Cryo-EM structure of intraconoidal microtubule 2 (ICMT2) from Toxoplasma gondii (8-nm repeat)

2026-02-04 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation_author em_admin _em_admin.last_update EXPERIMENTAL_SUMMARY citation_author em_admin _em_admin.last_update DATABASE_REFERENCES citation em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update DATABASE_REFERENCES citation em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _em_admin.last_update RCSB RCSB 2025-09-15 2025-12-03 2025-12-03 2026-02-04 National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI179885 United States Cryo-EM structure of intraconoidal microtubule 2 (ICMT2) from Toxoplasma gondii (8-nm repeat) Zeng J Zhang R Tubulin, Microtubule, Microtubule Inner Protein, Microtubule-associated Protein, Toxoplasma gondii, conoid, conoid fiber, intraconoidal microtubule, ICMT, STRUCTURAL PROTEIN Zeng J Fu Y Qian P Huang W Niu Q Beatty WL Brown A Sibley LD Zhang R Atomic models of the Toxoplasma cell invasion machinery. Nat.Struct.Mol.Biol. US 33 157 170 2026 41366525 doi:10.1038/s41594-025-01728-w 1545-9985 9ya1 FULLOVERLAP EMDB EMD-72717 associated EM volume

conoid fiber from Toxoplasma gondii (24-nm repeat) conoid fiber from Toxoplasma gondii (24-nm repeat) 0 1 2 3 4 5 6 7 8 Toxoplasma gondii ICMAP4, TGME49_225340 Toxoplasma gondii 0.230114188 2 LEVO MLSRMPEPACGSSYLGPPSLNLPSFPSGSCGATANSALRRLPEPCREQLERDLAVFARGSTSGVPPLPNASNKPVCPAAG QARMDRVKHRDSERSLSSQKFEGNEWGKKKRVFETETPDLTGHLRPVPGDSLRATGQLADNAAYFAPYSLTSRGKGPDTR DSDAENSGLEELADIGCGWEETESDAQLGGLSDAGSVSTAAPTDTPAALPGLEGLRRQTDDLNEWLDHSIRTLLHAARYQ LSETADSDHPSEADAVEAARLEYQEALEKIQDARLKDRAKSDVHIAKLQSQLQKAQAECQNLQSTITEKDALLRQLQAHV KALRSGRASKSVSEAQPTEVDALFANLLNPDEGEAFAEDNPVANLTQASQRLAQNVTGGPKERKNAGAPTPADVLKTEFE LKLQSLRSIKGSNAPALQEWLRVFVSSWKTLQTRLKSVSIPEKPTEKVLQLAKEKIEKERALTFKALVQVQQECLRVMKQ VFETPWRAEELARENKEQTAKISLLSSDVEKTREHTAVLAAELKRRGRLIANLEEAVHMLSENLSLCQEDLSTRFEQVLE SREKWKEAETELQRTQATLKVTKKEAAKLETHLEEVLGQYRASQRHARDLQVQLEDRTSCLHGVQDDLNRFEKANAELAD DLHATRIELTKTAIELHAEQLAASQERSSEISEWKERQRRIEENILEALKKSEPKAASESSRGFPIRIHSPRTRPTGAFS RLRSSRRSPPKTRRGLTRLVSAVARTGEVERKLQENAEIVAGAKQATAGARDKGSLENLDETWKSIMQTVKKVERQVQSV YSSRRSHTVFARELRGLDRLLEGLEPQFVRAHKAFESLKLQASGEAAARFHQASELRAKDVAQAPLEANIRSTRPKGDQA LRWADNLSGKLLGKKNQGRRSRFGKKMETRGLAVTRRGSRSSADFKREHSDSAWANVNDSDDERERNVPLSPSASATLKE ESSRSEGTDSAEREFASSLSKSRSFVSEDRGRDAFPGFQGLQKKLRRFSSTGSTPLSDDSYSFSGVNRRHRRNRSVPPGR SPSQRNGDHAAAPLVHEVKRLEGELKRAQEIIKEIEETLLVPQDYFRKMREVTEENIRLNLLVCSLRADIEHCKVDRQDL ESFHQVRVDYAQFQAETEKRIRDLEKQLKVEAAAARRSSLPDAAVRDAAGVARKKLENLRVEHQLEIRTLRDMNALEVER MRQNYEREISSLQQMIQTVKDNTESEYKKKLQLLQLQKETTMEKFRSELETQLRETKREYERELQEKQKDKVEALYKAEI AQQAALTEASRDFNKRLKEVEKHYEDRLLEHQVHVVKERDGLIARFKTKIENLTSQLEEQKEARWKAREAQDQAEVERKR HLAELERQLRVETLEKDELREALERQTSRAESLMEELGTVRREGGSPNIGRLADRGCQVEPTVRSRGTQVEDTVMEYIRE CTEISEEAMQGDETGRVRSRVRDMLDERNGNLRGRCLLRHEPTFLSSPPAEGLCSRKESGEKSRKASVLEVEDDASIDGA VQAVENGVLKTASKAGTTTPSEGKGLKMLGTRTSIGCGVEKEDQAGKKEADLFSKVADVAFRAKAGSVGPFQRRGSEAEQ KDQQADTSGQSRGERGAEISERNTTSDGLGSKDPSGRDKSPAVTGMKHYRDIKETPSIDKKLHADEDEGRQARDLSRSSS RDGPRSSSRDFDSFAVVRSKALEVLRRQRADPSDWEGCENVEKDHFGSRERHSNGEEFKTQGNVGRGSLRQEPFTDGVYH DRQQRFSEKEPAKPMFTSLADPSVRRHFKEEEERRKFQEKAEEEILRLLKRAAECSEEDLKREERSEKATEKGSRLFSGE EVRFFPASLDSELERLKERLRPGNSVLKAVRDTGAFSKFGSDDNSARSDRSRHNERRSSLSGAGTGVDDTFGNSRADNLR VEPASRRHTGEYARGTSFHGNRRPETVERQEKLVAGRSLRTSLIETLSATSHQIHVERSAVKCGLDHMLQRQEHSGWFPG RTENSKESGGTQATISEQMRALESLEMLSRRVRTGRRDAKQ A0A086KM91 Microtubule associated protein SPM1 Toxoplasma gondii 0.03884575 11 LEVO MSGGNSNTPKKLPSEEGSDYGYPQKPQKYLPKSEQAEPDYSACCKGNDAYKGASHGTVQFSHPEEAQKYAGAAAGAETIQ RGRERVAADRQPRAAGDVPARRLHLSDVDEAHRGQSPSRHPGYCVEELCTCGMHKCIPSRAPVPFTGSTQYRQEFVPKPL PPPTQVSQVTLPPSLPFEAESSYRTEFVAKPLPPPAKFSEVKLPPTLPFHGESAYRTDYVPKPLPEVAKPVEVKLPPTLP FNAQSCYRSEYVAKPLPPPVQTVEVKLPPSLPFEGSTHYRDEFQVKPLPPATKVTEVKLPPSLPFDATSMYRSDYVAKSN PICPVSKLPQYPAATYPQNHVFWDPDTKQWY A0A7J6K285 Tubulin alpha chain Toxoplasma gondii 0.050166644999999996 26 LEVO MREVISIHVGQAGIQIGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRCVFLDLEPTVVDEVRT GTYRHLFHPEQLISGKEDAANNFARGHYTIGKEIVDLSLDRIRKLADNCTGLQGFLMFNAVGGGTGSGLGCLLLERLSVD YGKKSKLNFCSWPSPQVSTAVVEPYNSVLSTHSLLEHTDVAVMLDNEAIYDICRRNLDIERPTYTNLNRLIAQVISSLTA SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPIISAEKAYHEQLSVAEITNSAFEPASMMAKCDPRHGKYMACCLMYR GDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVMRAVCMISNSTAIAEVFSRMDHKFDLMYA KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGIETAEGEGEEEGYGDEY P10873 Tubulin beta chain Toxoplasma gondii 0.050119120999999996 26 LEVO MREIVHVQGGQCGNQIGAKFWEVISDEHGIDPTGTYCGDSDLQLERINVFYNEATGGRFVPRAILMDLEPGTMDSVRAGP FGQLFRPDNFVFGQTGAGNNWAKGHYTEGAELIDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGSGMGTLLISKVREEYP DRIMETFSVFPSPKVSDTVVEPYNATLSVHQLVENADEVQVIDNEALYDICFRTLKLTTPTYGDLNHLVSAAMSGVTCCL RFPGQLNSDLRKLAVNLIPFPRLHFFLIGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMCASDPRHGRYLTASAMFRGR MSTKEVDEQMLNVQNKNSSYFVEWIPNNMKSSVCDIPPKGLKMSVTFVGNSTAIQEMFKRVSDQFTAMFRRKAFLHWYTG EGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFDEEEGEMGAEEGA I7BFC9 TLAP3 (apical cap protein AC5), TGME49_235380 Toxoplasma gondii 0.065286098 3 LEVO MPRPGVFELNYGEESRHSPVPKDPSTVTYGDASVGVVHHPLPAAGMYRQPCAFPHGSGFSKQSNETAFPDYPSTEHVAQR YVPPMDDKRQWAGHTSYYAPATMEQRGYQDARYFGAELMPKSQGGENPQNHPIMPSEAAEYGPRKPDCGRLIAAPHEGQV NNLSQVDGRICGTLPVIDNQSFQVEPCFRAPTIATGIPRLQTMKGRDAFAQDATRQRALQGHRTADLLKTPFDYDLFHRT RLPPSAGASIQAAGKEIDWSEKKLFRKAVVSTVFASDQVAERLRQDLPSPNTRDGQRTNADHGNRRNWSENIESLLRQAT PVESSAENDPIFTAKVEERSFDSSLLYHGCRHLKKRSAFGHTPDAVAQLLRSSAEAGKGERPLVPSRPSRPSQHDSTQLK RVLSYHPDPEADSKGCFEQRARLRRSQLVTAKNSCRWGVNVFGYDLSPPRRNRLYALRDHLDSKLVPNQSTDDATHIKPR HTNVLSTSLHMSKLVPVTDLSPRPSFRYHADTGSLDATLLPVDAVPQERIGRRLISPPESSLQSNFVPSHEEVGRHKRFL VNSRDSLQGNMIPIVKECTRRRK A0A151H4L4 TLAP4 (thioredoxin-like associated protein), TGME49_201760 Toxoplasma gondii 0.03736023 4 LEVO MERSGHLCKMEPWKQKVPQCLSLHGSNSLRGGKRTYNCNVLMDNWRNDRLDAHNDQKLTSWRIPHPLYTELPQGSKYETH YRQSYDYEDWARRPVRLSKYGLLGHDDSGAESWTTETRSEYEPPRLRRDQLKAAGQWPYGEDIYPRHVHGNEEFMCPHPM AIHVSRTHREDYIRSLTPRPAYERPPAGVPSSSGRLPGVPPYANDPISHHGGSQEQRHFCRMPCRNPSGSSSVQGTTGVD ARASPAGSPVFAGQVPAQVKQEGGECGQGYAGIAATEDGLVDHGNSQTDRSAGQHCMLNASGGSERAHAAANTSTCPQRK ESDPQPTVSWAAVPCQ A0A7J6K913 TRXL1, TGME49_232410 Toxoplasma gondii 0.024939331999999998 22 LEVO MSQPVFASPLNVEKRRLNEERALMQAQKAGGEGVNIQLPPNYGDMDLILFPEGSLKNSNNTVIPQSHLKGKSVALYFADG ADPKCASLLPFLLNYYRTMNEGGANQKIEIIFVSLDRDREAFESHRAHMPWLSIDLENPLTEILKRHFRVMKEYEVPTYG YGSRTGVPSVIVIGSDGREAQFLPICSGLEEGDRALLRWDWRNTKFASDQFHVRPTLLEQ Q8MPF4 TRXL2, TGME49_225790 Toxoplasma gondii 0.021687934 2 LEVO MLAADCFFGPDVVKRTQQGNYVPVRPDHFAGVSVALFFAKAGHSKCAQIVPVVRQFYKTTNFSGEKAVIEIIYVSLDKDE QDFERVRALMPWCSVEYKSCLRKKLIERYRVPNGELAFGTVRIPSTAIPLLIVIGPNGEEAGRMNFQQSDEFVLQRWDYR FNKWPGSAQRLRTLNDATDPWKKRLPQNV A0A7J6K232 GUANOSINE-5'-TRIPHOSPHATE 0.0005231799999999999 26 GTP MAGNESIUM ION 2.4305e-05 26 MG GUANOSINE-5'-DIPHOSPHATE 0.000443201 26 GDP singleParticle filament 7.5 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 5.0 25.0 GATAN K3 BIOQUANTUM (6k x 4k) 50.0 1 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION 3.52 FSC 0.143 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