data_EMD-71806
# 
_entry.id   EMD-71806 
# 
_audit_conform.dict_name       mmcif_pdbx.dic 
_audit_conform.dict_version    5.412 
_audit_conform.dict_location   http://mmcif.pdb.org/dictionaries/ascii/mmcif_pdbx.dic 
# 
loop_
_database_2.database_id 
_database_2.database_code 
_database_2.pdbx_database_accession 
_database_2.pdbx_DOI 
WWPDB D_1000295526 ? ? 
EMDB  EMD-71806    ? ? 
# 
loop_
_pdbx_audit_revision_history.ordinal 
_pdbx_audit_revision_history.data_content_type 
_pdbx_audit_revision_history.major_revision 
_pdbx_audit_revision_history.minor_revision 
_pdbx_audit_revision_history.revision_date 
_pdbx_audit_revision_history.part_number 
1 'EM metadata' 1 0 2026-01-28 ? 
2 FSC           1 0 2026-01-28 ? 
3 'Half map'    1 0 2026-01-28 1 
4 'Half map'    1 0 2026-01-28 2 
5 Image         1 0 2026-01-28 ? 
6 'Primary map' 1 0 2026-01-28 ? 
# 
loop_
_pdbx_audit_revision_details.ordinal 
_pdbx_audit_revision_details.revision_ordinal 
_pdbx_audit_revision_details.data_content_type 
_pdbx_audit_revision_details.provider 
_pdbx_audit_revision_details.type 
_pdbx_audit_revision_details.description 
_pdbx_audit_revision_details.details 
1 1 'EM metadata' repository 'Initial release' ? ? 
2 2 FSC           repository 'Initial release' ? ? 
3 3 'Half map'    repository 'Initial release' ? ? 
4 4 'Half map'    repository 'Initial release' ? ? 
5 5 Image         repository 'Initial release' ? ? 
6 6 'Primary map' repository 'Initial release' ? ? 
# 
loop_
_pdbx_database_related.db_name 
_pdbx_database_related.details 
_pdbx_database_related.db_id 
_pdbx_database_related.content_type 
EMDB . EMD-71805 'consensus EM volume'  
EMDB . EMD-71806 'associated EM volume' 
# 
_citation.abstract                  ? 
_citation.abstract_id_CAS           ? 
_citation.book_id_ISBN              ? 
_citation.book_publisher            ? 
_citation.book_publisher_city       ? 
_citation.book_title                ? 
_citation.coordinate_linkage        ? 
_citation.country                   US 
_citation.database_id_Medline       ? 
_citation.details                   ? 
_citation.id                        primary 
_citation.journal_abbrev            Proc.Natl.Acad.Sci.USA 
_citation.journal_id_ASTM           PNASA6 
_citation.journal_id_CSD            0040 
_citation.journal_id_ISSN           1091-6490 
_citation.journal_full              ? 
_citation.journal_issue             ? 
_citation.journal_volume            123 
_citation.language                  ? 
_citation.page_first                e2519809123 
_citation.page_last                 e2519809123 
_citation.title                     'Structural characterization of the HDV virion and its ribonucleoprotein.' 
_citation.year                      2026 
_citation.database_id_CSD           ? 
_citation.pdbx_database_id_DOI      10.1073/pnas.2519809123 
_citation.pdbx_database_id_PubMed   41564123 
_citation.pdbx_database_id_patent   ? 
_citation.unpublished_flag          ? 
# 
loop_
_citation_author.citation_id 
_citation_author.name 
_citation_author.ordinal 
_citation_author.identifier_ORCID 
primary 'Itskanov, S.'  1 0000-0003-0705-2359 
primary 'Ary, B.'       2 ?                   
primary 'Mehra, U.'     3 ?                   
primary 'Lew, I.'       4 0000-0003-0153-1236 
primary 'Novikov, N.'   5 ?                   
primary 'Schmitz, U.'   6 ?                   
primary 'Holdorf, M.M.' 7 ?                   
primary 'Beran, R.K.'   8 0000-0001-5435-8014 
primary 'Lansdon, E.B.' 9 0000-0001-9461-1475 
# 
loop_
_entity.details 
_entity.formula_weight 
_entity.id 
_entity.src_method 
_entity.type 
_entity.pdbx_description 
_entity.pdbx_number_of_molecules 
_entity.pdbx_parent_entity_id 
_entity.pdbx_mutation 
_entity.pdbx_fragment 
_entity.pdbx_ec 
_entity.pdbx_modification 
_entity.pdbx_formula_weight_exptl 
_entity.pdbx_formula_weight_exptl_method 
_entity.pdbx_target_id 
_entity.pdbx_entities_per_biological_unit 
? ? 1 man polymer 'HDV delta antigen, HDAg'                  ? ? ? ? ? ? ? ? ? ? 
;311 nucleotides derived from HDV antigenomic RNA. Residues from the T7 promoter (GGGAGA) and HindIII restriction site (AAGCU) are added to start and end of sequence, respectively.
;
? 2 syn polymer 'Truncated HDV antigenomic RNA, 311-agRNA' ? ? ? ? ? ? ? ? ? ? 
# 
loop_
_entity_poly.entity_id 
_entity_poly.type 
_entity_poly.pdbx_seq_one_letter_code 
1 'polypeptide(L)'   
;SRPEGRKNRGGREEVLEQWVSGRKKLEELERDLRKVKKKIKKLEDEHPWLGNIKGILGKKDKDGEGAPPAKRARTDQMEVDSGPRKRPSRGGFTDKERQDHRRRKALENKRKQLSAGGKNLSKEEEEELRRLTEEDERRERRIAGPQVGGVNPLEGGTRGAPGGGFVPSMQGVPESPFTRTGEGLDIRGSQGFP
;
2 polyribonucleotide 
;GGGAGAUUUUUCCUUCUUGGGAGUUGUAUCUCCGACGUUCCAAUGCUCUUUACCGACUCACCCCUCUCGGGCGCUGAUCUAUCCUCCCCGCGAAUCCUUGUUCGGAACUUGGCUCAUCUCGAACUUGGGCGACAGGGCCAGCAGUCUCCUCUUUACAGAAAAGAGUAAGAGUACUGAGGACCGUCGCCUCUAGUCGAAGAUGAGCCGGCCCGAAGGAAGGAAAAACCGCGGGGGGAGAGAAGAGGUUCUCGAGCAGUGGGUGAGCGGAAGAAAGAAGUUAGAGGAACUCGAGAGAGACCUCCGGAAGGUUAAGAAGAAAGCU
;
# 
_entity_src_gen.entity_id                          1 
_entity_src_gen.gene_src_common_name               ? 
_entity_src_gen.gene_src_details                   ? 
_entity_src_gen.gene_src_genus                     ? 
_entity_src_gen.gene_src_species                   ? 
_entity_src_gen.gene_src_strain                    ? 
_entity_src_gen.gene_src_tissue                    ? 
_entity_src_gen.gene_src_tissue_fraction           ? 
_entity_src_gen.host_org_genus                     ? 
_entity_src_gen.host_org_species                   ? 
_entity_src_gen.pdbx_gene_src_fragment             ? 
_entity_src_gen.pdbx_gene_src_gene                 ? 
_entity_src_gen.pdbx_gene_src_scientific_name      'Hepatitis delta virus' 
_entity_src_gen.pdbx_gene_src_variant              ? 
_entity_src_gen.pdbx_gene_src_cell_line            ? 
_entity_src_gen.pdbx_gene_src_atcc                 ? 
_entity_src_gen.pdbx_gene_src_organ                ? 
_entity_src_gen.pdbx_gene_src_organelle            ? 
_entity_src_gen.pdbx_gene_src_plasmid              ? 
_entity_src_gen.pdbx_gene_src_plasmid_name         ? 
_entity_src_gen.pdbx_gene_src_cell                 ? 
_entity_src_gen.pdbx_gene_src_cellular_location    ? 
_entity_src_gen.pdbx_host_org_gene                 ? 
_entity_src_gen.pdbx_host_org_organ                ? 
_entity_src_gen.pdbx_host_org_organelle            ? 
_entity_src_gen.pdbx_host_org_cellular_location    ? 
_entity_src_gen.pdbx_host_org_strain               ? 
_entity_src_gen.pdbx_host_org_tissue_fraction      ? 
_entity_src_gen.pdbx_description                   ? 
_entity_src_gen.host_org_common_name               ? 
_entity_src_gen.host_org_details                   ? 
_entity_src_gen.host_org_strain                    ? 
_entity_src_gen.plasmid_details                    ? 
_entity_src_gen.plasmid_name                       ? 
_entity_src_gen.pdbx_host_org_variant              ? 
_entity_src_gen.pdbx_host_org_cell_line            ? 
_entity_src_gen.pdbx_host_org_atcc                 ? 
_entity_src_gen.pdbx_host_org_culture_collection   ? 
_entity_src_gen.pdbx_host_org_cell                 ? 
_entity_src_gen.pdbx_host_org_scientific_name      'Escherichia coli' 
_entity_src_gen.pdbx_host_org_tissue               ? 
_entity_src_gen.pdbx_host_org_vector               ? 
_entity_src_gen.pdbx_host_org_vector_type          ? 
_entity_src_gen.expression_system_id               ? 
_entity_src_gen.gene_src_dev_stage                 ? 
_entity_src_gen.start_construct_id                 ? 
_entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id     12475 
_entity_src_gen.pdbx_host_org_ncbi_taxonomy_id     562 
_entity_src_gen.pdbx_src_id                        1 
_entity_src_gen.pdbx_alt_source_flag               ? 
_entity_src_gen.pdbx_seq_type                      ? 
_entity_src_gen.pdbx_beg_seq_num                   ? 
_entity_src_gen.pdbx_end_seq_num                   ? 
_entity_src_gen.pdbx_gene_src_culture_collection   ? 
# 
_pdbx_entity_src_syn.details                ? 
_pdbx_entity_src_syn.organism_scientific    'Hepatitis delta virus' 
_pdbx_entity_src_syn.organism_common_name   ? 
_pdbx_entity_src_syn.strain                 ? 
_pdbx_entity_src_syn.ncbi_taxonomy_id       12475 
_pdbx_entity_src_syn.entity_id              2 
_pdbx_entity_src_syn.pdbx_src_id            1 
_pdbx_entity_src_syn.pdbx_alt_source_flag   ? 
_pdbx_entity_src_syn.pdbx_beg_seq_num       ? 
_pdbx_entity_src_syn.pdbx_end_seq_num       ? 
# 
_exptl.entry_id         EMD-71806 
_exptl.details          ? 
_exptl.method           'ELECTRON MICROSCOPY' 
_exptl.method_details   ? 
# 
_struct_keywords.entry_id        EMD-71806 
_struct_keywords.text            'HDV, HDAg, RNP, RNA, VIRUS' 
_struct_keywords.pdbx_keywords   VIRUS 
_struct_keywords.pdbx_details    ? 
# 
_em_3d_fitting.id                1 
_em_3d_fitting.entry_id          EMD-71806 
_em_3d_fitting.method            ? 
_em_3d_fitting.target_criteria   ? 
_em_3d_fitting.details           ? 
_em_3d_fitting.overall_b_value   ? 
_em_3d_fitting.ref_space         ? 
_em_3d_fitting.ref_protocol      ? 
# 
_em_3d_reconstruction.entry_id                    EMD-71806 
_em_3d_reconstruction.id                          1 
_em_3d_reconstruction.method                      ? 
_em_3d_reconstruction.algorithm                   'FOURIER SPACE' 
_em_3d_reconstruction.citation_id                 ? 
_em_3d_reconstruction.details                     'Multibody refinement from RELION' 
_em_3d_reconstruction.resolution                  7.3 
_em_3d_reconstruction.resolution_method           'FSC 0.143 CUT-OFF' 
_em_3d_reconstruction.magnification_calibration   ? 
_em_3d_reconstruction.nominal_pixel_size          ? 
_em_3d_reconstruction.actual_pixel_size           ? 
_em_3d_reconstruction.num_particles               43959 
_em_3d_reconstruction.euler_angles_details        ? 
_em_3d_reconstruction.num_class_averages          ? 
_em_3d_reconstruction.fsc_type                    'EVEN/ODD MAPS REFINED TOTALLY INDEPENDENT (GOLD STANDARD)' 
_em_3d_reconstruction.refinement_type             ? 
_em_3d_reconstruction.image_processing_id         1 
_em_3d_reconstruction.symmetry_type               POINT 
# 
_em_buffer.id            1 
_em_buffer.specimen_id   1 
_em_buffer.name          ? 
_em_buffer.details       '20mM Tris-HCl, 200mM NaCl' 
_em_buffer.pH            8.0 
# 
loop_
_em_entity_assembly.id 
_em_entity_assembly.parent_id 
_em_entity_assembly.source 
_em_entity_assembly.type 
_em_entity_assembly.name 
_em_entity_assembly.details 
_em_entity_assembly.go_id 
_em_entity_assembly.ipr_id 
_em_entity_assembly.synonym 
_em_entity_assembly.number_of_copies 
_em_entity_assembly.oligomeric_details 
_em_entity_assembly.entity_id_list 
_em_entity_assembly.mutant_flag 
_em_entity_assembly.chimera 
1 0 'MULTIPLE SOURCES' COMPLEX 'Twisted arrangement map of S-HDAg bound to 311-agRNA' ? ? ? ? ? ? '1, 2' ? NO 
2 1 RECOMBINANT        COMPLEX S-HDAg                                                 ? ? ? ? ? ? 1      ? NO 
3 1 SYNTHETIC          COMPLEX 311-agRNA                                              ? ? ? ? ? ? 2      ? NO 
# 
_em_imaging.entry_id                        EMD-71806 
_em_imaging.id                              1 
_em_imaging.astigmatism                     ? 
_em_imaging.electron_beam_tilt_params       ? 
_em_imaging.residual_tilt                   ? 
_em_imaging.sample_support_id               1 
_em_imaging.detector_id                     1 
_em_imaging.scans_id                        1 
_em_imaging.microscope_id                   ? 
_em_imaging.microscope_model                'TFS KRIOS' 
_em_imaging.specimen_holder_type            ? 
_em_imaging.specimen_holder_model           ? 
_em_imaging.details                         ? 
_em_imaging.date                            ? 
_em_imaging.accelerating_voltage            300 
_em_imaging.illumination_mode               'FLOOD BEAM' 
_em_imaging.mode                            'BRIGHT FIELD' 
_em_imaging.nominal_cs                      ? 
_em_imaging.nominal_defocus_min             720 
_em_imaging.nominal_defocus_max             2500 
_em_imaging.calibrated_defocus_min          ? 
_em_imaging.calibrated_defocus_max          ? 
_em_imaging.tilt_angle_min                  ? 
_em_imaging.tilt_angle_max                  ? 
_em_imaging.nominal_magnification           ? 
_em_imaging.calibrated_magnification        ? 
_em_imaging.electron_source                 'FIELD EMISSION GUN' 
_em_imaging.citation_id                     ? 
_em_imaging.temperature                     ? 
_em_imaging.detector_distance               ? 
_em_imaging.recording_temperature_minimum   ? 
_em_imaging.recording_temperature_maximum   ? 
_em_imaging.alignment_procedure             'COMA FREE' 
_em_imaging.c2_aperture_diameter            ? 
_em_imaging.specimen_id                     1 
_em_imaging.cryogen                         NITROGEN 
_em_imaging.objective_aperture              ? 
_em_imaging.microscope_serial_number        ? 
_em_imaging.microscope_version              ? 
# 
_em_sample_support.id               1 
_em_sample_support.film_material    ? 
_em_sample_support.method           ? 
_em_sample_support.grid_material    GOLD 
_em_sample_support.grid_mesh_size   300 
_em_sample_support.grid_type        'UltrAuFoil R1.2/1.3' 
_em_sample_support.pretreatment     ? 
_em_sample_support.details          ? 
_em_sample_support.specimen_id      1 
_em_sample_support.citation_id      ? 
# 
_em_vitrification.entry_id                EMD-71806 
_em_vitrification.id                      1 
_em_vitrification.sample_preparation_id   ? 
_em_vitrification.specimen_id             1 
_em_vitrification.cryogen_name            ETHANE 
_em_vitrification.humidity                100 
_em_vitrification.temp                    ? 
_em_vitrification.chamber_temperature     283 
_em_vitrification.instrument              'FEI VITROBOT MARK IV' 
_em_vitrification.method                  ? 
_em_vitrification.time_resolved_state     ? 
_em_vitrification.citation_id             ? 
_em_vitrification.details                 ? 
# 
_em_experiment.entry_id                EMD-71806 
_em_experiment.id                      1 
_em_experiment.reconstruction_method   'SINGLE PARTICLE' 
_em_experiment.aggregation_state       PARTICLE 
_em_experiment.entity_assembly_id      1 
# 
_em_single_particle_entity.entry_id              EMD-71806 
_em_single_particle_entity.id                    1 
_em_single_particle_entity.image_processing_id   1 
_em_single_particle_entity.point_symmetry        C1 
# 
_em_admin.current_status        REL 
_em_admin.deposition_date       2025-07-23 
_em_admin.deposition_site       RCSB 
_em_admin.details               ? 
_em_admin.entry_id              EMD-71806 
_em_admin.last_update           2026-04-01 
_em_admin.map_release_date      2026-01-28 
_em_admin.header_release_date   2026-01-28 
_em_admin.obsoleted_date        ? 
_em_admin.title                 'Twisted arrangement of the HDV 311-RNP, Multibody refinement, Body 1' 
_em_admin.process_site          RCSB 
_em_admin.composite_map         NO 
# 
loop_
_em_author_list.author 
_em_author_list.identifier_ORCID 
_em_author_list.ordinal 
'Itskanov, S.'  0000-0003-0705-2359 1 
'Lansdon, E.B.' 0000-0001-9461-1475 2 
# 
loop_
_em_buffer_component.buffer_id 
_em_buffer_component.concentration 
_em_buffer_component.concentration_units 
_em_buffer_component.formula 
_em_buffer_component.id 
_em_buffer_component.name 
1 20  mM ?    1 'Tris(hydroxymethyl)aminomethane hydrochloride' 
1 200 mM NaCl 2 'sodium chloride'                               
# 
_em_ctf_correction.amplitude_correction              ? 
_em_ctf_correction.amplitude_correction_factor       ? 
_em_ctf_correction.amplitude_correction_space        ? 
_em_ctf_correction.correction_operation              ? 
_em_ctf_correction.details                           ? 
_em_ctf_correction.em_image_processing_id            1 
_em_ctf_correction.id                                1 
_em_ctf_correction.phase_reversal                    ? 
_em_ctf_correction.phase_reversal_anisotropic        ? 
_em_ctf_correction.phase_reversal_correction_space   ? 
_em_ctf_correction.type                              'PHASE FLIPPING AND AMPLITUDE CORRECTION' 
# 
_em_entity_assembly_molwt.entity_assembly_id   1 
_em_entity_assembly_molwt.experimental_flag    NO 
_em_entity_assembly_molwt.id                   1 
_em_entity_assembly_molwt.units                ? 
_em_entity_assembly_molwt.value                ? 
_em_entity_assembly_molwt.method               ? 
# 
loop_
_em_entity_assembly_naturalsource.cell 
_em_entity_assembly_naturalsource.cellular_location 
_em_entity_assembly_naturalsource.details 
_em_entity_assembly_naturalsource.entity_assembly_id 
_em_entity_assembly_naturalsource.id 
_em_entity_assembly_naturalsource.ncbi_tax_id 
_em_entity_assembly_naturalsource.organ 
_em_entity_assembly_naturalsource.organelle 
_em_entity_assembly_naturalsource.organism 
_em_entity_assembly_naturalsource.strain 
_em_entity_assembly_naturalsource.tissue 
? ? ? 2 1 12475 ? ? 'Hepatitis delta virus' ? ? 
? ? ? 3 2 12475 ? ? 'Hepatitis delta virus' ? ? 
# 
_em_entity_assembly_recombinant.cell                 ? 
_em_entity_assembly_recombinant.entity_assembly_id   2 
_em_entity_assembly_recombinant.id                   1 
_em_entity_assembly_recombinant.ncbi_tax_id          562 
_em_entity_assembly_recombinant.organism             'Escherichia coli' 
_em_entity_assembly_recombinant.plasmid              ? 
_em_entity_assembly_recombinant.strain               ? 
# 
loop_
_em_euler_angle_assignment.details 
_em_euler_angle_assignment.id 
_em_euler_angle_assignment.image_processing_id 
_em_euler_angle_assignment.order 
_em_euler_angle_assignment.proj_matching_angular_sampling 
_em_euler_angle_assignment.proj_matching_merit_function 
_em_euler_angle_assignment.proj_matching_num_projections 
_em_euler_angle_assignment.type 
? 1 1 INITIAL ? ? ? 'RANDOM ASSIGNMENT'  
? 2 1 FINAL   ? ? ? 'MAXIMUM LIKELIHOOD' 
# 
_em_grid_pretreatment.atmosphere          Air 
_em_grid_pretreatment.id                  1 
_em_grid_pretreatment.pressure            ? 
_em_grid_pretreatment.sample_support_id   1 
_em_grid_pretreatment.time                120 
_em_grid_pretreatment.type                'GLOW DISCHARGE' 
# 
_em_image_processing.details              ? 
_em_image_processing.id                   1 
_em_image_processing.image_recording_id   1 
# 
_em_image_recording.average_exposure_time               ? 
_em_image_recording.avg_electron_dose_per_subtomogram   ? 
_em_image_recording.avg_electron_dose_per_image         50 
_em_image_recording.details                             ? 
_em_image_recording.detector_mode                       ? 
_em_image_recording.film_or_detector_model              'GATAN K3 (6k x 4k)' 
_em_image_recording.id                                  1 
_em_image_recording.imaging_id                          1 
_em_image_recording.num_diffraction_images              ? 
_em_image_recording.num_grids_imaged                    ? 
_em_image_recording.num_real_images                     ? 
# 
loop_
_em_map.annotation_details 
_em_map.axis_order_fast 
_em_map.axis_order_medium 
_em_map.axis_order_slow 
_em_map.cell_a 
_em_map.cell_b 
_em_map.cell_c 
_em_map.cell_alpha 
_em_map.cell_beta 
_em_map.cell_gamma 
_em_map.contour_level 
_em_map.contour_level_source 
_em_map.data_type 
_em_map.dimensions_col 
_em_map.dimensions_row 
_em_map.dimensions_sec 
_em_map.endian_type 
_em_map.file 
_em_map.format 
_em_map.id 
_em_map.partition 
_em_map.entry_id 
_em_map.label 
_em_map.limit_col 
_em_map.limit_row 
_em_map.limit_sec 
_em_map.origin_col 
_em_map.origin_row 
_em_map.origin_sec 
_em_map.pixel_spacing_x 
_em_map.pixel_spacing_y 
_em_map.pixel_spacing_z 
_em_map.size_kb 
_em_map.spacing_x 
_em_map.spacing_y 
_em_map.spacing_z 
_em_map.statistics_average 
_em_map.statistics_maximum 
_em_map.statistics_minimum 
_em_map.statistics_std 
_em_map.symmetry_space_group 
_em_map.type 
'Twisted arrangement of 311-RNP, Multibody refined Body 1. Unsharpened.'  X Y Z 455.0 455.0 455.0 90.0 90.0 90.0 0.0054 author 
'Image stored as floating point number (4 bytes)' 300 300 300 big emd_71806.map.gz            CCP4 1 1 EMD-71806 
::::EMDATABANK.org::::EMD-71806:::: 299 299 299 0 0 0 1.5166667 1.5166667 1.5166667 108001024 300 300 300 -4.5227684E-6 
0.022554759 -0.014231884 5.1741826E-4 1 'primary map' 
'Twisted arrangement of 311-RNP, Multibody refined Body 1. Half map - B.' X Y Z 455.0 455.0 455.0 90.0 90.0 90.0 0.0054 author 
'Image stored as floating point number (4 bytes)' 300 300 300 big emd_71806_half_map_1.map.gz CCP4 2 1 EMD-71806 
::::EMDATABANK.org::::EMD-71806:::: 299 299 299 0 0 0 1.5166667 1.5166667 1.5166667 108001024 300 300 300 -3.2077412E-6 
0.033222657 -0.021555318 0.001362014  1 'half map'    
'Twisted arrangement of 311-RNP, Multibody refined Body 1. Half map - A.' X Y Z 455.0 455.0 455.0 90.0 90.0 90.0 0.0054 author 
'Image stored as floating point number (4 bytes)' 300 300 300 big emd_71806_half_map_2.map.gz CCP4 3 2 EMD-71806 
::::EMDATABANK.org::::EMD-71806:::: 299 299 299 0 0 0 1.5166667 1.5166667 1.5166667 108001024 300 300 300 -3.2888902E-6 0.03362768 
-0.026468378 0.0014239751 1 'half map'    
# 
_em_particle_selection.details                  'Topaz (tbepler) particle picking' 
_em_particle_selection.id                       1 
_em_particle_selection.image_processing_id      1 
_em_particle_selection.method                   ? 
_em_particle_selection.num_particles_selected   552252 
_em_particle_selection.reference_model          ? 
# 
loop_
_em_software.category 
_em_software.details 
_em_software.id 
_em_software.image_processing_id 
_em_software.fitting_id 
_em_software.imaging_id 
_em_software.name 
_em_software.version 
_em_software.reference_DOI 
'PARTICLE SELECTION'       ? 1  1 ? ? Topaz     0.2.5a ? 
'IMAGE ACQUISITION'        ? 2  ? ? 1 EPU       ?      ? 
MASKING                    ? 3  ? ? ? ?         ?      ? 
'CTF CORRECTION'           ? 4  1 ? ? cryoSPARC 4.6.0  ? 
'LAYERLINE INDEXING'       ? 5  ? ? ? ?         ?      ? 
'DIFFRACTION INDEXING'     ? 6  ? ? ? ?         ?      ? 
'MODEL FITTING'            ? 7  ? ? ? ?         ?      ? 
'MODEL REFINEMENT'         ? 8  ? ? ? ?         ?      ? 
OTHER                      ? 9  ? ? ? ?         ?      ? 
'INITIAL EULER ASSIGNMENT' ? 10 1 ? ? cryoSPARC 4.6.0  ? 
'FINAL EULER ASSIGNMENT'   ? 11 1 ? ? RELION    5.0    ? 
CLASSIFICATION             ? 12 1 ? ? ?         ?      ? 
RECONSTRUCTION             ? 13 1 ? ? RELION    5.0    ? 
# 
_em_specimen.concentration           ? 
_em_specimen.details                 ? 
_em_specimen.embedding_applied       NO 
_em_specimen.experiment_id           1 
_em_specimen.id                      1 
_em_specimen.shadowing_applied       NO 
_em_specimen.staining_applied        NO 
_em_specimen.vitrification_applied   YES 
# 
_em_start_model.details                          'Ab initio reconstruction from cryoSPARC' 
_em_start_model.emdb_id                          ? 
_em_start_model.id                               1 
_em_start_model.image_processing_id              1 
_em_start_model.insilico_model                   ? 
_em_start_model.orthogonal_tilt_angle1           ? 
_em_start_model.orthogonal_tilt_angle2           ? 
_em_start_model.orthogonal_tilt_num_images       ? 
_em_start_model.other                            ? 
_em_start_model.pdb_id                           ? 
_em_start_model.random_conical_tilt_angle        ? 
_em_start_model.random_conical_tilt_num_images   ? 
_em_start_model.type                             NONE 
# 
_em_support_film.id                  1 
_em_support_film.material            GOLD 
_em_support_film.sample_support_id   1 
_em_support_film.thickness           ? 
_em_support_film.topology            HOLEY 
# 
_pdbx_audit_support.funding_organization   'Not funded' 
_pdbx_audit_support.country                'United States' 
_pdbx_audit_support.grant_number           ? 
_pdbx_audit_support.details                ? 
_pdbx_audit_support.ordinal                1 
# 
