Cryo-EM structure of Candida albicans fluoride channel FEX in complex with Fab fragment

2026-01-28 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation_author.identifier_ORCID _em_admin.last_update RCSB RCSB 2025-05-16 2025-12-03 2025-12-03 2026-01-28 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM128768 United States Cryo-EM structure of Candida albicans fluoride channel FEX in complex with Fab fragment Kang C-Y An MJ Ohi MD Stockbridge RB Fluoride channel, Fluoride exporter, FEX, MEMBRANE PROTEIN Kang CY An M Heidari S Torabifard H Ohi MD Stockbridge RB The molecular mechanism of fluoride export by the eukaryotic fluoride channel FEX. Nat Commun UK 17 589 589 2025 41381451 doi:10.1038/s41467-025-67289-0 2041-1723 9op1 FULLOVERLAP EMDB EMD-70677 associated EM volume

A complex of FEX with 10E8v4 Fab A complex of FEX with 10E8v4 Fab 0 1 2 3 FEX 1 3 Candida albicans SC5314 10E8v4 Fab 1 1 2 Homo sapiens 10E8v4 Fab Heavy Chian Homo sapiens 0.025050921999999996 1 Cricetulus griseus LEVO EVRLVESGGGLVKPGGSLRLSCSASGFDFDNAWMTWVRQPPGKGLEWVGRITGPGEGWSVDYAESVKGRFTISRDNTKNT LYLEMNNVRTEDTGYYFCARTGKYYDFWSGYPPGEEYFQDWGQGTLVIVSSASTKGPSVFPLAPSSKSTSGGTAALGCLV KDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK 10E8v4 Fab Light Chain Homo sapiens 0.022458944999999998 1 Cricetulus griseus LEVO SELTQDPAVSVALKQTVTITCRGDSLRSHYASWYQKKPGQAPVLLFYGKNNRPSGIPDRFSGSASGNRASLTITGAQAED EADYYCSSRDKSGSRLSVFGGGTKLTVLSQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAG VETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAP Transmembrane protein gp41,Fluoride export protein 1 Candida albicans SC5314 SC5314 0.038810254

C. albicans Fluoride export protein 1, N-terminal epitope tag from HIV Transmembrane protein gp41,C. albicans Fluoride export protein 1, N-terminal epitope tag from HIV Transmembrane protein gp41

1 Saccharomyces cerevisiae LEVO GGLWNWFDITNWLWYIKNLLNIVHGAIWGVLVRKGLMSLTTYSGSFLSGVIWANFAACVVMGLAIDGEVFWIRLLEEKDY PNKGAIPVYTGLTTGFCGTVSSFSSVILEAFNKAADTDIGVRHHYPNGAYGIMQFLAVILAQFGLSIMGFHMGKQFSAVV DNYLPLVTKRIYKVLELTSMILGVVLVVITCILIGVKKQGSWRSWTFSMLFAPFGALLRYYLSKFLNNKVSNFPLGTFTA NFLGTLLLAVFTLLARGKLPGGKGHIVTNTIALHVLEGLDDGFCGGLTTVSTFVVELFGLKTLFSYRYGTISILVCFAGV VLILGSYNWSVGLGGENLYFQSGSAWSHPQFEK P05877 Q5AFH3 singleParticle particle 3.8 7.5 20.0 C8H18N2O4S HEPES 150.0 NaCl Sodium Chloride 5.0 NaF Sodium Fluoride 0.5 C24H46O11 DDM Quantifoil R1.2/1.3 COPPER 300 GLOW DISCHARGE 30 ETHANE 100 291.15 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.8 2.5 105000.0 NITROGEN GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 1.9 50.0 1 cryoSPARC 4.5 PHASE FLIPPING AND AMPLITUDE CORRECTION

AlphaFold2 predicted model for FEX Protein and PDB 5IQ9 for 10E8v4 Fab

C1 4.05 FSC 0.143 CUT-OFF cryoSPARC 4.5 164326 MAXIMUM LIKELIHOOD cryoSPARC 4.5 MAXIMUM LIKELIHOOD cryoSPARC 4.5

non-uniform refinement

emd_70677.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 293.568 293.568 293.568 90.0 90.0 90.0 X Y Z -0.7708014 0.95823455 0.00014806245 0.013944275 0.834 0.834 0.834 0.099 AUTHOR ::::EMDATABANK.org::::EMD-70677:::: c AlphaFold in silico model

Use AlphaFold2 to generate the FEX model.

5IQ9 PDB experimental model emd_70677_msk_1.map emd_70677_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 293.568 293.568 293.568 90.0 90.0 90.0 X Y Z -0.12590629 0.28617567 0.00014806245 0.0077731106 0.834 0.834 0.834 AUTHOR ::::EMDATABANK.org::::EMD-70677:::: emd_70677_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 293.568 293.568 293.568 90.0 90.0 90.0 X Y Z -0.17890581 0.3717517 0.00035636863 0.016105423 0.834 0.834 0.834 AUTHOR ::::EMDATABANK.org::::EMD-70677:::: emd_70677_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 293.568 293.568 293.568 90.0 90.0 90.0 X Y Z -0.19711435 0.40698978 0.0003591392 0.016124625 0.834 0.834 0.834 AUTHOR ::::EMDATABANK.org::::EMD-70677::::