2025-12-17 REL RCSB RCSB RCSB 2025-07-20 2025-12-03 2025-12-03 2025-12-17 National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) 1ZIAHL006264 United States Cryo-ET reconstruction of a regenerating axon 24 h after axotomy (primary mouse thalamus neuronal explant) Bodakuntla S Taira K Yamada Y Alvarez-Brecht P Cada AK Basnet N Zhang R Martinez-Sanchez A Biertumpfel C Mizuno N cytoskeleton, microtubules, neuroregeneration, axon, STRUCTURAL PROTEIN Bodakuntla S Taira K Yamada Y Alvarez-Brecht P Cada AK Basnet N Zhang R Martinez-Sanchez A Biertumpfel C Mizuno N In situ structural mechanism of epothilone-B-induced CNS axon regeneration. Nature UK 648 477 487 2025 41224993 doi:10.1038/s41586-025-09654-z 1476-4687 0006 NATUAS EMD-71751 other EM volume
Cryo-ET reconstruction of a regenerating axon after axotomy showing branching microtubules (primary mouse thalamus neuronal explant)
 EMD-71752 other EM volume
Cryo-ET reconstruction of a regenerating axon after axotomy showing polymerizing microtubules (primary mouse thalamus neuronal explant)
 EMD-71753 other EM volume
Cryo-ET reconstruction of a regenerating axon 24 h after axotomy (primary mouse thalamus neuronal explant)
 EMD-71755 other EM volume
Cryo-ET reconstruction of a regenerating axon 24 h after axotomy (primary mouse thalamus neuronal explant)
 EMD-71750 other EM volume
In situ structure of axonal microtubule-shoots at Epothilone B-induced neuroregeneration sites
 PDB 9PND unspecified
In situ structure of axonal microtubule-shoots at Epothilone B-induced neuroregeneration sites
 EMDB EMD-71751 other EM volume
Cryo-ET reconstruction of a regenerating axon after axotomy showing branching microtubules (primary mouse thalamus neuronal explant)
 EMDB EMD-71752 other EM volume
Cryo-ET reconstruction of a regenerating axon after axotomy showing polymerizing microtubules (primary mouse thalamus neuronal explant)
 EMDB EMD-71753 other EM volume
Cryo-ET reconstruction of a regenerating axon 24 h after axotomy (primary mouse thalamus neuronal explant)
 EMDB EMD-71755 other EM volume
Cryo-ET reconstruction of a regenerating axon 24 h after axotomy (primary mouse thalamus neuronal explant)
 EMDB EMD-71754 associated EM volume EMDB EMD-71750 other EM volume
In situ structure of axonal microtubule-shoots at Epothilone B-induced neuroregeneration sites
 Thalamus primary tissue, neuron Thalamus primary tissue, neuron 0
Explant axon from thalamus primary tissue of mouse embryo 24 h after axotomy
Mus musculus Brain Thalamus tomography cell 7.2
Gibco Neurobasal Medium
 Quantifoil R1/4 GOLD 200 SILICON DIOXIDE HOLEY GLOW DISCHARGE AIR 3.8000000000000003
coated with poly-L-lysine and laminin
 ETHANE 95 298 FEI VITROBOT MARK IV No NO SECTIONING TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 2.0 5.0 33000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum GATAN K3 BIOQUANTUM (6k x 4k) 5760 4092 1 61 125.0 1 BACK PROJECTION IMOD 61 CTFPHASEFLIP PHASE FLIPPING AND AMPLITUDE CORRECTION emd_71754.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 1023 1440 464 0 0 0 1023 1440 464 10925.641 15379.2 4955.52 90.0 90.0 90.0 X Y Z -108.573899999999995 62.232500000000002 -0.000000000170608 2.5147274 10.68 10.68 10.68 AUTHOR tomogram