2025-12-31 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-10-10 2025-12-31 2025-12-31 2025-12-31 National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) R01HL145473 United States Constituent EM map: Focused refinement on JSol+CSol of mouse RyR1 (including auxiliary transmembrane helix TMx; EGTA-only dataset) Weninger G Marks AR Calcium, Ion Channel, Membrane Protein Weninger G Dridi H Reiken S Yuan Q Zhao N Groom L Leigh J Liu Y Tchagou C Kang J Chang A Luna-Figueroa E Miotto MC Wronska A Dirksen RT Marks AR Structural basis for simvastatin-induced skeletal muscle weakness associated with type 1 ryanodine receptor T4709M mutation. J.Clin.Invest. US 135 2025 41392983 doi:10.1172/JCI194490 0021-9738 0797 JCINAO EMD-49538 consensus EM volume
Composite map
 EMDB EMD-49538 consensus EM volume
Composite map
 EMDB EMD-73148 associated EM volume Complex of RyR1 with Calstabin-1 (EGTA condition) Complex of RyR1 with Calstabin-1 (EGTA condition) 0 1 2
5 mM EGTA
Mus musculus singleParticle particle 8.5 7.4 10.0 HEPES 400.0 NaCl sodium chloride 0.25 CHAPS 0.01 DOPC 0.5 TCEP 5.0 EGTA ETHANE 100 277.15 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 0.5 1.2 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 BIOQUANTUM (6k x 4k) 1 7612 58.0 1 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION CryoSPARC ab initio 2.6 FSC 0.143 CUT-OFF cryoSPARC 516292 MAXIMUM LIKELIHOOD cryoSPARC
CryoSPARC branch-and-bound
 MAXIMUM LIKELIHOOD cryoSPARC
CryoSPARC branch-and-bound
 emd_73148.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 424.96 424.96 424.96 90.0 90.0 90.0 X Y Z -0.26149008 0.6222946 0.0015123291 0.015191839 0.83 0.83 0.83 0.1 AUTHOR Constituent EM map: Focused refinement on JSol/CSol of mouse RyR1 (including auxiliary transmembrane helix TMx; EGTA-only dataset) emd_73148_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 424.96 424.96 424.96 90.0 90.0 90.0 X Y Z -0.42945307 0.8069383 0.0009665723 0.056043617 0.83 0.83 0.83 AUTHOR Half Map A emd_73148_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 424.96 424.96 424.96 90.0 90.0 90.0 X Y Z -0.40743667 0.79106456 0.0009676768 0.056117676 0.83 0.83 0.83 AUTHOR Half Map B