Locally refined map of the right BCCP in the up conformation of the long chain acyl-CoA carboxylase complex from Mycobacterium smegmatis with ATP, bicarbonate, and propionyl-CoA

2026-04-01 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-10-26 2025-11-26 2025-11-26 2026-04-01 Canadian Institutes of Health Research (CIHR) PJT191893 Canada Locally refined map of the right BCCP in the up conformation of the long chain acyl-CoA carboxylase complex from Mycobacterium smegmatis with ATP, bicarbonate, and propionyl-CoA Liang Y Rubinstein JL Carboxylase, transferase, lipid synthesis, mycolic acid synthesis, LIGASE Liang Y Bueler SA Rubinstein JL Structural basis for substrate specificity and MSMEG_0435-0436 binding by the mycobacterial long-chain acyl-CoA carboxylase complex. Proc.Natl.Acad.Sci.USA US 123 e2530575123 e2530575123 2026 41843674 doi:10.1073/pnas.2530575123 1091-6490 0040 PNASA6 EMDB EMD-73587 associated EM volume Locally refined map of the right BCCP in the up conformation of the long chain acyl-CoA carboxylase complex from Mycobacterium smegmatis with ATP, bicarbonate, and propionyl-CoA Locally refined map of the right BCCP in the up conformation of the long chain acyl-CoA carboxylase complex from Mycobacterium smegmatis with ATP, bicarbonate, and propionyl-CoA 0 1 2 3 4 Mycolicibacterium smegmatis MC2 155 0.87 Biotin-dependent acyl-coenzyme A carboxylase alpha3 subunit AccA3 Mycolicibacterium smegmatis MC2 155 LEVO MANHASSKISKVLVANRGEIAVRVIRAAKDAGLASVAVYAEPDADAPHVRLADEAFALGG QTSAESYLVFEKILDAAEKSGANAIHPGYGFLSENADFAQAVIDAGLIWIGPSPQSIRDL GDKVTARHIAARAKAPLVPGTPDPVKDADEVVAFAKEHGVPVAIKAAFGGGGRGMKVART LEEIPELFESATREAIAAFGRGECFVERYLDKPRHVEAQVIADQHGNVVVAGTRDCSLQR RFQKLVEEAPAPFLTDAQRKEIHESAKRICKEAGYYGAGTVEYLVGQDGLISFLEVNTRL QVEHPVTEETSGIDLVRQQFKIANGEPLDITEDPTPRGHSFEFRINGEDAGRGFLPAPGP VTKFVAPTGPGVRMDSGVETGSVIGGQFDSMLAKLIVTGATREEALERSRRALAEFTVEG LATVIPFHRAVVSDPAFIGDGEKFDVHTRWIETEWNNTVEPFTGGDPIEEEDTVPRQTVV VEVGGRRLEVSLPGDLAIGGGGGAAAPGVVRKKPKPRKRGGGGAKAASGDAVTAPMQGTV VKVAVEEGQEVSAGDLVVVLEAMKMENPVTAHKDGTITGLAVEAGAAITQGTVIAEIK A0QTE1 6.3.4.14 Biotin-dependent acyl-coenzyme A carboxylase beta4 subunit AccD4 Mycolicibacterium smegmatis MC2 155 LEVO MTNKTTAELLAELREKLELAKEPGGEKAVAKREKKGIPSARARINALLDPGSFIEIGALA KTPGDPNALYGDGVVTGRGTIDGRPVGVFSHDQTVFQGSVGEMFGRKVARLMEWVAMVGC PIIGINDSAGARIQDAVTSLAWYAELGRRHEMLRGLVPEISLIFGKCAGGAVYSPIQTDL LVAVRDQGYMFITGPDVIKDVTGEDVTFDELGGADEQAKRGNIHKVVNSEAEAYQYVRDY LSFLPSNHFDNPPIVNPGMEPEITPHDLELDSIVPDADNMAYDMHEILLRIFDDGDVFEI AEQRGPAMITAFARVDGHPVGVIANQPMVLSGAIDNEASDKAASFIRFCDSYNLPLVFVV DTPGAMPGVAEEKGGIIKRGGRFFNAIVEADVPKVTVIIRKAYGGGYAVMGSKQLSADLN FAWPTARIAVIGAEGAAQLLVKRFPDPNAPEVQKIRDDFIEGYNLNMATPWIAAERGYID AVIQPHETRLLLRKSLRLLRDKQNGPKVQRKHGLLPL A0R616 6.4.1.- Biotin-dependent acyl-coenzyme A carboxylase beta5 subunit AccD5 Mycolicibacterium smegmatis MC2 155 LEVO MTSVTEPSAEHQVDIHTTAGKLADLKRRTEETLHPVGEAAVDKVHAKGKLTARERILALL DEGSFVELDALAKHRSTNFGLEKNRPLGDGVITGYGTIDGRDVCIFSQDATVFGGSLGEV YGEKIVKVQELAIKTGRPLIGINDGAGARIQEGVVSLGLYSRIFHNNIKASGVIPQISLI MGAAAGGHVYSPALTDFVVMVDQTSQMFITGPDVIKTVTGEDVTMEELGGAHTHMAKSGT AHYVASGEQDAFDYVRDLLSYLPPNNYADPPLYPVAIPEGSIEETLTDEDLELDTLIPDS PNQPYDMHEVITRILDDDEFLEVQAGYAGNIVVGFGRVEGRPVGIVANQPTQFAGCLDIN ASEKAARFIRTCDCFNIPIVLLVDVPGFLPGTDQEYNGIIRRGAKLLYAYGEATVAKVTV ITRKSYGGAYCVMGSKDMGADVVVAWPTAQIAVMGASGAVGFVYRQQLKEAAKNGEDVDA LRLELQQTYEDTLVNPYIAAERGYVDAVIPPSHTRGYVANALRLLERKIVQMPPKKHGNI PL A0QTE7 6.4.1.3 Biotin-dependent acyl-coenzyme A carboxylase epsilon subunit AccE Mycolicibacterium smegmatis MC2 155 LEVO MSGANDSTTVSGEVQADVTDEAKADHEAHIKVLRGEPTPEEMAALMAVLASAGGGPAEPV KKERNMWGHPVDKLRYSVFSWQRVTLLERTHMRR A0QTE6 singleParticle particle 5 6.0 Homemade COPPER/RHODIUM 400 GOLD HOLEY 35.0 GLOW DISCHARGE 15 AIR ETHANE 90 277 LEICA EM GP TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.1 20.0 130000.0 TFS Selectris X 10 TFS FALCON 4i (4k x 4k) 8000 40.0 1 cryoSPARC 5.0.0-privatebeta PHASE FLIPPING AND AMPLITUDE CORRECTION

Initial model generated ab initio in cryoSPARC v5.0.0-privatebeta

C1 3.2 FSC 0.143 CUT-OFF cryoSPARC 5.0.0-privatebeta 27091 MAXIMUM LIKELIHOOD cryoSPARC 5.0.0-privatebeta MAXIMUM LIKELIHOOD cryoSPARC 5.0.0-privatebeta emd_73587.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 446.4 446.4 446.4 90.0 90.0 90.0 X Y Z -0.41048682 0.7282909 -0.00027095075 0.017032439 0.93 0.93 0.93 0.08 AUTHOR ::::EMDATABANK.org::::EMD-73587:::: Unsharpened map emd_73587_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 446.4 446.4 446.4 90.0 90.0 90.0 X Y Z -0.7551045 1.2278965 -0.00027095075 0.028718706 0.93 0.93 0.93 AUTHOR ::::EMDATABANK.org::::EMD-73587:::: Sharpened map emd_73587_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 446.4 446.4 446.4 90.0 90.0 90.0 X Y Z -1.827182 1.7632041 0.000284608 0.22158061 0.93 0.93 0.93 AUTHOR ::::EMDATABANK.org::::EMD-73587:::: emd_73587_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 446.4 446.4 446.4 90.0 90.0 90.0 X Y Z -1.8582911 1.7729762 0.00028397408 0.22015803 0.93 0.93 0.93 AUTHOR ::::EMDATABANK.org::::EMD-73587::::