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        <sites>
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            <header_release>2025-12-17</header_release>
            <map_release>2025-12-17</map_release>
            <update>2026-02-25</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</funding_body>
                <code>AI10721-01</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>SIPV3-2E1 Complex</title>
        <authors_list>
            <author>Waddey BT</author>
            <author>Hafenstein SL</author>
        </authors_list>
        <keywords>Virus, Antibody, Complex</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Waddey BT</author>
                    <author order="2">Charnesky AJ</author>
                    <author order="3">Faust JE</author>
                    <author order="4">DiNunno NM</author>
                    <author order="5">Puligedda RD</author>
                    <author order="6">Cho SH</author>
                    <author order="7">Bator CM</author>
                    <author order="8">Dong SD</author>
                    <author order="9">Mahmood K</author>
                    <author order="10">Chumakov KM</author>
                    <author order="11">Dessain SK</author>
                    <author order="12">Hafenstein SL</author>
                    <title>Neutralizing human monoclonal antibodies to poliovirus map to the receptor binding site.</title>
                    <journal_abbreviation>Nat Commun</journal_abbreviation>
                    <country>UK</country>
                    <volume>17</volume>
                    <first_page>1512</first_page>
                    <last_page>1512</last_page>
                    <year>2026</year>
                    <external_references type="PUBMED">41484135</external_references>
                    <external_references type="DOI">doi:10.1038/s41467-025-68226-x</external_references>
                    <external_references type="ISSN">2041-1723</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <pdb_id>9oco</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
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                <db_name>EMDB</db_name>
                <accession_id>EMD-70320</accession_id>
                <content_type>associated EM volume</content_type>
                <details>SIPV3-2E1 Complex</details>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>SIPV3-2E1 Complex</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>SIPV3-2E1 Complex</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="12086">Poliovirus 3</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">8</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>VP1</name>
                <natural_source database="NCBI">
                    <organism ncbi="12086">Poliovirus 3</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.033523672</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>GIEDLISEVAQGALTLSLPKQQDSLPDTKASGPAHSKEVPALTAVETGATNPLAPSDTVQTRHVVQRRSRSESTIESFFA
RGACVAIIEVDNEQPTTRAQKLFAMWRITYKDTVQLRRKLEFFTYSRFDMEFTFVVTANFTNANNGHALNQVYQIMYIPP
GAPTPKSWDDYTWQTSSNPSIFYTYGAAPARISVPYVGLANAYSHFYDGFAKVPLKTDANDQIGDSLYSAMTVDDFGVLA
VRVVNDHNPTKVTSKVRIYMKPKHVRVWCPRPPRAVPYYGPGVDYRNNLDPLSEKGLTTY</string>
                    <external_references type="UNIPROTKB">P03302</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>VP2</name>
                <natural_source database="NCBI">
                    <organism ncbi="12086">Poliovirus 3</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.030169919999999996</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>SPNVEACGYSDRVLQLTLGNSTITTQEAANSVVAYGRWPEFIRDDEANPVDQPTEPDVATCRFYTLDTVMWGKESKGWWW
KLPDALRDMGLFGQNMYYHYLGRSGYTVHVQCNASKFHQGALGVFAIPEYCLAGDSDKQRYTSYANANPGERGGKFYSQF
NKDNAVTSPKREFCPVDYLLGCGVLLGNAFVYPHQIINLRTNNSATIVLPYVNALAIDSMVKHNNWGIAILPLSPLDFAQ
DSSVEIPITVTIAPMCSEFNGLRNVTAPKFQ</string>
                    <external_references type="UNIPROTKB">P03302</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>VP3</name>
                <natural_source database="NCBI">
                    <organism ncbi="12086">Poliovirus 3</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.026289127</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>GLPVLNTPGSNQYLTSDNHQSPCAIPEFDVTPPIDIPGEVKNMMELAEIDTMIPLNLESTKRNTMDMYRVTLSDSADLSQ
PILCLSLSPAFDPRLSHTMLGEVLNYYTHWAGSLKFTFLFCGSMMATGKILVAYAPPGAQPPTSRKEAMLGTHVIWDLGL
QSSCTMVVPWISNVTYRQTTQDSFTEGGYISMFYQTRIVVPLSTPKSMSMLGFVSACNDFSVRLLRDTTHISQSALPQ</string>
                    <external_references type="UNIPROTKB">P03302</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="4">
                <name>VP4</name>
                <natural_source database="NCBI">
                    <organism ncbi="12086">Poliovirus 3</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.007320917</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>GAQVSSQKVGAHENSNRAYGGSTINYTTINYYKDSASNAASKQDYSQDPSKFTEPLKDVLIKTAPALN</string>
                    <external_references type="UNIPROTKB">P03302</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="5">
                <name>2E1 Heavy Chain</name>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.014006592</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>EVQLVQSGAEVKKSGESLKISCKASGYHFTSYWIGWVRQMPGKGLEWMGFIYPGDSDTRYGPSFQGQVSISADKSSSTAY
LQWSRLKASDTAMYFCARHGGFASSLYSSSFRPPDYWGQGTLVTVSS</string>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="6">
                <name>2E1 Light Chain</name>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.011837788</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>NFMLTQPHSVSESPGKTVTISCTRSSGSIASSYVQWYQQRPGSSPTTVIYEDNQRPSGVPDRFSGSIDSSSNSASLIISG
LKTEDEADYYCQSYDSSNQVFGGGTKLTVL</string>
                </sequence>
            </protein_or_peptide>
            <ligand macromolecule_id="7">
                <name>PALMITIC ACID</name>
                <molecular_weight>
                    <theoretical units="MDa">0.00025642399999999994</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <formula>PLM</formula>
            </ligand>
        </macromolecule_list>
    </sample>
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            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>8.0</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">0.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">56.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
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                        <pdb_id>8E8X</pdb_id>
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                    <resolution units="Å" res_type="BY AUTHOR">2.95</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>237897</number_images_used>
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                <initial_angle_assignment>
                    <type>RANDOM ASSIGNMENT</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>RANDOM ASSIGNMENT</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
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        <file>emd_70320.map.gz</file>
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        <dimensions>
            <col>600</col>
            <row>600</row>
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        <pixel_spacing>
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                <level>0.66</level>
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        <label>::::EMDATABANK.org::::EMD-70320::::</label>
        <annotation_details>SIPV3-2E1 Complex map</annotation_details>
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                <pixel_spacing>
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                        <source>AUTHOR</source>
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                <label>::::EMDATABANK.org::::EMD-70320::::</label>
                <annotation_details>Half map 2</annotation_details>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
