2026-02-11 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation em_admin _citation.journal_volume _citation.title _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.journal_volume _citation.title _em_admin.last_update RCSB RCSB 2025-04-21 2026-01-28 2026-01-28 2026-02-11 National Science Foundation (NSF, United States) 1817338 United States Sedivy EL Agnello E Song K Xu C Kelch BA bacteriophage, thermophilic, Neck, Portal, VIRUS, VIRUS-DNA complex Sedivy EL Agnello E Hobaugh JE Ahsan R Song K Xu C Kelch BA J.Mol.Biol. UK 438 169641 169641 2026 41544933 doi:10.1016/j.jmb.2026.169641 1089-8638 0070 JMOBAK EMD-70280 consensus EM volume

C1 reconstruction of the thermophilic bacteriophage P74-26 Portal and Portal Vertex

EMD-72288 focused EM volume

dsDNA in the central channel of the bacteriophage P74-26 neck

EMD-70279 focused EM volume

C5 reconstruction of the thermophilic bacteriophage P74-26 Portal Vertex

EMD-70278 focused EM volume

C3 reconstruction of the thermophilic bacteriophage P74-26 Neck

9oae FULLOVERLAP EMDB EMD-70281 associated EM volume

Composite reconstruction of the thermophilic bacteriophage P74-26 Neck and Portal Vertex

EMDB EMD-70280 consensus EM volume

C1 reconstruction of the thermophilic bacteriophage P74-26 Portal and Portal Vertex

EMDB EMD-72288 focused EM volume

dsDNA in the central channel of the bacteriophage P74-26 neck

EMDB EMD-70279 focused EM volume

C5 reconstruction of the thermophilic bacteriophage P74-26 Portal Vertex

EMDB EMD-70278 focused EM volume

C3 reconstruction of the thermophilic bacteriophage P74-26 Neck

Oshimavirus P7426 Oshimavirus P7426 0 1 2 3 4 5 6 7 8 9 10 11 12 Oshimavirus P7426 Thermus thermophilus HB8 HB8 capsid 820.0 7 VIRION SPECIES false false Portal vertex 1 1 2 3 Oshimavirus P7426 Neck 1 4 5 6 7 8 9 10 11 12 Oshimavirus P7426 Major head protein Oshimavirus P7426 0.046680754 20 LEVO MRVPININNALARVRDPLSIGGLKFPTTKEIQEAVAAIADKFNQENDLVDRFFPEDSTFASELELYLLRTQDAEQTGMTF VHQVGSTSLPVEARVAKVDLAKATWSPLAFKESRVWDEKEILYLGRLADEVQAGVINEQIAESLTWLMARMRNRRRWLTW QVMRTGRITIQPNDPYNPNGLKYVIDYGVTDIELPLPQKFDAKDGNGNSAVDPIQYFRDLIKAATYFPDRRPVAIIVGPG FDEVLADNTFVQKYVEYEKGWVVGQNTVQPPREVYRQAALDIFKRYTGLEVMVYDKTYRDQDGSVKYWIPVGELIVLNQS TGPVGRFVYTAHVAGQRNGKVVYATGPYLTVKDHLQDDPPYYAIIAGFHGLPQLSGYNTEDFSFHRFKWLKYANNVQSYL PPFPPKVEL A7XXR6 P74-26 Head Decoration Protein Oshimavirus P7426 0.01637663 20 LEVO MDKIQLFRTIGRVQYWERVPRLHAYGVFALPFPMDPDVEWGNWFAGPHPKAFLVSVHPSGPKAGHVYPTDLSDPDSVANV IGMVLDGHDYEADHNVTVTLRAAVPIEYVQQGIEAPPLQPDPAVLNAAPQLKLKVIKGHYFFDYTR A7XXR5 Portal Vertex Protein Oshimavirus P7426 0.008431445 5 LEVO MRPRRYENPELEQDDLPQPRRKTAYRVYASRRDGKISAWFVVEADSAEEALQLVEQGVYGKGWVPVTAEVLTP A7XXK1 Portal protein Oshimavirus P7426 0.04663446499999999 12 LEVO GPGSIDPSDVPKLEGASVPVMSTSYDVVVDREFDELLQGKDGLLVYHKMLSDGTVKNALNYIFGRIRSAKWYVEPASTDP EDIAIAAFIHAQLGIDDASVGKYPFGRLFAIYENAYIYGMAAGEIVLTLGADGKLILDKIVPIHPFNIDEVLYDEEGGPK ALKLSGEVKGGSQFVSGLEIPIWKTVVFLHNDDGSFTGQSALRAAVPHWLAKRALILLINHGLERFMIGVPTLTIPKSVR QGTKQWEAAKEIVKNFVQKPRHGIILPDDWKFDTVDLKSAMPDAIPYLTYHDAGIARALGIDFNTVQLNMGVQAINIGEF VSLTQQTIISLQREFASAVNLYLIPKLVLPNWPSATRFPRLTFEMEERNDFSAAANLMGMLINAVKDSEDIPTELKALID ALPSKMRRALGVVDEVREAVRQP A7XXR3 Adaptor (P74p90) Oshimavirus P7426 0.014556551 12 LEVO MLPEVSVDEVWYYMPAEVRRPEEVVREGQGGVSLAAFRHIKNGVLAEAASHLKANGVPEGLWNHELIRDYILMQIAARIL RRVRAYQELADSLFADSNIKLRAFLEGVAQVAPDVGTGDWVEDEAILPPF A7XXR8 Tail terminator Oshimavirus P7426 0.017584779 6 LEVO MNPDLKAAHHLKALLENFTWEYPDGEEGRFHQIDVGYFSRPSGYPYAAIHSAKSSAPVVRLGMGAGTLARRFEIELVLTI EYEDPDPQRGYERLTTLRWEVFRHLVLNAQAIPGVEFTDLDEATIEAVTEDDGGFERWGFYGMVLIPIKVVLNPQ A7XXS1 Collar (P74p112) Oshimavirus P7426 0.016145413 24 LEVO MVIDYTITRGTLFVVPASGSVMEVFSPQDGFPLLKLRQENGVFYLKPETTSLLAFSYGHYYVYDENRVLKQRGLLRVQGN LYAPANAQVELLTFRGPGPHQVPALSGQPLLFVNGVLYQDYQLADGVLQVNGVQPEDEVVLVMLGG A7XXU5 Tail Tube Protein gp93 Oshimavirus P7426 0.037955737999999996 6 LEVO MRGVDTFLAFKEQADLKTPATLASLAAGDFLAFNSESLSGRQQVIQSRAIRRMPMRQIAYTANGTVEAGGAVEFTTSNYV LKKLLPLIFHSKTGQEDDPDGDGATFTLVNGGVLTPFTAFVGFDGPEGKYVRRFFGAKVNQATFSARVNDMLNLNLDVQA IGKDILQPGDPGWVNVTPVYPGGDEEYAYVFYQARVLIKAGDMADLAELPVESFDLTINHNLNTNRYRLGSIYRQSLDEG VTEVTGTFTLDAAVKSISGPALNLTGGTAHDPAFLEKVALYGKYAALKLEFIDPTREVAEGVPCRLTIHLPFVRLEEPDF QVRDPGVITGSARFNAYETISVTHVAKF A7XXS2 DNA (31-MER) Oshimavirus P7426 0.00938502 5 (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) DNA DNA (31-MER) Oshimavirus P7426 0.009664453 5 (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) DNA DNA (66-MER) Oshimavirus P7426 0.020091855 1 (DA)(DC)(DC)(DC)(DA)(DC)(DA)(DT)(DC)(DA)(DG)(DC)(DC)(DC)(DA)(DC)(DA)(DC)(DG)(DC) (DA)(DG)(DG)(DG)(DG)(DA)(DG)(DA)(DA)(DA)(DA)(DT)(DT)(DC)(DT)(DG)(DC)(DT)(DG)(DT) (DC)(DC)(DT)(DT)(DC)(DG)(DC)(DC)(DA)(DG)(DT)(DT)(DG)(DC)(DA)(DT)(DC)(DT)(DC)(DT) (DA)(DC)(DC)(DT)(DC)(DC) DNA DNA (66-MER) Oshimavirus P7426 0.020603148 1 (DG)(DG)(DA)(DG)(DG)(DT)(DA)(DG)(DA)(DG)(DA)(DT)(DG)(DC)(DA)(DA)(DC)(DT)(DG)(DG) (DC)(DG)(DA)(DA)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DA)(DG)(DA)(DA)(DT)(DT)(DT)(DT)(DC) (DT)(DC)(DC)(DC)(DC)(DT)(DG)(DC)(DG)(DT)(DG)(DT)(DG)(DG)(DG)(DC)(DT)(DG)(DA)(DT) (DG)(DT)(DG)(DG)(DG)(DT) DNA singleParticle particle 8.0 EMS Lacey Carbon GOLD 400 CARBON LACEY GLOW DISCHARGE 45 AIR ETHANE 90 283 FEI VITROBOT MARK IV

Virions were purified from infected Thermus thermophilus using cesium chloride and sucrose gradient ultracentrifugation

TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.5 1.5 81000.0 NITROGEN GATAN K3 (6k x 4k) 1 16184 49.0571 1 PHASE FLIPPING AND AMPLITUDE CORRECTION FOURIER SPACE 3.4 FSC 0.143 CUT-OFF UCSF ChimeraX used to merge maps into composite

Number of particles used represents the sum of the number of particles in each of the independent maps combined to make this composite. Resolution represents the average resolution of the three independent maps combined to make this composite

90827 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD