(1-methylalkyl)succinate synthase alpha-beta-gamma-delta complex with bound fumarate

2025-08-20 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update RCSB RCSB 2025-04-16 2025-07-23 2025-07-23 2025-08-20 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM126982 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM129882 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM145910 United States (1-methylalkyl)succinate synthase alpha-beta-gamma-delta complex with bound fumarate Andorfer MC Drennan CL glycyl radical enzyme, (1-methylalkyl)succinate synthase, X-succinate synthase, alkylsuccinate synthase, Hydrocarbon degradation, fumarate addition, LYASE Andorfer MC Levitz TS Liu J Chakraborty A King-Roberts DT Nweneka D Imrich CN Drennan CL Structural basis for anaerobic alkane activation by a multisubunit glycyl radical enzyme. Proc.Natl.Acad.Sci.USA US 122 e2510389122 e2510389122 2025 40758891 doi:10.1073/pnas.2510389122 1091-6490 0040 PNASA6 9o8u FULLOVERLAP EMDB EMD-70238 associated EM volume

Heterodimer complex of MASS alpha with beta, gamma, delta subunits Heterodimer complex of MASS alpha with beta, gamma, delta subunits 0 1 2 3 4

one alpha subunits binds beta, gamma, delta subunits, and the other alpha subunit bind only gamma subunits.

Azoarcus sp. HxN1 0.229 1-methyl alkyl succinate synthase subunit MasD Azoarcus sp. HxN1 0.097053289 2 Escherichia coli LEVO MGSSHHHHHHSQMTATSTLSKTDLKNCETVEELRENQQWWWLAERERSARLDYLRKATWKKGALGGNYFDGIRLDLEYPT LFTEAWKKYPNDPSMLRRAKATAYVLDNISIFITDSAQLVGYVGSAPHTIAWRVDGASTVNSEVYNEPGIHAEPEAESLK KVAEINSYWNGQTAVDKVGRLIDPEDAVKFFSGAIGWGTPSSAFGYSGKNFEYFMKGDRAFSQIIAEIDEKIDEAEEATI GTPSPHILPLYDKLNNWHAMKLVLEAAIRFAGRYARLARVMAAKETDEQRKKELLRVAETCERVPANPPRNLQESLQYEH FVQVLARYEAHEGAWPSRPDYYHGPLYAKDVEVEKNITESEAIDLVGEYMIRCSEYGSFSPRYMREGLQGVTGTFVWTLG GVNQDGTDACNGMTIALLKAARLVRVANPTFGFRWHPKVSNEVLRECFECIRQGLGYPTLRNDPVLIQNTMHWYGHPLEE ARTWVHMACMSPNPTTKHGTSPFRMASATMNSAKTIEYVLHNGYDRVVNMQMGPKTGDAREIKDFEDLFERWTVQLKWLM NLLVRTVNLGRFKDPEFFGRPFLSAITERAVEHGIDAVSPEGERGNAWVTAFTWIENVDSMAAIKKLVFDDKKYTMSQLI DALEAEWDGYEQMRLDFVKNGPKWGNDDDYVDDIMLRCLSVAAEHSRNIQCTSGNCWPILPENVSGNIHYANIVGALPNG RRRGDALYDGGVSPGPGLDKAGPTAVLKSVGKIDHVNQGRSFLLNQRLSPTQLAGDKGFQLWNSYVRTWAELGIDHIQFN VISDKVLRAAQNDPEGYQEVIVRVAGYSAHFIDISRKTQDNIIQRTVQGLGENLYFQG A9J4K4 MasB protein Azoarcus sp. HxN1 0.013460091 1 Escherichia coli LEVO MSRRDEWKKLQEEMTRDGGEIKSLETVPEQACGICLNFTDNAYGSDGRGSCNVLKAGSNISLPDVIITRSGENGYITFFN SDAKYCPNFERMKLIDTDGHECADPISRRVQRQLSSIKKS A9J4K0 1-methyl alkyl succinate synthase subunit MasC Azoarcus sp. HxN1 0.0068945529999999994 2 Escherichia coli LEVO STCKECRNYFPINEEASRGDCVRRISDERQSYYTARPTTEAAKCEGCSDYLENTRTAKAH A9J4K2 1-methyl alkyl succinate synthase subunit Mas E Azoarcus sp. HxN1 0.008033104999999999 1 Escherichia coli LEVO MKCTECGHEAEVMKFRYHYNPRIDASLSLRQCPECQAVVTVDELKREVLGRMHNGDDPWGKSAGIENLAEG A9J4K6 FUMARIC ACID 0.000116072 1 FUM 2,3-DIHYDROXY-1,4-DITHIOBUTANE 0.000154251 1 DTT IRON/SULFUR CLUSTER 0.00035163999999999996 3 SF4 FE (III) ION 5.5845e-05 1 FE singleParticle particle 0.8 8.0 50.0 HEPES 300.0 Sodium Chloride 1.0 Fumarate 1.0 DTT

50 mM HEPES pH 8.0, 300 mM NaCl, 1 mM Fumarate, 1 mM DTT

C-flat-1.2/1.3 COPPER 300 CARBON HOLEY GLOW DISCHARGE 45 ETHANE TFS KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.5 GATAN K3 BIOQUANTUM (6k x 4k) 1.42 1 NONE 2.8 FSC 0.143 CUT-OFF RELION 4.0 356678 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_70238.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 299.52 299.52 299.52 90.0 90.0 90.0 X Y Z -0.08370176 0.16106349 0.000095352036 0.002601549 1.17 1.17 1.17 0.0183 AUTHOR ::::EMDATABANK.org::::EMD-70238:::: AlphaFold in silico model AB INITIO MODEL emd_70238_msk_1.map emd_70238_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 299.52 299.52 299.52 90.0 90.0 90.0 X Y Z -0.021616768 0.09172325 0.000009754687 0.0014235858 1.17 1.17 1.17 AUTHOR ::::EMDATABANK.org::::EMD-70238:::: emd_70238_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 299.52 299.52 299.52 90.0 90.0 90.0 X Y Z -0.0235699 0.09235732 0.0000107368005 0.0017548118 1.17 1.17 1.17 AUTHOR ::::EMDATABANK.org::::EMD-70238:::: emd_70238_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 299.52 299.52 299.52 90.0 90.0 90.0 X Y Z -0.023669507 0.09722458 0.000008766079 0.0017515301 1.17 1.17 1.17 AUTHOR ::::EMDATABANK.org::::EMD-70238::::