2025-12-17 REL RCSB RCSB RCSB 2025-07-28 2025-12-03 2025-12-03 2025-12-17 National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) 1ZIAHL006264 United States Cryo-ET subtomogram averaging of a stress fiber from a regenerating axon Bodakuntla S Taira K Yamada Y Alvarez-Brecht P Cada AK Basnet N Zhang R Martinez-Sanchez A Biertumpfel C Mizuno N cytoskeleton, F-actin, neuroregeneration, axon, STRUCTURAL PROTEIN Bodakuntla S Taira K Yamada Y Alvarez-Brecht P Cada AK Basnet N Zhang R Martinez-Sanchez A Biertumpfel C Mizuno N In situ structural mechanism of epothilone-B-induced CNS axon regeneration. Nature UK 648 477 487 2025 41224993 doi:10.1038/s41586-025-09654-z 1476-4687 0006 NATUAS EMD-71751 other EM volume
Cryo-ET reconstruction of an axon (primary mouse thalamus neuronal explant, control)
 EMD-71752 other EM volume
Cryo-ET reconstruction of a regenerating axon after axotomy showing branching microtubules (primary mouse thalamus neuronal explant)
 EMD-71753 other EM volume
Cryo-ET reconstruction of a regenerating axon after axotomy showing polymerizing microtubules (primary mouse thalamus neuronal explant)
 EMD-71754 other EM volume
Cryo-ET reconstruction of a regenerating axon 24 h after axotomy (primary mouse thalamus neuronal explant)
 EMD-71755 other EM volume
Cryo-ET reconstruction of a regenerating axon 24 h after axotomy (primary mouse thalamus neuronal explant)
 EMD-71750 other EM volume
In situ microtubule of EpoB-induced regenerating axons
 PDB 9pnd unspecified
In situ microtubule of EpoB-induced regenerating axons
 EMDB EMD-71751 other EM volume
Cryo-ET reconstruction of an axon (primary mouse thalamus neuronal explant, control)
 EMDB EMD-71752 other EM volume
Cryo-ET reconstruction of a regenerating axon after axotomy showing branching microtubules (primary mouse thalamus neuronal explant)
 EMDB EMD-71753 other EM volume
Cryo-ET reconstruction of a regenerating axon after axotomy showing polymerizing microtubules (primary mouse thalamus neuronal explant)
 EMDB EMD-71754 other EM volume
Cryo-ET reconstruction of a regenerating axon 24 h after axotomy (primary mouse thalamus neuronal explant)
 EMDB EMD-71755 other EM volume
Cryo-ET reconstruction of a regenerating axon 24 h after axotomy (primary mouse thalamus neuronal explant)
 EMDB EMD-71840 associated EM volume EMDB EMD-71750 other EM volume
In situ microtubule of EpoB-induced regenerating axons
 Thalamus primary tissue, neuron Thalamus primary tissue, neuron 0
Explant axon from thalamus primary tissue of mouse embryo
Mus musculus Brain Thalamus subtomogramAveraging cell 7.2
Gibco Neurobasal Medium
 Quantifoil R1/4 GOLD 200 SILICON DIOXIDE HOLEY GLOW DISCHARGE AIR 3.8000000000000003
coated with poly-L-lysine and laminin
 ETHANE 95 298 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 3.0 5.0 33000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum GATAN K3 BIOQUANTUM (6k x 4k) 5760 4092 61 125.0 1 1 C1 FOURIER SPACE 27.7 FSC 0.143 CUT-OFF RELION 5 1 1 1555 cylinder IMOD RELION 5 PHASE FLIPPING AND AMPLITUDE CORRECTION 1 1555.0 RELION 5 MAXIMUM LIKELIHOOD RELION 5 emd_71840.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 513.6 513.6 513.6 90.0 90.0 90.0 X Y Z -0.015425046 0.110328004 0.00077592133 0.006654625 2.675 2.675 2.675 0.02 AUTHOR postprocessed map from subtomogram averaging emd_71840_msk_1.map emd_71840_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 513.6 513.6 513.6 90.0 90.0 90.0 X Y Z -0.2764083 0.34234786 0.00031886686 0.02539278 2.675 2.675 2.675 AUTHOR half map from Refine3D emd_71840_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 513.6 513.6 513.6 90.0 90.0 90.0 X Y Z -0.27913475 0.3307439 0.00029204006 0.024657354 2.675 2.675 2.675 AUTHOR half map from Refine3D