2025-12-10 REL RCSB RCSB RCSB 2025-09-08 2025-12-10 2025-12-10 2025-12-10 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01 GM141109 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01 GM143183 United States National Science Foundation (NSF, United States) MCB-2434879 United States Leishmania 96 nm half 1 protofilament refinement position 7_2 Doran MH Brown A flagella, parasite, axoneme, doublet microtubule, STRUCTURAL PROTEIN Fochler S Doran MH Beneke T Smith J Fort C Walker BJ Brown A Gluenz E Wheeler RJ Axonemal dynein contributions to flagellar beat types and waveforms. Biorxiv US 2025 41279373 doi:10.1101/2025.11.06.687033 2692-8205 EMDB EMD-72582 associated EM volume Leishmania 96 nm half 1 protofilament refinement position 7_2 Leishmania 96 nm half 1 protofilament refinement position 7_2 0 Leishmania tarentolae P10 Flagella singleParticle filament 6.5 7.2 Quantifoil R2/1 COPPER 300 CARBON HOLEY ETHANE 100 278 FEI VITROBOT MARK IV
This sample consisted of freshly splayed Leishmania tarentolae axonemes.
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.5 2.0 NITROGEN GATAN K3 (6k x 4k) 4 37665 62.6 1 CTFFIND PHASE FLIPPING AND AMPLITUDE CORRECTION 3.5 FSC 0.143 CUT-OFF RELION 251436 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION emd_72582.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 680.96 680.96 680.96 90.0 90.0 90.0 X Y Z -0.0047405423 2.6264613 0.001415108 0.028482353 1.33 1.33 1.33 0.124 AUTHOR DeepEMhancer sharpened map Other integrative model
The initial model consisted of rigid body fit AlphaFold models and models build with ModelAngelo.
 emd_72582_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 680.96 680.96 680.96 90.0 90.0 90.0 X Y Z -0.031023724 0.052181173 -0.0000055609535 0.0010769725 1.33 1.33 1.33 AUTHOR Relion sharpened map emd_72582_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 680.96 680.96 680.96 90.0 90.0 90.0 X Y Z -0.031024063 0.046632025 0.0016902406 0.0033157305 1.33 1.33 1.33 AUTHOR Half map 2 emd_72582_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 680.96 680.96 680.96 90.0 90.0 90.0 X Y Z -0.03161006 0.044965845 0.0017194353 0.0033517696 1.33 1.33 1.33 AUTHOR Half map 1