2026-01-21 REL RCSB RCSB RCSB 2025-08-14 2025-12-10 2025-12-10 2026-01-21 National Institutes of Health/National Cancer Institute (NIH/NCI) U01CA295050 United States Negative Stain EM map of KSHV glycoprotein gHgL in complex with MLKH5, MLKH10 and MLKH6 FABs Lang K Aldridge NT Pancera M Viral Protein, Complex, Antibody, KSHV, IMMUNE SYSTEM Wan YH Pernikoff S Aldridge NT Lang K Dudley HM Scharffenberger SC Kher G Phipps W Pancera M Boonyaratanakornkit J McGuire AT Monoclonal neutralizing antibodies elicited by infection with Kaposi sarcoma-associated herpesvirus reveal critical sites of vulnerability on gH/gL. Plos Pathog. US 22 e1013772 e1013772 2026 41499715 doi:10.1371/journal.ppat.1013772 1553-7374 EMDB EMD-72133 associated EM volume KSHV glycoprotein heterodimer gHgL 1:1 MLKH5, MLKH10 and MLKH6 FABs KSHV glycoprotein heterodimer gHgL 1:1 MLKH5, MLKH10 and MLKH6 FABs 0 1 2 3 4 5 6 7 8 0.242 Glycoprotein gH Human gammaherpesvirus 8 GK18 Homo sapiens LEVO MDAMKRGLCCVLLLCGAVFVSPSASATGALPTTATTITRSATQLINGRTNLSIELEFNGTSFFLNWQNLLNVITEPALTELWTSAEVAEDLRVTLKKRQSLFFPNKTVVISGDGHRYTCEVPTSSQTYNITKGFNYSALPGHLGGFGINARLVLGDIFASKWSLFARDTPEYRVFYPMNVMAVKFSISIGNNESGVALYGVVSEDFVVVTLHNRSKEANETASHLLFGLPDSLPSLKGHATYDELTFARNAKYALVAILPKDSYQTLLTENYTRIFLNMTESTPLEFTRTIQTRIVSIEARRACAAQEAAPDIFLVLFQMLVAHFLVARGIAEHRFVEVDCVCRQYAELYFLRRISRLCMPTFTTVGYNHTTLGAVAATQIARVSATKLASLPRSSQETVLAMVQLGARDGAVPSSILEGIAMVVEHMYTAYTYVYTLGDTERKLMLDIHTVLTDSCPPKDSGVSEKLLRTYLMFTSMCTNIELGEMIARFSKPDSLNIYRAFSPCFLGLRYDLHPAKLRAEAPQSSALTRTAVARGTSGFAELLHALHLDSLNLIPAINCSKITADKIIATVPLPHVTYIISSEALSNAVVYEVSEIFLKSAMFISAIKPDCSGFNFSQIDRHIPIVYNISTPRRGCPLCDSVIMSYDESDGLQSLMYVTNERVQTNLFLDKSPFFDNNNLHIHYLWLRDNGTVVEIRGMYGSGSGHHHHHH F5HAK9 Glycoprotein gL Human gammaherpesvirus 8 GK18 Homo sapiens LEVO MDAMKRGLCCVLLLCGAVFVSPSASDGIQYVALPCCAIQASAASTLPLFFAVHSIHFADPNHCNGVCIAKLRSKTGDITVETCVNGFNLRSFLVAVVRRLGSWASQENLRLLWYLQRSLTAYTVGFNATTADSSIHNVNIIIISVGKAMNRTGSVSGSQTRAKSSSRRAHAGQKGK F5HDB7 MLKH5 heavy chain Mus musculus Homo sapiens LEVO MGWSCIILFLVATATGVHSQVQLVESGGGVVQPGRSLRLSCAASGFIFSSYGMHWVRQAPGKGLEWIAIIWYDGSHKYYADSVKGRFTI SRDNSKNTLYLQMNSLRAEDTAVYYCARDPGYCSRYTCYGGWFDPWGQGTLVTVSSASTKGPSVFPLAPS SKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICN VNHKPHHHHHH MLKH5 light chain Mus musculus Homo sapiens LEVO MGWSCIILFLVATATGVHSDIQMTQSPSSLSASVGDRVTITCRASQNINNYLNWYQQKPGKAPKVLIYAASSLQSGVPSRFSGSGSGTD FTLTINSLQPEDFATYYCQRSSNSPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFY PREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFN RGEC MLKH6 heavy chain Mus musculus Homo sapiens LEVO MGWSCIILFLVATATGVHSQVQLQESGPGSVRPSQTLSLTCTVSGGSITSGTYYWTWIRQPAGKGLEWIGYISSTGNTNYNPSLKSRVT MALDTSKNQFSLKLSSVTAADTAVYHCARGRRTTGIAAAVCDSWGQGTLATVSSASTKGPSVFPLAPSSK STSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN HKPHHHHHH MLKH6 light chain Mus musculus Homo sapiens LEVO MGWSCIILFLVATATGVHSEIVMTQSPATLSVSPGERATLSCRASQSLTTNLAWYQQKPGQAPRLLIYAASTRATGIPARFSGMSGSGT EFTLTISNMQSEDFAIYYCQQYNNWPPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNN FYPREAKVQWKVDNALQSGNSQESVTEEDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHGGLSSPVTKS FNRGE MLKH10 heavy chain Mus musculus Homo sapiens LEVO MGWSCIILFLVATATGVHSEVQLLESGGGVVRPGGSLRLSCAASGFTFDDYGMSWVRQSPGKGLEWVSGINWNGGSLSYADSIQGRFTI SRDNAENSLYLQMHSLRAEDTALYYCARVQGSGSYNNFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTS GGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPHHHHHH MLKH10 light chain Mus musculus Homo sapiens LEVO MGWSCIILFLVATATGVHSEVDIVMTQSPDSLSVSLGERATINCKSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASTRGSGVPDRFSG SGSGTDFTLTISSLQAEDVAVYYCQQYYNSPRFGGWTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCL LNNFYPREAKVQWKVDNALQSGNSQESVTEHDSKDSTYSLSSTLTLTKADYEKHKVYACEVTHRATARPS QRASTRESV singleParticle particle 7.5
1x TBS
 NEGATIVE Uranyl Formate
2% Uranyl Formate
 TFS TALOS L120C FLOOD BEAM BRIGHT FIELD LAB6 120 70.0 2.0 0.3 2.0 96000.0 NITROGEN FEI CETA (4k x 4k) 40.0 1 NONE 24.15 FSC 0.143 CUT-OFF cryoSPARC 4.7.0 1881 MAXIMUM LIKELIHOOD cryoSPARC 4.7.0 MAXIMUM LIKELIHOOD cryoSPARC 4.7.0 emd_72133.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 120 120 120 0 0 0 120 120 120 549.6 549.6 549.6 90.0 90.0 90.0 X Y Z -6.1814847 20.134663 0.004402709 0.67094815 4.58 4.58 4.58 3.05 AUTHOR emd_72133_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 348 348 348 0 0 0 348 348 348 549.84 549.84 549.84 90.0 90.0 90.0 X Y Z -4.5989313 5.871865 0.0009092113 0.467449 1.58 1.58 1.58 AUTHOR emd_72133_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 348 348 348 0 0 0 348 348 348 549.84 549.84 549.84 90.0 90.0 90.0 X Y Z -4.8597646 6.013424 0.0009960489 0.460024 1.58 1.58 1.58 AUTHOR