Avian TRPM8 (Parus major) semi-swapped, ligand-free structure at high pH and 4 degrees Celsius resolved in GDN using cryo-EM

2026-03-25 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-06-25 2026-03-25 2026-03-25 2026-03-25 Howard Hughes Medical Institute (HHMI) United States National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) R35NS105038 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM140847 United States Avian TRPM8 (Parus major) semi-swapped, ligand-free structure at high pH and 4 degrees Celsius resolved in GDN using cryo-EM Choi KY Lin X Cheng Y Julius D TRPM8, transient receptor potential melastatin 8, MEMBRANE PROTEIN Choi KY Lin X Cheng Y Julius D Structural energetics of cold sensitivity Nature UK 2026 doi:10.1038/s41586-026-10276-2 1476-4687 0006 NATUAS 9par FULLOVERLAP EMDB EMD-71444 associated EM volume

Homotetrameric complex of Parus major TRPM8 determined in GDN Homotetrameric complex of Parus major TRPM8 determined in GDN 0 1 Parus major Transient receptor potential cation channel subfamily M member 8 Parus major 0.127217016 4 Homo sapiens LEVO GPGSGSGMRHRRNGNFESSRLLYSSMSRSIDVACSDADLANFIQENFKKRECVFFTKDTKSMGNLCKCGYPENQHIEGTQ VNTTEKWNYKKHTKELPTDAFGDIQFENLGKRGKYIRLSCDTDSETLYDLMTQHWHLKTPNLVISVTGGAKNFALKPRMR KIFSRLIYIAQSKGAWIFTGGTHYGLMKYIGEVVRDNTISRSSEENVVAIGIAAWGMISNRETLIRTADSDGSFLARYIM DDLKRDPLYCLDNNHTHLLLVDNGTHGHPTTEAKVRTQLEKYISERVIPESNYGGKIPIVCFAQGGGKETLKSINVAIKS KIPCVVVEGSGRIADVIASLVEAEGTLASSCVKESLLRFLPRTISRLSEEETESWIKWIKEVLESPHLLTVIKIEEAGDE IVSNAISFALYKAFSTNEHDRDNWNGQLKLLLEWNQLDLASDEIFTNDRNWESADLQDVMFTALVKDRPKFVRLFLENGL NLRKFLTTEVLRELYTNNFSSLVFKNLQIAKNSYNDALLTFVWKMVEDFRRGFKRDYKNSKDEMEIQLSEECPITRHPLQ ALFIWSVLQNKKELSKVIWEQTRGCTLAALGASKLLKSMAKVKNDINAAGESEELANEYETRAVELFTECYSNDEDLAEQ LLTYSCEAWGGSNCLELAVEARDQQFIAQPGVQNFLSKQWYGEISRDTKNWKIIMCLFFFPLIGCGFISFRKKPVEKSKK LFLYYVSFFTSPFVVFSWNVIFYIAFLLLFAYVLLMDFQKEPTALEIILYVLVFVLLCDEVRQWYMNGSKYFSDLWNVMD TLAIFYFIAGIVFRLHSDESSWYSGRVIFCLDYIVFTLRLIHIFTVSRNLGPKIIMLQRMMIDVFFFLFLFAVWMVAFGV ARQGILRKNEHRWEWIFRSVIYEPYLAMFGQYPDDIDGTTYNFDRCTFSGNESKPLCVELDANNQPRFPEWITIPLVCIY MLSTNILLVNLLVAMFGYTVGSVQENNDQVWKFQRFFLVQEYCSRLTIPFPFVIFAYIFMVMRKCFKCCCNKESKEPSIC CSRNEDNEILAWEAVMKENYLVKINTKANDSSEEMVHRFRQLDAKLSDLKGLLKEISSKIK A0A5S8WF66 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate 0.0010470879999999998 4 PT5 singleParticle particle 9.0 300.0 KCl potassium chloride 20.0 C4H11NO3 TRIS 0.021 C56H92O25 glyco-disogenin Quantifoil R1.2/1.3 GOLD CARBON HOLEY ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 1.0 2.5 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 10 GATAN K3 BIOQUANTUM (6k x 4k) 1 15931 2.0 47.7 1 PHASE FLIPPING AND AMPLITUDE CORRECTION

Initial model determined ab-initio and used as an initial reference.

C4 3.0 FSC 0.143 CUT-OFF cryoSPARC 4 289726 MAXIMUM LIKELIHOOD cryoSPARC 4 MAXIMUM LIKELIHOOD cryoSPARC 4 emd_71444.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 262.048 262.048 262.048 90.0 90.0 90.0 X Y Z -2.1194496 3.8440056 0.005217669 0.090060905 0.8189 0.8189 0.8189 0.25 AUTHOR ::::EMDATABANK.org::::EMD-71444:::: emd_71444_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 262.048 262.048 262.048 90.0 90.0 90.0 X Y Z -0.2846679 0.73915803 0.0052176686 0.039917283 0.8189 0.8189 0.8189 AUTHOR ::::EMDATABANK.org::::EMD-71444:::: emd_71444_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 262.048 262.048 262.048 90.0 90.0 90.0 X Y Z -0.30916014 0.753445 0.0027139958 0.04151264 0.8189 0.8189 0.8189 AUTHOR ::::EMDATABANK.org::::EMD-71444:::: emd_71444_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 262.048 262.048 262.048 90.0 90.0 90.0 X Y Z -0.2915876 0.7462121 0.0027138311 0.04152311 0.8189 0.8189 0.8189 AUTHOR ::::EMDATABANK.org::::EMD-71444::::