2026-03-18 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-05-06 2026-03-18 2026-03-18 2026-03-18 National Science Foundation (NSF, United States) MCB2213840 United States National Science Foundation (NSF, United States) MCB2213841 United States A. agrestis Rubisco (8 RbcL 7 RbcS 1 RbcS-STAR) Ang WSL Oh ZG Li FW Gunn LH Anthoceros agrestis rubisco, pyrenoid, synbio, rubisco linker, PHOTOSYNTHESIS Robison TA Mao Y Oh ZG Ang WSL Loh DH Hsieh YH Ceminsky M Atkinson N Lafferty D Xu X Gunn LH McCormick AJ Li FW An unconventional Rubisco small subunit underpins the CO 2 -concentrating organelle in land plants. Science US 391 1070 1075 2026 41785357 doi:10.1126/science.aea0150 1095-9203 0038 SCIEAS EMD-70517 other EM volume
A. agrestis Rubisco (8 RbcL 8 RbcS)
 EMDB EMD-70517 other EM volume
A. agrestis Rubisco (8 RbcL 8 RbcS)
 EMDB EMD-70520 associated EM volume A. agrestis Rubisco (8 RbcL, 7 RbcS, 1 RbcS-STAR) A. agrestis Rubisco (8 RbcL, 7 RbcS, 1 RbcS-STAR) 0 1 2 3 Anthoceros agrestis 0.55 Rubisco Large Subunit Anthoceros agrestis MSPQTETKAGVGFKAGVKDYRLTYYTPDYETKDTDILAAFRMTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYDIEPVAGEENQYIAYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPAYSKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFVAEAIFKSQAETGEIKGHYLNATAGTCEEMMKRAQFARELGMPIVMHDYLTGGFTANTTLAHYCRDNGLLLHIHRAMHAVIDRQRNHGIHFRVLAKALRMSGGDHIHSGTVVGKLEGEREVTLGFVDLLRDDYIEKDRSRGIYFTQDWVSMPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQARNEGRDLAREGNDIIREASKWSPELAAACEVWKEIKFVFETIDTL other Escherichia coli BL21(DE3) Rubisco Small Subunit Anthoceros agrestis MQVWNPIDNPKFETLSYLPPLTDNQIAREIDYMLRNKWIPCLEFDPSGTITTLPGQPGYYGGRYWTMWKLPMFGCNNAGYVLREIEHCKNAYPGCFIRVLGFDNIRQVQCCAFIVHKPQHHHHHH other Escherichia coli BL21(DE3) Rubisco Small Subunit - SequesTration Associated Region (RbcS-STAR) Anthoceros agrestis MQVWTPVDNKKFETLSYLPALSSNQIAKQIDFMLSKGWIPCLEFDKSAELKVVRGQPGFYNGRYWTMWKLPMFGCNDASSVLREVENCRKAYPNCYIRVLGFDNQRQVQCSAFIVHKPVKNALTEKLDRSLAKPAYKPQPQVSSVVTSRSGWSRDVEVYQPKDDKTVVDKINSLQAGLDGLQRELALLRASAAHDKIKILQGALDGLQREISVLRASVGHTV other Escherichia coli BL21(DE3) singleParticle particle 1 8.0 20.0 C4H11NO3 Tris 50.0 NaCl Sodium chloride
20 mM Tris pH 8.0, 50 mM NaCl
 Quantifoil R1.2/1.3 GOLD 400 CARBON 2.0 GLOW DISCHARGE ETHANE 100 277.15 FEI VITROBOT MARK IV
Blot force 0 for 4 seconds.
FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 0.5 2.5 79000.0 NITROGEN GATAN K3 (6k x 4k) 1 4222 50.0
Images were collected over 40 frames
 1 825654 cryoSPARC 4 Patch CTF estimation PHASE FLIPPING AND AMPLITUDE CORRECTION 9CHZ 1 C1 6.8 FSC 0.143 CUT-OFF cryoSPARC 4 35660 MAXIMUM LIKELIHOOD cryoSPARC 4 MAXIMUM LIKELIHOOD cryoSPARC 4 8 10000.0 cryoSPARC 4 emd_70520.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 279.04 279.04 279.04 90.0 90.0 90.0 X Y Z -0.09771554 0.75752115 0.0051334156 0.022484567 1.09 1.09 1.09 0.0375 AUTHOR A. agrestis Rubisco (8 RbcL 7 RbcS1 1 RbcS-STAR) emd_70520_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 279.04 279.04 279.04 90.0 90.0 90.0 X Y Z -1.3474938 1.4193978 0.0030561774 0.16693416 1.09 1.09 1.09 AUTHOR emd_70520_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 279.04 279.04 279.04 90.0 90.0 90.0 X Y Z -1.5408075 1.3693653 0.002960007 0.16663164 1.09 1.09 1.09 AUTHOR