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    <admin>
        <current_status>
            <date>2026-03-18</date>
            <code>REL</code>
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        </current_status>
        <revision_history>
            <revision version="1.0" date="2026-02-04">
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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                        <revision_type>INITIAL_RELEASE</revision_type>
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-06-26</deposition>
            <header_release>2026-02-04</header_release>
            <map_release>2026-02-04</map_release>
            <update>2026-03-18</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</funding_body>
                <code>1U19AI181881-01</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Negative stain EM map 1 of polyclonal serum from mouse immunized with H5 TX24-FMLMI-foldon in complex with TX24-I53_dn5B.</title>
        <authors_list>
            <author ORCID="0000-0001-6858-4725">Dosey A</author>
            <author ORCID="0000-0002-2978-4692">King NP</author>
        </authors_list>
        <keywords>Fab, Serum, Influenza Hemagglutinin, Immune Complex, IMMUNE SYSTEM</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0000-0001-6858-4725" order="1">Dosey A</author>
                    <author ORCID="0000-0003-0908-6982" order="2">Dadonaite B</author>
                    <author ORCID="0000-0001-5741-3699" order="3">Gillespie RA</author>
                    <author ORCID="0000-0002-5354-6010" order="4">Leaf EM</author>
                    <author ORCID="0000-0002-7110-9623" order="5">Vukovich MJ</author>
                    <author ORCID="0009-0002-6418-3371" order="6">McGowan J</author>
                    <author ORCID="0000-0003-1153-7471" order="7">Grey E</author>
                    <author ORCID="0000-0003-1544-1493" order="8">Muramatsu H</author>
                    <author ORCID="0009-0008-9733-4715" order="9">Jun RHJ</author>
                    <author ORCID="0000-0003-1008-6242" order="10">Pardi N</author>
                    <author ORCID="0000-0001-5767-1532" order="11">Kanekiyo M</author>
                    <author ORCID="0000-0003-1267-3408" order="12">Bloom JD</author>
                    <author ORCID="0000-0002-2978-4692" order="13">King NP</author>
                    <title>Stabilization of the H5 clade 2.3.4.4b hemagglutinin improves vaccine-elicited neutralizing antibody responses in mice.</title>
                    <journal_abbreviation>Sci Transl Med</journal_abbreviation>
                    <country>US</country>
                    <volume>18</volume>
                    <first_page>eaea8770</first_page>
                    <last_page>eaea8770</last_page>
                    <year>2026</year>
                    <external_references type="PUBMED">41779867</external_references>
                    <external_references type="DOI">doi:10.1126/scitranslmed.aea8770</external_references>
                    <external_references type="ISSN">1946-6242</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-71463</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Polyclonal serum from mouse immunized with H5 TX24-FMLMI-foldon in complex with TX24-I53_dn5B.</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Polyclonal serum from mouse immunized with H5 TX24-FMLMI-foldon in complex with TX24-I53_dn5B.</name>
                <parent>0</parent>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="2">
                <name>polyclonal serum from mouse immunized with H5-FMLMI-foldon</name>
                <parent>1</parent>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="3">
                <name>A/Texas/37/2024 HA-I53_dn5B</name>
                <parent>1</parent>
                <natural_source database="NCBI">
                    <organism ncbi="11320">Influenza A virus</organism>
                    <strain>A/Texas/37/2024</strain>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
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        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
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                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <staining>
                        <type>NEGATIVE</type>
                        <material>Uranyl Acetate</material>
                    </staining>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS TALOS L120C</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>LAB6</electron_source>
                    <acceleration_voltage units="kV">120</acceleration_voltage>
                    <nominal_defocus_min units="µm">1.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI CETA (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">40.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
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                <image_recording_id>1</image_recording_id>
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                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
                </ctf_correction>
                <startup_model type_of_model="NONE">
                    <details>Ab initio reconstruction</details>
                </startup_model>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">19.41</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>3269</number_images_used>
                </final_reconstruction>
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                    <type>ANGULAR RECONSTITUTION</type>
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                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>ANGULAR RECONSTITUTION</type>
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                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                </final_angle_assignment>
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        <annotation_details>Negative stain EM map of polyclonal serum from mouse immunized with H5 TX24-FMLMI-foldon in complex with TX24-I53_dn5B.</annotation_details>
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    <interpretation>
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                <label>::::EMDATABANK.org::::EMD-71463::::</label>
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                    <minimum>-2.425873</minimum>
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                <label>::::EMDATABANK.org::::EMD-71463::::</label>
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