MPER-GT12 immunogen in complex with human Fab 10E8 and mouse Fab W6-10

2025-12-10 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-08-01 2025-12-10 2025-12-10 2025-12-10 Bill & Melinda Gates Foundation INV-002916 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) UM1 AI144462 United States MPER-GT12 immunogen in complex with human Fab 10E8 and mouse Fab W6-10 Huang J Ward AB HIV, Germline targeting, MPER, 10E8, broadly neutralizing antibody, vaccine design, VIRAL PROTEIN, VIRAL PROTEIN-IMMUNE SYSTEM complex Huang J Decipher antibody responses primed with scaffolds harboring the germline-targeting HIV Env MPER epitope by cryoEM To Be Published 0353 Schiffner T Phung I Ray R Irimia A Tian M Swanson O Lee JH Lee CD Marina-Zarate E Cho SY Huang J Ozorowski G Skog PD Serra AM Rantalainen K Allen JD Baboo S Rodriguez OL Himansu S Zhou J Hurtado J Flynn CT McKenney K Havenar-Daughton C Saha S Shields K Schultze S Smith ML Liang CH Toy L Pecetta S Lin YC Willis JR Sesterhenn F Kulp DW Hu X Cottrell CA Zhou X Ruiz J Wang X Nair U Kirsch KH Cheng HL Davis J Kalyuzhniy O Liguori A Diedrich JK Ngo JT Lewis V Phelps N Tingle RD Spencer S Georgeson E Adachi Y Kubitz M Eskandarzadeh S Elsliger MA Amara RR Landais E Briney B Burton DR Carnathan DG Silvestri G Watson CT Yates JR Paulson JC Crispin M Grigoryan G Ward AB Sok D Alt FW Wilson IA Batista FD Crotty S Schief WR Vaccination induces broadly neutralizing antibody precursors to HIV gp41. Nat Immunol UK 25 1073 1082 2024 38816615 doi:10.1038/s41590-024-01833-w 1529-2916 9pv4 FULLOVERLAP EMDB EMD-71882 associated EM volume

10E8-GT12 immunogen in complex with human Fab 10E8 and mouse Fab W6-10 10E8-GT12 immunogen in complex with human Fab 10E8 and mouse Fab W6-10 0 1 2 3 4 5 synthetic construct W6-10 mouse Fab heavy chain and W6-10 mouse Fab light chain 1 1 2 Mus musculus 10E8 human Fab heavy chain and 10E8 human Fab light chain 1 4 5 Homo sapiens 10E8-GT12 immunogen 1 3 synthetic construct W6-10 mouse Fab heavy chain Mus musculus 0.013161798 1 Homo sapiens LEVO QVTLKESGPGILQPSQTLSLTCSFSGFSLSTFGMGVGWIRQPSGKGLEWLAHIWWDDDKFYNPALKSRLTISKDTSKNQV FLKIANVDTADTATYYCARIGEDYFLDVWGKGTTVTVSS W6-10 mouse Fab light chain Mus musculus 0.011657868 1 Homo sapiens LEVO DIQMTQSPASLSASVGETVTITCRASGNIHNYLAWYQQKQGKSPQLLVYNAKTLADGVPSRFSGSGSGTQYSLKINSLQP EDFGSYYCQHFWSAPYTFGGGTNLEIK 10E8-GT12 immunogen synthetic construct 0.018769171999999997 1 Homo sapiens LEVO ETGNVTQEDIIRALASPLIKDGMVDEDFAEKVIENESRYPSGLQAKGVGFAIPHVLGDYVRDNAISVGILDKPVNFSEWY QSPDPVPVRVVFMLAIRTWDDITNVMNWIKDVVLDEEFMKRLLNMSDEEIYRQIYTRISKAPNLSGINFSREYVRHLNGS GGSGLN 10E8 human Fab heavy chain Homo sapiens 0.014607246999999999 1 Homo sapiens LEVO EVQLVESGGGLVKPGGSLRLSCSASGFDFDNAWMTWVRQPPGKGLEWVGRITGPGEGWSVDYAAPVEGRFTISRLNSINF LYLEMNNLRMEDSGLYFCARTGKYYDFWSGYPPGEEYFQDWGRGTLVTVSS 10E8 human Fab light chain Homo sapiens 0.011672896 1 Homo sapiens LEVO SYELTQETGVSVALGRTVTITCRGDSLRSHYASWYQKKPGQAPILLFYGKNNRPSGVPDRFSGSASGNRASLTISGAQAE DDAEYYCSSRDKSGSRLSVFGGGTKLTVL 2-acetamido-2-deoxy-beta-D-glucopyranose 0.000221208 1 NAG singleParticle particle 2.9 7.4 50.0 Tris 150.0 sodium chloride 0.005 Lauryl Maltose Neopentyl Glycol UltrAuFoil R1.2/1.3 GOLD 300 GOLD HOLEY GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK IV TFS GLACIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 0.8 1.8 FEI FALCON IV (4k x 4k) 43.2 1 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION EMD-40825 4.0 FSC 0.143 CUT-OFF cryoSPARC 72061 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_71882.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 416 416 416 0 0 0 416 416 416 301.6 301.6 301.6 90.0 90.0 90.0 X Y Z -0.5457117 0.77172554 -0.000071126655 0.0084279515 0.725 0.725 0.725 0.1 AUTHOR ::::EMDATABANK.org::::EMD-71882:::: emd_71882_msk_1.map emd_71882_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 416 416 416 0 0 0 416 416 416 301.6 301.6 301.6 90.0 90.0 90.0 X Y Z -0.5139815 0.5908162 0.000050516977 0.03222954 0.725 0.725 0.725 AUTHOR ::::EMDATABANK.org::::EMD-71882:::: emd_71882_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 416 416 416 0 0 0 416 416 416 301.6 301.6 301.6 90.0 90.0 90.0 X Y Z -0.46755132 0.64541316 0.000039942803 0.032059316 0.725 0.725 0.725 AUTHOR ::::EMDATABANK.org::::EMD-71882::::