2025-09-17 REL RCSB INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-08-08 2025-09-17 2025-09-17 2025-09-17 National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) AI158463 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) AI171292 United States SARS-CoV-2 nsp7, nsp8 and nsp12 bound to a primer-template pair with incorporated ara-UMP Xiao Z Kirchdeorfer RN RNA-dependent RNA polymerase (RdRp), Viral RNA synthesis, arabinose UTP, nsp7, nsp8, nsp12, VIRAL PROTEIN Xiao Z Das A Jain A Anderson TK Cameron CE Arnold JJ Dulin D Kirchdoerfer RN The 2'-endo conformation of arabinose-CTP and arabinose-UTP inhibit viral polymerases by inducing long pauses. Biorxiv US 2025 40909592 doi:10.1101/2025.08.26.672356 2692-8205 9pyw FULLOVERLAP PDB 9BLF unspecified PDB 9PZ0 unspecified
SARS-CoV-2 nsp7, nsp8 and nsp12 bound to a primer-template pair
 PDB 9PYZ unspecified
SARS-CoV-2 nsp7, nsp8 and nsp12 bound to a primer-template pair with incorporated ara-UMP and UTP
 EMDB EMD-72038 associated EM volume
SARS-CoV-2 nsp7, nsp8 and nsp12 bound to a primer-template pair with incorporated ara-UMP
 SARS-CoV-2 nsp7, nsp8 and nsp12 bound to a primer-template pair with incorporated ara-UMP SARS-CoV-2 nsp7, nsp8 and nsp12 bound to a primer-template pair with incorporated ara-UMP 0 1 2 3 4 5 Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase 1 1 Severe acute respiratory syndrome coronavirus 2 Replication factors of RNA-dependent RNA polymerase 1 2 3 Severe acute respiratory syndrome coronavirus 2 RNA 1 4 5 synthetic construct RNA-directed RNA polymerase nsp12 Severe acute respiratory syndrome coronavirus 2 0.110424898 1 Spodoptera frugiperda LEVO MGSADAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFS NYQHEETIYNLLKDCPAVAKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFN KKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYY SLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDRCILHCANFNVLFS TVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSV AALTNNVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDK YFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVA GVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVL ARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADK YVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAK CWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYA DVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQENLYFQGSWSHPQFEKGGSGSSWSHP QFEK P0DTD1 2.7.7.48 Non-structural protein 8 Severe acute respiratory syndrome coronavirus 2 0.021960099999999996 2 Escherichia coli BL21(DE3) LEVO GAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDK RAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQV VDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQ P0DTD1 Non-structural protein 7 Severe acute respiratory syndrome coronavirus 2 0.009305854999999998 1 Escherichia coli BL21(DE3) LEVO GSKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNR ATLQ P0DTD1 Primer RNA Severe acute respiratory syndrome coronavirus 2 0.009487689 1 CAUUCUCCUAAGAAGCUAUUAAAAUCACA(UAR) Template RNA Severe acute respiratory syndrome coronavirus 2 0.012088101 1 UUUUCCCAAUGUGAUUUUAAUAGCUUCUUAGGAGAAUG ZINC ION 6.5409e-05 2 ZN MAGNESIUM ION 2.4305e-05 1 MG singleParticle particle 8 8.0 UltrAuFoil R1.2/1.3 GOLD 300 GOLD HOLEY GLOW DISCHARGE AIR ETHANE 100 277 FEI VITROBOT MARK IV FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 70.0 1.0 2.5 GIF Bioquantum 20 EPU 3.6.0.6389 GATAN K3 BIOQUANTUM (6k x 4k) 1 3479 4.45 55.0 1 NONE 9BLF C1 3.1 FSC 0.143 CUT-OFF cryoSPARC v4.4.0 621798 RANDOM ASSIGNMENT MAXIMUM LIKELIHOOD emd_72038.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 272.384 272.384 272.384 90.0 90.0 90.0 X Y Z -2.5964906 3.6746454 0.0015831514 0.084907286 1.064 1.064 1.064 0.3 AUTHOR 9BLF PDB experimental model REAL emd_72038_msk_1.map emd_72038_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 272.384 272.384 272.384 90.0 90.0 90.0 X Y Z -0.24802746 0.76004237 0.0013352889 0.025871657 1.064 1.064 1.064 AUTHOR emd_72038_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 272.384 272.384 272.384 90.0 90.0 90.0 X Y Z -0.27052456 0.7544132 0.0013326188 0.025888717 1.064 1.064 1.064 AUTHOR