Helical Reconstruction of the Human Cardiac F-Actin-Tropomyosin Complex

2026-01-14 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-11-20 2026-01-14 2026-01-14 2026-01-14 National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) HL036153 United States National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) HL124091 United States Helical Reconstruction of the Human Cardiac F-Actin-Tropomyosin Complex Karpicheva O Rynkiewicz MJ Lehman W Cammarato A F-actin, tropomyosin, muscle, cryo-EM structure, motor proteins, MOTOR PROTEIN Chitre K Karpicheva OE King CJ Rynkiewicz MJ Fenwick AJ Dawson JF Foster DB Lehman W Cammarato A Pseudo-acetylation of ACTC1 K326 and K328 promotes dysinhibition of reconstituted human cardiac thin filaments. J.Mol.Cell.Cardiol. UK 212 10 15 2025 41443503 doi:10.1016/j.yjmcc.2025.12.008 1095-8584 9zbl FULLOVERLAP EMDB EMD-73993 associated EM volume

Complex of Cardiac F-Actin Containing Mg-ADP with Tropomyosin Complex of Cardiac F-Actin Containing Mg-ADP with Tropomyosin 0 1 2 Homo sapiens Actin, alpha cardiac muscle 1 Homo sapiens 0.042064891 6 Spodoptera frugiperda LEVO MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITN WDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDG VTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEIT ALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF P68032 3.6.4.- Tropomyosin alpha-1 chain Homo sapiens 0.032921773 4 Escherichia coli BL21(DE3) LEVO ASMDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKA TDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDA DRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETR AEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI P09493 ADENOSINE-5'-DIPHOSPHATE 0.000427201 6 ADP MAGNESIUM ION 2.4305e-05 6 MG helical filament 7.0 10.0 C7H15NO4S 3-(N-morpholino)propanesulfonic acid 50.0 CH3COONa Sodium acetate 3.0 MgCl2 Magnesium chloride 1.0 C4H10O2S2 Dithiothreitol Quantifoil R1.2/1.3 COPPER 300 CARBON HOLEY 11.0 GLOW DISCHARGE 45 0.02 ETHANE 100 283 FEI VITROBOT MARK IV

Blotting was performed with a blot force of 3 and a blot time of 3 s..

9 uM F-actin was mixed with 63 uM tropomyosin and incubated for 15 minutes; then 3 uL of the mixture was applied to the grid

TFS GLACIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 50.0 2.7 0.8 2.5 130000.0 OTHER NITROGEN 98.0 98.0 OTHER TFS Selectris cryoSPARC v4.5.3 FEI FALCON IV (4k x 4k) 1 4731 48.96 1 21 27.67 -166.52 C3 2.79 FSC 0.143 CUT-OFF cryoSPARC v4.5.3 1954406 cryoSPARC v4.5.3 PHASE FLIPPING AND AMPLITUDE CORRECTION 2655073 cryoSPARC v4.5.3 Filaments were collected using Filament Tracer Tool emd_73993.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 267.0 267.0 267.0 90.0 90.0 90.0 X Y Z -0.13565306 0.43038404 0.0014374455 0.017669208 0.89 0.89 0.89 0.0796 AUTHOR ::::EMDATABANK.org::::EMD-73993:::: WT Human Actin, Tropomyosin, Cardiac 5jlf PDB experimental model FLEXIBLE FIT MDFF 1.9.4a53 emd_73993_msk_1.map emd_73993_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 267.0 267.0 267.0 90.0 90.0 90.0 X Y Z -0.1570706 0.44318113 0.0005971255 0.023837082 0.89 0.89 0.89 AUTHOR ::::EMDATABANK.org::::EMD-73993:::: emd_73993_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 267.0 267.0 267.0 90.0 90.0 90.0 X Y Z -0.15517886 0.4724183 0.0006043538 0.023824634 0.89 0.89 0.89 AUTHOR ::::EMDATABANK.org::::EMD-73993::::