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    <admin>
        <current_status>
            <date>2025-12-10</date>
            <code>REL</code>
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        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
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        <key_dates>
            <deposition>2025-08-08</deposition>
            <header_release>2025-12-03</header_release>
            <map_release>2025-12-03</map_release>
            <update>2025-12-10</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Howard Hughes Medical Institute (HHMI)</funding_body>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Cryo ET of Native Hippocampal Glutamatergic Synapses without AuNP label 3</title>
        <authors_list>
            <author ORCID="0000-0002-1897-4319">Kim H</author>
            <author ORCID="0000-0003-2334-3957">Spangler CJ</author>
            <author ORCID="0000-0003-4437-8278">Matsui A</author>
            <author ORCID="0000-0002-9911-706X">Elferich J</author>
            <author ORCID="0000-0002-8549-2360">Gouaux E</author>
        </authors_list>
        <keywords>Glutamatergic synapse, NEUROPEPTIDE</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Kim H</author>
                    <author order="2">Spangler CJ</author>
                    <author order="3">Matsui A</author>
                    <author order="4">Elferich J</author>
                    <author ORCID="0000-0001-5866-9952" order="5">Kim J</author>
                    <author order="6">Roseborough A</author>
                    <author ORCID="0000-0002-1787-0518" order="7">Nyman M</author>
                    <author order="8">Lahtinen TM</author>
                    <author ORCID="0000-0002-8549-2360" order="9">Gouaux E</author>
                    <title>Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography.</title>
                    <journal_abbreviation>Proc.Natl.Acad.Sci.USA</journal_abbreviation>
                    <country>US</country>
                    <volume>122</volume>
                    <first_page>e2524034122</first_page>
                    <last_page>e2524034122</last_page>
                    <year>2025</year>
                    <external_references type="PUBMED">41284882</external_references>
                    <external_references type="DOI">doi:10.1073/pnas.2524034122</external_references>
                    <external_references type="ISSN">1091-6490</external_references>
                    <external_references type="CSD">0040</external_references>
                    <external_references type="ASTM">PNASA6</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-72058</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Glutamatergic synapse from mouse hippocampus tissue</name>
        <supramolecule_list>
            <tissue_supramolecule supramolecule_id="1">
                <name>Glutamatergic synapse from mouse hippocampus tissue</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                    <strain>C57BL/6</strain>
                    <organ>Brain</organ>
                    <tissue>Hippocampus</tissue>
                </natural_source>
            </tissue_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
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            <method>tomography</method>
            <aggregation_state>tissue</aggregation_state>
            <specimen_preparation_list>
                <tomography_preparation preparation_id="1">
                    <buffer>
                        <ph>7.4</ph>
                    </buffer>
                    <grid>
                        <model>EMS Lacey Carbon</model>
                        <material>GOLD</material>
                        <mesh>200</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>CONTINUOUS</film_topology>
                            <film_thickness>2.0</film_thickness>
                        </support_film>
                    </grid>
                    <vitrification>
                        <cryogen_name>NITROGEN</cryogen_name>
                    </vitrification>
                    <fiducial_markers_list>
                        <fiducial_marker>
                            <manufacturer>Gikd nanoparticle</manufacturer>
                            <diameter units="nanometer">10</diameter>
                        </fiducial_marker>
                    </fiducial_markers_list>
                    <high_pressure_freezing>
                        <instrument>OTHER</instrument>
                        <details>The value given for _em_high_pressure_freezing.instrument is Leica ICE. This is not in a list of allowed values {'LEICA EM PACT2', 'LEICA EM PACT', 'BAL-TEC HPM 010', 'EMS-002 RAPID IMMERSION FREEZER', 'OTHER', 'LEICA EM HPM100'} so OTHER is written into the XML file.</details>
                    </high_pressure_freezing>
                    <cryo_protectant>20 % dextran /5 % sucrose</cryo_protectant>
                    <sectioning>
                        <focused_ion_beam>
                            <instrument>OTHER</instrument>
                            <ion>OTHER</ion>
                            <voltage>30</voltage>
                            <current>0.03</current>
                            <duration>120</duration>
                            <temperature units="K">83</temperature>
                            <initial_thickness>40000</initial_thickness>
                            <final_thickness>200</final_thickness>
                            <details>The value given for _em_focused_ion_beam.instrument is TFS Aquilos 2. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.</details>
                        </focused_ion_beam>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_cs units="mm">0.01</nominal_cs>
                    <nominal_defocus_min units="µm">4.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">4.0</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 BIOCONTINUUM (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">50.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </tomography_microscopy>
            </microscopy_list>
            <tomography_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <software_list>
                        <software>
                            <name>IMOD</name>
                        </software>
                    </software_list>
                    <number_images_used>33</number_images_used>
                </final_reconstruction>
                <ctf_correction>
                    <software_list>
                        <software>
                            <name>MotionCorr2</name>
                        </software>
                    </software_list>
                    <type>NONE</type>
                </ctf_correction>
            </tomography_processing>
        </structure_determination>
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        <file>emd_72058.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
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        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
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            <col>778</col>
            <row>778</row>
            <sec>375</sec>
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            <row>0</row>
            <sec>0</sec>
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        <spacing>
            <x>778</x>
            <y>778</y>
            <z>375</z>
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        <cell>
            <a units="Å">7780.0</a>
            <b units="Å">7780.0</b>
            <c units="Å">3750.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
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        <statistics>
            <minimum>-1.6058419</minimum>
            <maximum>0.86986285</maximum>
            <average>0.038046073</average>
            <std>0.049629718</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">10.0</x>
            <y units="Å">10.0</y>
            <z units="Å">10.0</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-72058::::</label>
    </map>
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