2025-12-03 REL RCSB RCSB RCSB 2025-10-29 2025-12-03 2025-12-03 2025-12-03 Chan Zuckerberg Initiative CZII2023327779 United States The Kaggle CryoET Object Identification Challenge: ground truth thyroglobulin Peck A Hutchings J Schwartz J Paraan M thyroglobulin, HORMONE Peck A Hutchings J Schwartz J Yu Y Ermel UH Kandel S Kimanius D Zhao Z Zheng S Artley B List D Silva SA Reade W Asuncion J Evans K Gadling J Khandwala K McCanny S McCarthy DG Ni JX Pourroy J Venkatakrishnan M Wang ZS Agard DA Potter CS Carragher B Harrington K Paraan M Lessons learned from a Kaggle challenge for particle picking in cryo-electron tomography Biorxiv US 2025 doi:10.1101/2025.11.03.686153 2692-8205 Peck A Yu Y Schwartz J Cheng A Ermel UH Hutchings J Kandel S Kimanius D Montabana EA Serwas D Siems H Wang F Zhao Z Zheng S Haury M Agard DA Potter CS Carragher B Harrington K Paraan M A realistic phantom dataset for benchmarking cryo-ET data annotation. Nat Methods US 22 1819 1823 2025 40859020 doi:10.1038/s41592-025-02800-5 1548-7105 EMDB EMD-73642 associated EM volume HEK293T cell lysate mixed with purified proteins on functionalized EM grids HEK293T cell lysate mixed with purified proteins on functionalized EM grids 0 Homo sapiens HEK293T subtomogramAveraging particle 7.5 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.0 2.0 TFS FALCON 4i (4k x 4k) 2.0 1 C2 9.2 FSC 0.143 CUT-OFF RELION 5
Initial low-resolution alignment against the same model. All subsequent high-resolution refinements are independent.
5921 480 5921 RELION 5 RELION 5 PHASE FLIPPING AND AMPLITUDE CORRECTION MAXIMUM LIKELIHOOD RELION 5 emd_73642.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 0 0 0 100 100 100 302.0 302.0 302.0 90.0 90.0 90.0 X Y Z -0.0020935766 0.015479965 0.00033944324 0.0016765777 3.02 3.02 3.02 0.0067 AUTHOR main map emd_73642_msk_1.map emd_73642_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 0 0 0 100 100 100 302.0 302.0 302.0 90.0 90.0 90.0 X Y Z -0.01046394 0.020809822 0.00033750408 0.0022262326 3.02 3.02 3.02 AUTHOR half-map from refinement emd_73642_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 0 0 0 100 100 100 302.0 302.0 302.0 90.0 90.0 90.0 X Y Z -0.009781413 0.021750977 0.00034138237 0.0022257958 3.02 3.02 3.02 AUTHOR half-map from refinement