2026-01-21 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-05-12 2026-01-21 2026-01-21 2026-01-21 Canadian Institutes of Health Research (CIHR) Canada Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Closed Conformation BSol Locally Refined Map Molinarolo SM Van Petegem F Ion channel Calcium Channel, TRANSPORT PROTEIN Molinarolo S Valdivia CR Valdivia HH Van Petegem F Cryo-electron microscopy reveals sequential binding and activation of Ryanodine Receptors by statin triplets. Nat Commun UK 16 11508 11508 2025 41266329 doi:10.1038/s41467-025-66522-0 2041-1723 EMDB EMD-70581 associated EM volume Tetrameric complex of RyR1 with FKBP12.6 Tetrameric complex of RyR1 with FKBP12.6 0 1 2 Oryctolagus cuniculus 2.39 singleParticle particle 10 7.5 10.0 Atorvastatin 2.0 DMSO 2.0 Caffeine 36.0 Free Ca2+ 2.0 EGTA 25.0 Tris 250.0 Sodium Chloride 0.5 TCEP 0.5 Benzamidine 0.375 CHAPS 0.001 DOPC Quantifoil R2/2 COPPER 300 CARBON HOLEY GLOW DISCHARGE AIR 3.9e-05 ETHANE 100 277.15 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM DARK FIELD FIELD EMISSION GUN 300 2.7 0.5 2.5 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN TFS Selectris FEI FALCON IV (4k x 4k) 3 35751 50.0 1 cryoSPARC 4.4.1 PHASE FLIPPING AND AMPLITUDE CORRECTION 7TZC 3.44 FSC 0.143 CUT-OFF cryoSPARC 4.4.1 394024 MAXIMUM LIKELIHOOD cryoSPARC 4.4.1 MAXIMUM LIKELIHOOD cryoSPARC 4.4.1 cryoSPARC 4.4.1 emd_70581.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 491.52 491.52 491.52 90.0 90.0 90.0 X Y Z -0.2530047 0.5340617 0.0011789901 0.011286174 0.96 0.96 0.96 0.146 AUTHOR Structure of Ryanodine Receptor 1: Drug Bound Closed Conformation BSol Locally Refined Map 7TZC A 1-5037 PDB experimental model RIGID BODY FIT REAL emd_70581_msk_1.map emd_70581_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 491.52 491.52 491.52 90.0 90.0 90.0 X Y Z -0.3065449 0.5972905 0.0008715211 0.018573573 0.96 0.96 0.96 AUTHOR Half Map A emd_70581_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 491.52 491.52 491.52 90.0 90.0 90.0 X Y Z -0.3448428 0.58695436 0.0008737826 0.01866377 0.96 0.96 0.96 AUTHOR Half Map B