="Å">1.0</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>3.5</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-70137::::</label>
        <annotation_details>Sharpened final map</annotation_details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <refinement_protocol>FLEXIBLE FIT</refinement_protocol>
            </modelling>
        </modelling_list>
        <segmentation_list>
            <segmentation>
                <file>emd_70137_msk_1.map</file>
            </segmentation>
        </segmentation_list>
        <additional_map_list>
            <additional_map format="CCP4" size_kbytes="15186">
                <file>emd_70137_additional_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>156</col>
                    <row>156</row>
                    <sec>156</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>156</x>
                    <y>156</y>
                    <z>156</z>
                </spacing>
                <cell>
                    <a units="Å">247.69681</a>
                    <b units="Å">247.69681</b>
                    <c units="Å">247.69681</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.04212948</minimum>
                    <maximum>0.13636945</maximum>
                    <average>0.0011875627</average>
                    <std>0.008454802</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.5878</x>
                    <y units="Å">1.5878</y>
                    <z units="Å">1.5878</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-70137::::</label>
            </additional_map>
        </additional_map_list>
        <half_map_list>
            <half_map format="CCP4" size_kbytes="15186">
                <file>emd_70137_half_map_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>156</col>
                    <row>156</row>
                    <sec>156</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>156</x>
                    <y>156</y>
                    <z>156</z>
                </spacing>
                <cell>
                    <a units="Å">247.69681</a>
                    <b units="Å">247.69681</b>
                    <c units="Å">247.69681</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.09462593</minimum>
                    <maximum>0.18562986</maximum>
                    <average>0.0009220117</average>
                    <std>0.013787486</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.5878</x>
                    <y units="Å">1.5878</y>
                    <z units="Å">1.5878</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-70137::::</label>
                <annotation_details>Half map 1</annotation_details>
            </half_map>
            <half_map format="CCP4" size_kbytes="15186">
                <file>emd_70137_half_map_2.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>156</col>
                    <row>156</row>
                    <sec>156</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>156</x>
                    <y>156</y>
                    <z>156</z>
                </spacing>
                <cell>
                    <a units="Å">247.69681</a>
                    <b units="Å">247.69681</b>
                    <c units="Å">247.69681</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.095084995</minimum>
                    <maximum>0.189</maximum>
                    <average>0.00092611084</average>
                    <std>0.013793237</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.5878</x>
                    <y units="Å">1.5878</y>
                    <z units="Å">1.5878</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-70137::::</label>
                <annotation_details>Half map 2</annotation_details>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
