Cryo-EM structure of the human inward-rectifier potassium 7.1 channel (Kir7.1) docked state

2026-04-08 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation_author.identifier_ORCID _em_admin.last_update RCSB RCSB 2025-07-23 2026-02-25 2026-02-25 2026-04-08 Other private United States Cryo-EM structure of the human inward-rectifier potassium 7.1 channel (Kir7.1) docked state Niu Q Vu S Zhang R Fu Z Lishko PV inwardly rectifying potassium channel Kir7.1, KCNJ13, electrophysiology, ion channels, steroids, steroid enantiomers, MEMBRANE PROTEIN Niu Q Vu S Xu Y Qian M Rudenko A Ye J Zeng J Huang W Covey DF Zhang R Fu Z Lishko PV Bioactive lipid-mediated structural and functional regulation of the essential human potassium channel Kir7.1. Nat Commun UK 17 2026 41672996 doi:10.1038/s41467-026-68819-0 2041-1723 9pr6 FULLOVERLAP EMDB EMD-71799 associated EM volume

human inward-rectifier potassium 7.1 channel (Kir7.1) docked state human inward-rectifier potassium 7.1 channel (Kir7.1) docked state 0 1 Homo sapiens 0.16 Inward rectifier potassium channel 13 Homo sapiens 0.040569453 4 Homo sapiens LEVO MDSSNCKVIAPLLSQRYRRMVTKDGHSTLQMDGAQRGLAYLRDAWGILMDMRWRWMMLVFSASFVVHWLVFAVLWYVLAE MNGDLELDHDAPPENHTICVKYITSFTAAFSFSLETQLTIGYGTMFPSGDCPSAIALLAIQMLLGLMLEAFITGAFVAKI ARPKNRAFSIRFTDTAVVAHMDGKPNLIFQVANTRPSPLTSVRVSAVLYQERENGKLYQTSVDFHLDGISSDECPFFIFP LTYYHSITPSSPLATLLQHENPSHFELVVFLSAMQEGTGEICQRRTSYLPSEIMLHHCFASLLTRGSKGEYQIKMENFDK TVPEFPTPLVSKSPNRTDLDIHINGQSIDNFQISETGLTE O60928 [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate 0.000746566 4 PIO singleParticle particle 7.5

20mM Tris-HCl pH 7.5, 150 mM KCl, 0.02% GDN

UltrAuFoil R0./1 GOLD 300 GOLD HOLEY GLOW DISCHARGE 45 AIR

Pelco easiGlow

ETHANE 100 277.15 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.6 2.5 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 BIOQUANTUM (6k x 4k) 5666 50.0 1 2353299 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION

CryoSPARC ab initio

1 C4 FOURIER SPACE 3.9 FSC 0.143 CUT-OFF cryoSPARC 146544 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC cryoSPARC emd_71799.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 238.464 238.464 238.464 90.0 90.0 90.0 X Y Z -0.0113697685 1.8312216 0.0015780225 0.025571346 0.828 0.828 0.828 0.1 AUTHOR ::::EMDATABANK.org::::EMD-71799:::: AlphaFold in silico model BACKBONE TRACE emd_71799_msk_1.map emd_71799_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 238.464 238.464 238.464 90.0 90.0 90.0 X Y Z -0.09476865 0.2687361 0.0022184874 0.011697469 0.828 0.828 0.828 AUTHOR ::::EMDATABANK.org::::EMD-71799:::: emd_71799_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 238.464 238.464 238.464 90.0 90.0 90.0 X Y Z -0.75804853 1.1389972 0.0022184618 0.02784992 0.828 0.828 0.828 AUTHOR ::::EMDATABANK.org::::EMD-71799:::: emd_71799_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 238.464 238.464 238.464 90.0 90.0 90.0 X Y Z -0.104979396 0.27068982 0.0013769092 0.013073207 0.828 0.828 0.828 AUTHOR ::::EMDATABANK.org::::EMD-71799:::: emd_71799_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 238.464 238.464 238.464 90.0 90.0 90.0 X Y Z -0.11029389 0.29482526 0.0013841692 0.012843521 0.828 0.828 0.828 AUTHOR ::::EMDATABANK.org::::EMD-71799::::