2025-11-12 REL RCSB RCSB RCSB 2025-04-14 2025-11-12 2025-11-12 2025-11-12 National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) UM1 AI144462 United States HIV-1 N332-GT5 SOSIP in complex with mouse polyclonal antibodies (V3-glycan and gp41-base epitopes) following protein multi antigen prime Torres JL Ozorowski G Ward AB EMPEM, BG18, germline targeting, HIV-1, VIRAL PROTEIN Xie Z Wang X Yan Y Steichen JM Ma KM Cottrell CA Melzi E Bottermann M Villavicencio PM Rantalainen K Schiffner T Warner JE Weldon SR Prum T Ellis-Pugh JR Torres JL Jackson AM Flynn CT Ozorowski G Himansu S Carfi A Ward AB Nair U Schief WR Batista FD Simultaneous priming of HIV broadly neutralizing antibody precursors to multiple epitopes by germline-targeting mRNA-LNP immunogens in mouse models. Sci Immunol US 10 eadu7961 eadu7961 2025 41105753 doi:10.1126/sciimmunol.adu7961 2470-9468 EMDB EMD-70192 associated EM volume HIV-1 N332-GT5 SOSIP in complex with mouse polyclonal antibodies (V3-glycan and gp41-base epitopes) following protein multi antigen prime HIV-1 N332-GT5 SOSIP in complex with mouse polyclonal antibodies (V3-glycan and gp41-base epitopes) following protein multi antigen prime 0
Mouse serum IgG isolated and digested to Fab prior to complexing with N332-GT5 antigen
Mus musculus singleParticle particle 0.02 7.4 NEGATIVE uranyl formate
2% uranyl formate, 45 s staining
 Homemade COPPER 400 CARBON CONTINUOUS FEI TECNAI SPIRIT FLOOD BEAM BRIGHT FIELD LAB6 120 1.0 1.5 SIDE ENTRY, EUCENTRIC FEI EAGLE (4k x 4k) 25.0 1 NONE 6V0R
coordinates converted map using UCSF Chimera molmap, and low pass filtered to 40 A
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