<?xml version="1.0" encoding="UTF-8"?>
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    <admin>
        <current_status>
            <date>2025-11-12</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <revision_history>
            <revision version="1.0" date="2025-11-12">
                <change_list>
                    <model>
                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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                    <metadata>
                        <revision_type>INITIAL_RELEASE</revision_type>
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                    </metadata>
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                        <revision_type>INITIAL_RELEASE</revision_type>
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                        <revision_type>INITIAL_RELEASE</revision_type>
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                        <revision_type>INITIAL_RELEASE</revision_type>
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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                </change_list>
            </revision>
        </revision_history>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-04-24</deposition>
            <header_release>2025-11-12</header_release>
            <map_release>2025-11-12</map_release>
            <update>2025-11-12</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>GM116789</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>GM140803</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Science Foundation (NSF, United States)</funding_body>
                <code>1750125</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Sf11 bacteriophage tail</title>
        <authors_list>
            <author>Subramanian S</author>
            <author>Parent KN</author>
        </authors_list>
        <keywords>Sf11, bacteriophage, VIRUS</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Subramanian S</author>
                    <author order="2">Dover JA</author>
                    <author order="3">Parent KN</author>
                    <title>Structure of Shigella bacteriophage Sf11</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>9occ</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-70310</accession_id>
                <content_type>associated EM volume</content_type>
                <details>Sf11 bacteriophage tail</details>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Shigella phage Sf11 SMD-2017</name>
        <supramolecule_list>
            <virus_supramolecule supramolecule_id="1">
                <name>Shigella phage Sf11 SMD-2017</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <sci_species_name ncbi="2282196">Shigella phage Sf11 SMD-2017</sci_species_name>
                <natural_host database="NCBI">
                    <organism ncbi="424720">Shigella flexneri Y</organism>
                </natural_host>
                <virus_type>VIRION</virus_type>
                <virus_isolate>OTHER</virus_isolate>
                <virus_enveloped>false</virus_enveloped>
                <virus_empty>false</virus_empty>
            </virus_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Putative neck whiskers protein gp24</name>
                <natural_source database="NCBI">
                    <organism ncbi="2282196">Shigella phage Sf11 SMD-2017</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.025037666</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MAICANDKGVLVGRMTRLFLAEGCGDAVPDAGDWKYLGSTTSKGVDYSPQTTTSEADTAGGFVSTLVTSSDMTISAEVEI
RKNDPSDEFGFHRLVEIYATELKARRQPSLWVRQVTGATIVTAYCNITSISYEGGTNDIVTGSLEFKVYDSDSVTVESLE
PLAFTTDLQSTGSTGTPLTVAVEGGVAPYTYVWRKDGVVVGSESGATLASPTAGVYTVTVTDSSTDPEVIISTACTVS</string>
                    <external_references type="UNIPROTKB">A0A291AXD7</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Tail fiber protein gp17</name>
                <natural_source database="NCBI">
                    <organism ncbi="2282196">Shigella phage Sf11 SMD-2017</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.050176445</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MAIIKYPDWIPLAQRASKNLTQQTPFRSDQPAVGAPIFQKLTTDIVATWSLTWVFTLAEERAFIQWLRSSRYLNKCNNWF
TMMIDLGGSGLQEQTLHFTDYPVQTSIDGGVVTWTGNVIAKKLNNTMDEFDDVLVELDYRWYGWLDEVVNRELPQVYMTP
ILHMPINMLSSTLDSRIVYDGPQVYYINRNGNLVQSAANEWPLTFIDGVAVGRVPPENTSKNVILYNTNLTNVVWARTRM
SITPSPQTGMLVNGCFKLTPTTDSNTHLVAQLPVFEILEGEVCTFSVIAKADGYRNVRIRIANNAVFVADVTVNLLSGVI
IGGSANSSIENLGGGWFRFSEIVTPTEGGYSAIAMQIWVHDDSNSATFAGDGVSGILVCAPQSDSTGASMSPIATEASPV
TRPTASAKVVMNGATSIDITYSDSSTINVPAVDNYAVIPQADSAWGSKYITTIKFNV</string>
                    <external_references type="UNIPROTKB">A0A291AXC9</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Putative tail protein gp14</name>
                <natural_source database="NCBI">
                    <organism ncbi="2282196">Shigella phage Sf11 SMD-2017</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.092301906</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MPVIHVQKMPGTPKETGIVPAGTNLWKWLNKSNLPASISITVNGRVLGEDDELSFCLRDGDVVNVYCQPSGAIGDLIGAI
LKPVTKIFSFLTPKVSTPKTDTSSKTSPNTSLKAQTNIARNGEARPDNFGQIRAFPDLLQESLFEYINNIKYVTEFMNFG
LGKYDVSSVRYSESNLGSLAGASYTIYQPGEVIPVVYEPYAFDDVDGQELYGPNELDTDPPPVVIETATTTTVTETEFAG
GQIAVKIPKDPAFNYFVDLTMPHDVVFKLNITYAQGGGASVTENVTLSGRLVSATETDDGGLPPANYWYTFIINSINYSG
APISSLSGVTINNTYFNLTDNQPIVSGPYFSPIDGDQLWVHLQHQTNDGNDFSVLIEWWKIDDDNVQIPGTYQSMNYYRD
VDRNDTFYYTIKLTPSAGTGRYAIQMRRTNNSSDTSILQLEEIHSIVTRTNVSYSDDTVVKVVVRATENATGSRDRKYNA
LITRHTIGYNRDAGTVRYTLAPSRSFADAVLHNWLITAGNPENTIDIVRLYEIADSLPDERLGYFDYTFDDEDKSIGERL
QTICDAARVTAFWDDGVMSFSRDEKRDYPATVFNTRNTQSDGYKLSYDISLPGTYDGVNVEYRDPTTNKQANVYYRITDS
GIVEGEPTKAKKFDMLYVRNRYQAVDRAILECRRLIYSRRSMEIKALADGEWVNVGDMIQVVDMYDDVQQTGVIEARNGN
TFTTSEQLTADDNLYVVITSSDGSVSGRLPATVTGLHTFTCNLPSDFQLNIWDGTSVQSESRYVLSTEKELDTTLWVVSQ
KNPGSDGTTTLTMSEYSDEMYEYVISSS</string>
                    <external_references type="UNIPROTKB">A0A291AXC5</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="4">
                <name>gp16</name>
                <natural_source database="NCBI">
                    <organism ncbi="2282196">Shigella phage Sf11 SMD-2017</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.017654083999999997</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MPSLRDYKAKRPNWALFDTITFYHSSFGYVRLVANVLDEMVLGGETYSPVRMDITQSQQSNTPAINATVKFARLANDFKQ
YLKLWTGSGRIEPISALYQRFEETDTNTPLKPYRLYVSDVAMDGSDVTVALSIKNPIKGNVAKLYDIAQFPGLRNV</string>
                    <external_references type="UNIPROTKB">A0A291AXG1</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="5">
                <name>Putative tail protein gp13</name>
                <natural_source database="NCBI">
                    <organism ncbi="2282196">Shigella phage Sf11 SMD-2017</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.061978992</theoretical>
                </molecular_weight>
                <number_of_copies>6</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MATVPTNNPIPSEDPRDLKFNAGKIDEVVNSDAHYYTDRFGKQRWTMAGFQKTTQDAIRSYGYITMDGFEDGATLTLPNQ
VLRYEATGEYYRWDGDFPKTVSSGSTPETAGGVGSGAWLSVGDGSLRADLSSTDDGRGCSLIGTSDGSNVQEALDDINNT
IYQDPLTKWPLGGTLKIKQGVYDVSSALTLDYGNYDATFIGSPGVRAHYVGENMAETIFNCQVADFNLKMLGDTVGATQR
VHAYDYLGNITLKGSATSYGLLVTKKAYTKLENIVSYGHTNGEGLRTDSVLTAELDNVYLQSNRIGWRVLNTGDESELNA
ITVNRLTASQNTQWGILGDRWGAGTTINSLTCEGNGTQGDSGTGGMQIAVNGLNGSCALVLNNPYFEANAGGSDLAIDNT
GTRPVTVIINGGNFHRVSSTAYTVNNLSITSSGGGKVTVILNGTTFQSVGSYVPSSARPYWVTGANCEVIDIGCTFMEQT
SKATSVSSGSIPRSGRINSNGSIDVAPGVSSVNMVTTGVYDVTFSQQLSTSANGYVVQITPISAPDSVSCDVIYSSTTSF
RVTLRNTVSGAGIASAFAFSVTRLV</string>
                    <external_references type="UNIPROTKB">A0A291AXF7</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="6">
                <name>Tail tape measure protein gp18</name>
                <natural_source database="NCBI">
                    <organism ncbi="2282196">Shigella phage Sf11 SMD-2017</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.082248141</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MAEEVGGIVYEVGMDVKGLKAGATTANKTLDDLESSLNKTTGEFNKLDKGARNAGSGMKNAGGAASGLKTSMSMLAGAIS
VSLIIEWGKRFLEVADNMTQLQARIARLSTDAKTANETFSSLANIASTTGASLSDTTKLWETLTSSLKEAGATNAQVLNL
TDTLQKIGRIGGSSTEEMANALRQFGQSIASGTIRAEEFNSILEQMPELARQIAAGLGISMGELRARMLDGKLTAEDALN
AIQDRTSVVNAEFEKLPRTMDQAVGSLEVSFSKLVVAVNDATGASKTAVEIIDQLAKYIDFLGDKSTSTSDKILSLATVI
SKLNPGAWAQMGVDALFGDDDIEKQKQYNDQVERFVKASAEGYEQTKKQAEATKHLKIAQAPTKDKKGKGKGKSAEERQA
ESVAEKLEKLRQQTMLNATSTGELSREQAILNAQQSLGKAATQEQIKLAGEYAAKIWDQKNALKEQAAAEKERIDAVKGY
SALKSKASPMFAVETSYQKDMADLDAYAKAYPQKMAEIQQTRATIEEQYRQQRMDAMWQEWSQQNAATQAAAAAFDAFGQ
TAGNALTGILTGSMSVSEALRSIGSNILSSVINAFVQMGIQWAQSVIMGQAGMAAASAATIAQAAAISAAMAPAAAMTSL
ATAGTNSAPAMAGMSATVGLAKTLSIAGALKNGGPAQEGSMYRVGENNLPEIFQASNGHQYMIPGDSGRVISNKDLTGGG
GGVVVYNNVINNSSAQVSSSARDNGDGSVTIETIISDIENNGPIGQSISRNYSANRRATE</string>
                    <external_references type="UNIPROTKB">A0A291AXC6</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                        <component>
                            <concentration units="mM">10.0</concentration>
                            <name>tris(hydroxymethyl)aminomethane</name>
                        </component>
                        <component>
                            <concentration units="mM">10.0</concentration>
                            <name>magnesium chloride</name>
                        </component>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <chamber_temperature units="K">277.15</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">0.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 BIOCONTINUUM (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">50.45</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <type>NONE</type>
                </ctf_correction>
                <startup_model type_of_model="OTHER"/>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C3</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">3.2</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>35748</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
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