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        <current_status>
            <date>2026-04-01</date>
            <code>REL</code>
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        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-07-23</deposition>
            <header_release>2026-01-28</header_release>
            <map_release>2026-01-28</map_release>
            <update>2026-04-01</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Not funded</funding_body>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>HDV particle subtomogram reconstruction</title>
        <authors_list>
            <author ORCID="0000-0003-0705-2359">Itskanov S</author>
            <author ORCID="0000-0001-9461-1475">Lansdon EB</author>
        </authors_list>
        <keywords>HDV, HDAg, RNP, cryo-ET, VIRUS</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0000-0003-0705-2359" order="1">Itskanov S</author>
                    <author order="2">Ary B</author>
                    <author order="3">Mehra U</author>
                    <author ORCID="0000-0003-0153-1236" order="4">Lew I</author>
                    <author order="5">Novikov N</author>
                    <author order="6">Schmitz U</author>
                    <author order="7">Holdorf MM</author>
                    <author ORCID="0000-0001-5435-8014" order="8">Beran RK</author>
                    <author ORCID="0000-0001-9461-1475" order="9">Lansdon EB</author>
                    <title>Structural characterization of the HDV virion and its ribonucleoprotein.</title>
                    <journal_abbreviation>Proc.Natl.Acad.Sci.USA</journal_abbreviation>
                    <country>US</country>
                    <volume>123</volume>
                    <first_page>e2519809123</first_page>
                    <last_page>e2519809123</last_page>
                    <year>2026</year>
                    <external_references type="PUBMED">41564123</external_references>
                    <external_references type="DOI">doi:10.1073/pnas.2519809123</external_references>
                    <external_references type="ISSN">1091-6490</external_references>
                    <external_references type="CSD">0040</external_references>
                    <external_references type="ASTM">PNASA6</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <db_name>EMDB</db_name>
                <accession_id>EMD-71803</accession_id>
                <content_type>associated EM volume</content_type>
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        </other_db_list>
    </crossreferences>
    <sample>
        <name>Hepatitis delta virus</name>
        <supramolecule_list>
            <virus_supramolecule supramolecule_id="1">
                <name>Hepatitis delta virus</name>
                <parent>0</parent>
                <sci_species_name ncbi="12475">Hepatitis delta virus</sci_species_name>
                <natural_host database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_host>
                <virus_shell shell_id="1">
                    <diameter units="Å">350.0</diameter>
                </virus_shell>
                <virus_type>VIRION</virus_type>
                <virus_isolate>STRAIN</virus_isolate>
                <virus_enveloped>true</virus_enveloped>
                <virus_empty>false</virus_empty>
            </virus_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>subtomogramAveraging</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <subtomogram_averaging_preparation preparation_id="1">
                    <buffer>
                        <ph>8.0</ph>
                        <details>25mM Tris-HCl pH 8.0, 150mM NaCl, 1mM EDTA</details>
                    </buffer>
                    <grid>
                        <model>Quantifoil R1.2/1.3</model>
                        <material>COPPER</material>
                        <mesh>300</mesh>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                            <time units="s">45</time>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <chamber_temperature units="K">10</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                </subtomogram_averaging_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <subtomogram_averaging_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">1.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">4.0</nominal_defocus_max>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <coma_free/>
                    </alignment_procedure>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON IV (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">1.6</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </subtomogram_averaging_microscopy>
            </microscopy_list>
            <subtomogram_averaging_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C1</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">36.0</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>EMAN2</name>
                        </software>
                    </software_list>
                    <number_subtomograms_used>358</number_subtomograms_used>
                </final_reconstruction>
                <extraction>
                    <number_tomograms>912</number_tomograms>
                    <number_images_used>358</number_images_used>
                    <software_list>
                        <software>
                            <name>EMAN2</name>
                        </software>
                    </software_list>
                </extraction>
                <ctf_correction>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
                </ctf_correction>
                <final_three_d_classification>
                    <number_classes>1</number_classes>
                </final_three_d_classification>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
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                        <software>
                            <name>EMAN2</name>
                        </software>
                    </software_list>
                </final_angle_assignment>
            </subtomogram_averaging_processing>
        </structure_determination>
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            <space_group>1</space_group>
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            <sec>512</sec>
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            <z>512</z>
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            <b units="Å">474.624</b>
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            <beta units="deg">90.0</beta>
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                <source>AUTHOR</source>
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        <label>::::EMDATABANK.org::::EMD-71803::::</label>
        <annotation_details>HDV particle subtomogram reconstruction. Unsharpened.</annotation_details>
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    <interpretation>
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                    <z>512</z>
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                    <medium>Y</medium>
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                        <source>AUTHOR</source>
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                </contour_list>
                <label>::::EMDATABANK.org::::EMD-71803::::</label>
                <annotation_details>HDV particle subtomogram reconstruction. Half map - even.</annotation_details>
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                    <row>512</row>
                    <sec>512</sec>
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                    <c units="Å">474.624</c>
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                <label>::::EMDATABANK.org::::EMD-71803::::</label>
                <annotation_details>HDV particle subtomogram reconstruction. Half map - odd.</annotation_details>
            </half_map>
        </half_map_list>
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