Cryo-EM structure of cardiac amyloid fibril from a variant apolipoprotein A-I L90P amyloidosis patient

2026-03-18 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-08-04 2026-03-18 2026-03-18 2026-03-18 American Heart Association 847236 United States National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) DP2-HL163810 United States Welch Foundation I-2121-20220331 United States Cryo-EM structure of cardiac amyloid fibril from a variant apolipoprotein A-I L90P amyloidosis patient Nguyen BA Saelices L Apolipoprotein A-I, AApoAI, L90P, Amyloidosis., PROTEIN FIBRIL Nguyen BA Saelices L Structural variability of apolipoprotein A-I amyloid fibrils across organs, mutations, and clinical presentations, revealed by cryo-EM To Be Published 0353 9pvy FULLOVERLAP EMDB EMD-71896 associated EM volume

amyloid fibrils of apoliprotein A-I amyloidosis, variant L90P amyloid fibrils of apoliprotein A-I amyloidosis, variant L90P 0 1

fibrils were extracted from cardiac tissue of a variant apolipoprotein A-I L90P amyloidosis

Homo sapiens heart heart Apolipoprotein A-I Homo sapiens HEART heart 0.028120636999999997 10 LEVO DEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETE GLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALR THLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKL NTQ P02647 helical filament 7.0

H2O containing 5 mM EDTA

Quantifoil R1.2/1.3 COPPER 300 CARBON HOLEY GLOW DISCHARGE 30 AIR ETHANE 100 298 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.8 2.6 FEI FALCON IV (4k x 4k) 1 12645 5.0 40.0 1 1 4.85 -0.68 C1 2.15 FSC 0.143 CUT-OFF RELION 4.0 118342 CTFFIND

RELION 4 built in CTFFIND

NONE initial model was generated using relion_helix_inimodel2d. NOT APPLICABLE RELION 4.0 emd_71896.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 238.592 238.592 238.592 90.0 90.0 90.0 X Y Z -0.13357697 0.24080488 0.0000663148 0.008159757 0.932 0.932 0.932 0.0322 AUTHOR ::::EMDATABANK.org::::EMD-71896:::: post-processed map that was used to build protein model. Pixel was calibrated. OTHER

This is a novel structure. Model was built using the protein primary sequence by COOT

REAL emd_71896_msk_1.map emd_71896_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 244.224 244.224 244.224 90.0 90.0 90.0 X Y Z -0.04510304 0.07626108 0.00009446226 0.0048905555 0.954 0.954 0.954 AUTHOR ::::EMDATABANK.org::::EMD-71896:::: combined map from two half-maps emd_71896_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 244.224 244.224 244.224 90.0 90.0 90.0 X Y Z -0.04816378 0.07859802 0.00009387854 0.0050456077 0.954 0.954 0.954 AUTHOR ::::EMDATABANK.org::::EMD-71896:::: half-map 02 emd_71896_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 244.224 244.224 244.224 90.0 90.0 90.0 X Y Z -0.045643814 0.080187954 0.00009511783 0.005041479 0.954 0.954 0.954 AUTHOR ::::EMDATABANK.org::::EMD-71896:::: half-map 01