2025-12-24 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation em_admin _citation.journal_volume _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.journal_volume _em_admin.last_update RCSB RCSB 2025-05-26 2025-12-03 2025-12-03 2025-12-24 Canadian Institutes of Health Research (CIHR) PJT-195808 Canada Canadian Institutes of Health Research (CIHR) PJT-168907 Canada Natural Sciences and Engineering Research Council (NSERC, Canada) RGPIN-2018-03951 Canada Natural Sciences and Engineering Research Council (NSERC, Canada) AWD-007855 Canada Cryo-EM structure of the PI4KA complex bound to an EFR3 interfering nanobody (F3IN) Shaw AL Suresh S Yip CK Burke JE PI4KA, TTC7B, FAM126A, Nanobody, Complex, SIGNALING PROTEIN Suresh S Shaw AL Akintola DK Lunke M Doerr S Rohilla P Balla T Yip CK Hansen SD Cobb JA Burke JE Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA. J.Biol.Chem. US 301 110886 110886 2025 41197736 doi:10.1016/j.jbc.2025.110886 1083-351X 0071 JBCHA3 EMD-70822 consensus EM volume
Consensus map of PI4KA/TTC7B/FAM126A/F3IN nanobody
 EMD-70824 focused EM volume
Local Refinement A of PI4KA/TTC7B/FAM126A/F3IN nanobody
 EMD-70825 focused EM volume
Local Refinement B of PI4KA/TTC7B/FAM126A/F3IN nanobody
 9ot6 FULLOVERLAP EMDB EMD-70822 consensus EM volume
Consensus map of PI4KA/TTC7B/FAM126A/F3IN nanobody
 EMDB EMD-70824 focused EM volume
Local Refinement A of PI4KA/TTC7B/FAM126A/F3IN nanobody
 EMDB EMD-70825 focused EM volume
Local Refinement B of PI4KA/TTC7B/FAM126A/F3IN nanobody
 EMDB EMD-70826 associated EM volume
Cryo-EM structure of the PI4KA complex bound to an EFR3 interfering nanobody (F3IN)
 Dimer of heterotetramers of PI4KA,TTC7B,FAM126A, and EFR3 interfering nanobody (F3IN) Dimer of heterotetramers of PI4KA,TTC7B,FAM126A, and EFR3 interfering nanobody (F3IN) 0 1 2 3 4 0.767 PI4KA,TTC7B,FAM126A complex 1 1 3 4 Homo sapiens EFR3 interfering nanobody (F3IN) 1 2 Lama glama Phosphatidylinositol 4-kinase alpha Homo sapiens 0.23724643799999998 2 Spodoptera frugiperda LEVO GSMAAAPARGGGGGGGGGGGCSGSGSSASRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAV IALGIFLIESDLQHKDCVVPYLLRLLKGLPKVYWVEESTARKGRGALPVAESFSFCLVTLLSDVAYRDPSLRDEILEVLL QVLHVLLGMCQALEIQDKEYLCKYAIPCLIGISRAFGRYSNMEESLLSKLFPKIPPHSLRVLEELEGVRRRSFNDFRSIL PSNLLTVCQEGTLKRKTSSVSSISQVSPERGMPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSVSPLFNGVT YKEFNIPLEMLRELLNLVKKIVEEAVLKSLDAIVASVMEANPSADLYYTSFSDPLYLTMFKMLRDTLYYMKDLPTSFVKE IHDFVLEQFNTSQGELQKILHDADRIHNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAH LPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGNDIKISVTNEHSESTLNVMSGKKSQ PSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPIL QILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAA NIQDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDF WLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEV SALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTK ENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAP YRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNT TLGATQLSERPACVKKDYSNFMASLNLRNRYAGEVYGMIRFSGTTGQMSDLNKMMVQDLHSALDRSHPQHYTQAMFKLTA MLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFMREMAGAWHMTVEQKFGLFSAEIKEADPLA ASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLS LLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNL DITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELD QAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHT IDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAS KSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERK KACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEAD GQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYD YFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTD EMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVI QSCFLSNRSRTYDMIQYYQNDIPY P42356 2.7.1.67 EFR3 interfering Nanobody (F3IN) Lama glama 0.018025229 2 Escherichia coli BL21(DE3) LEVO MKYLLPTAAAGLLLLAAQPAMAMAQVQLQESGGGLVQAGGSLRLSCAASGTISASDYMGWYRQAPGKERELVASIDGGGI TNYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAVDWILARYNFVIHYYWGQGTQVTVSSGSYPYDVPDYALEH HHHHH Tetratricopeptide repeat protein 7B Homo sapiens 0.09429410899999999 2 Spodoptera frugiperda LEVO MATKKAGSRLETEIERCRSECQWERIPELVKQLSAKLIANDDMAELLLGESKLEQYLKEHPLRQGASPRGPKPQLTEVRK HLTAALDRGNLKSEFLQESNLIMAKLNYVEGDYKEALNIYARVGLDDLPLTAVPPYRLRVIAEAYATKGLCLEKLPISSS TSNLHVDREQDVITCYEKAGDIALLYLQEIERVILSNIQNRSPKPGPAPHDQELGFFLETGLQRAHVLYFKNGNLTRGVG RFRELLRAVETRTTQNLRMTIARQLAEILLRGMCEQSYWNPLEDPPCQSPLDDPLRKGANTKTYTLTRRARVYSGENIFC PQENTEEALLLLLISESMANRDAVLSRIPEHKSDRLISLQSASVVYDLLTIALGRRGQYEMLSECLERAMKFAFEEFHLW YQFALSLMAAGKSARAVKVLKECIRLKPDDATIPLLAAKLCMGSLHWLEEAEKFAKTVVDVGEKTSEFKAKGYLALGLTY SLQATDASLRGMQEVLQRKALLAFQRAHSLSPTDHQAAFYLALQLAISRQIPEALGYVRQALQLQGDDANSLHLLALLLS AQKHYHDALNIIDMALSEYPENFILLFSKVKLQSLCRGPDEALLTCKHMLQIWKSCYNLTNPSDSGRGSSLLDRTIADRR QLNTITLPDFSDPETGSVHATSVAASRVEQALSEVASSLQSSAPKQGPLHPWMTLAQIWLHAAEVYIGIGKPAEATACTQ EAANLFPMSHNVLYMRGQIAELRGSMDEARRWYEEALAISPTHVKSMQRLALILHQLGRYSLAEKILRDAVQVNSTAHEV WNGLGEVLQAQGNDAAATECFLTALELEASSPAVPFTIIPRVL Q86TV6 Hyccin Homo sapiens 0.034638867 2 Spodoptera frugiperda LEVO MFTSEKGVVEEWLSEFKTLPETSLPNYATNLKDKSSLVSSLYKVIQEPQSELLEPVCHQLFEFYRSGEEQLLQFTLQFLP ELIWCYLAVSASRNVHSSGCIEALLLGVYNLEIVDKQGHTKVLSFTIPSLSKPSVYHEPSSIGSMALTESALSQHGLSKV VYSGPHPQREMLTAQNRFEVLTFLLLCYNAALTYMPSVSLQSLCQICSRICVCGYPRQHVRKYKGISSRIPVSSGFMVQM LTGIYFAFYNGEWDLAQKALDDIIYRAQLELYPEPLLVANAIKASLPHGPMKSNKEGTRCIQVEITPT Q9BYI3 singleParticle particle 0.75 7.0 20.0 C3H4N2 Imidazole 150.0 NaCl Sodium Chloride 5.0 C3H8O3 Glycerol 0.5 Tris(2-carboxyethyl)phosphine C-flat-2/1 COPPER 300 GLOW DISCHARGE AIR
Glow discharged using the Pelco EasiGlow. 15mA Current.
 ETHANE 100 277.15 FEI VITROBOT MARK IV
Blot force -5, blot time 1.5 s..
Gel filtration buffer, filtered through 0.22um filter and degassed. 0.75mg/ml of PI4KA with 3 fold molar excess F3IN Nanobody, with 0.25 mM BS3 crosslinker.
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.5 2.0 165000.0 TFS Selectris FEI FALCON III (4k x 4k) 2 14026 50.0 1 cryoSPARC 4.5.2 PHASE FLIPPING AND AMPLITUDE CORRECTION
PI4KA complex map without F3IN lowpass filtered to 20 Angstroms
 C2 FOURIER SPACE 3.54 FSC 0.143 CUT-OFF cryoSPARC 4.5.2 282982 MAXIMUM LIKELIHOOD cryoSPARC 4.5.2 MAXIMUM LIKELIHOOD cryoSPARC 4.5.2 emd_70826.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 560 560 560 0 0 0 560 560 560 431.19998 431.19998 431.19998 90.0 90.0 90.0 X Y Z -0.034302045 0.21025667 0.0010623881 0.0056027896 0.77 0.77 0.77 0.0371 AUTHOR Composite map 9BAX PDB experimental model
excluding chain C and H
 AlphaFold in silico model
Nanobody F3IN