2026-01-14 REL RCSB RCSB RCSB 2025-08-07 2025-11-12 2025-11-12 2026-01-14 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM144965 United States National Institutes of Health/National Center for Complementary and Integrative Health (NIH/NCCIH) AT012075 United States Engaged-state loperamide-mu opioid receptor-Gi GDP complex (constant GDP) - Locally refined map Gati C Khan S Han GW G protein coupled receptor, Mu Opioid receptor, Loperamide, MEMBRANE PROTEIN, GDP Khan S Tyson AS Ranjbar M Zhang Z Singh J Han GW Gati C Structural snapshots capture nucleotide release at the mu-opioid receptor. Nature UK 648 755 763 2025 41193810 doi:10.1038/s41586-025-09677-6 1476-4687 0006 NATUAS EMD-72024 consensus EM volume EMDB EMD-72024 consensus EM volume EMDB EMD-72025 associated EM volume Loperamide-mu opioid receptor in complex in complex with Gai-GDP, Gb1 and Gg2 Loperamide-mu opioid receptor in complex in complex with Gai-GDP, Gb1 and Gg2 0 1 2 3 4 Homo sapiens 130 singleParticle particle 7.5 40.0 HEPES HEPES 100.0 NaCl sodium chloride 0.00075 LMNG detergent 0.00025 GDN detergent UltrAuFoil R1.2/1.3 GOLD GOLD HOLEY ARRAY ETHANE 95 298 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.5 2.5 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 1 51.0 1 cryoSPARC v4.3.1 PHASE FLIPPING AND AMPLITUDE CORRECTION Ab initio 4.44 FSC 0.143 CUT-OFF cryoSPARC v4.3.1 20316 ANGULAR RECONSTITUTION cryoSPARC v4.3.1 ANGULAR RECONSTITUTION cryoSPARC v4.3.1 2 cryoSPARC v4.3.1 emd_72025.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 331.264 331.264 331.264 90.0 90.0 90.0 X Y Z -0.26080683 0.5292527 -0.0002823209 0.015070601 1.294 1.294 1.294 0.131 AUTHOR Engaged-state loperamide-mu opioid receptor-Gi GDP complex (constant GDP) - Locally refined map emd_72025_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 331.264 331.264 331.264 90.0 90.0 90.0 X Y Z -0.16461405 0.43721598 -0.0002823209 0.01284589 1.294 1.294 1.294 AUTHOR Additional Map emd_72025_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 331.264 331.264 331.264 90.0 90.0 90.0 X Y Z -0.48604107 0.6121194 0.00023905505 0.0561368 1.294 1.294 1.294 AUTHOR Half Map A emd_72025_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 331.264 331.264 331.264 90.0 90.0 90.0 X Y Z -0.40339875 0.6500274 0.00023441309 0.055437308 1.294 1.294 1.294 AUTHOR Half Map B