2026-03-25 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-12-01 2026-03-25 2026-03-25 2026-03-25 Howard Hughes Medical Institute (HHMI) United States National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) R35NS105038 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM140847 United States Human TRPM8 fully-swapped, ligand-free structure in the absence of calcium at 4 degrees Celsius resolved in cell vesicles Choi KY Lin X Cheng Y Julius D TRPM8, transient receptor potential melastatin 8, MEMBRANE PROTEIN, human Choi KY Lin X Cheng Y Julius D Structural energetics of cold sensitivity Nature UK 2026 doi:10.1038/s41586-026-10276-2 1476-4687 0006 NATUAS 9zf0 FULLOVERLAP EMDB EMD-74124 associated EM volume
Human TRPM8 fully-swapped, ligand-free structure in the absence of calcium at 4 degrees Celsius resolved in cell vesicles
 Homotetrameric complex of human TRPM8 determined in cell-derived vesicles. Homotetrameric complex of human TRPM8 determined in cell-derived vesicles. 0 1 Homo sapiens Transient receptor potential cation channel subfamily M member 8 Homo sapiens 0.128334219 4 Homo sapiens LEVO GPGSGSGMSFRAARLSMRNRRNDTLDSTRTLYSSASRSTDLSYSESDLVNFIQANFKKRECVFFTKDSKATENVCKCGYA QSQHMEGTQINQSEKWNYKKHTKEFPTDAFGDIQFETLGKKGKYIRLSCDTDAEILYELLTQHWHLKTPNLVISVTGGAK NFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKYIGEVVRDNTISRSSEENIVAIGIAAWGMVSNRDTLIRNCDAE GYFLAQYLMDDFTRDPLYILDNNHTHLLLVDNGCHGHPTVEAKLRNQLEKYISERTIQDSNYGGKIPIVCFAQGGGKETL KAINTSIKNKIPCVVVEGSGQIADVIASLVEVEDALTSSAVKEKLVRFLPRTVSRLPEEETESWIKWLKEILECSHLLTV IKMEEAGDEIVSNAISYALYKAFSTSEQDKDNWNGQLKLLLEWNQLDLANDEIFTNDRRWESADLQEVMFTALIKDRPKF VRLFLENGLNLRKFLTHDVLTELFSNHFSTLVYRNLQIAKNSYNDALLTFVWKLVANFRRGFRKEDRNGRDEMDIELHDV SPITRHPLQALFIWAILQNKKELSKVIWEQTRGCTLAALGASKLLKTLAKVKNDINAAGESEELANEYETRAVELFTECY SSDEDLAEQLLVYSCEAWGGSNCLELAVEATDQHFIAQPGVQNFLSKQWYGEISRDTKNWKIILCLFIIPLVGCGFVSFR KKPVDKHKKLLWYYVAFFTSPFVVFSWNVVFYIAFLLLFAYVLLMDFHSVPHPPELVLYSLVFVLFCDEVRQWYVNGVNY FTDLWNVMDTLGLFYFIAGIVFRLHSSNKSSLYSGRVIFCLDYIIFTLRLIHIFTVSRNLGPKIIMLQRMLIDVFFFLFL FAVWMVAFGVARQGILRQNEQRWRWIFRSVIYEPYLAMFGQVPSDVDGTTYDFAHCTFTGNESKPLCVELDEHNLPRFPE WITIPLVCIYMLSTNILLVNLLVAMFGYTVGTVQENNDQVWKFQRYFLVQEYCSRLNIPFPFIVFAYFYMVVKKCFKCCC KEKNMESSVCCFKNEDNETLAWEGVMKENYLVKINTKANDTSEEMRHRFRQLDTKLNDLKGLLKEIANKIK Q7Z2W7 singleParticle particle 7.4 300.0 KCl potassium chloride 20.0 C8H18N2O4S HEPES 0.5 C13H17F13NO4P Fos-Choline-8, Fluorinated Quantifoil CARBON CONTINUOUS ETHANE 100 277.15 FEI VITROBOT MARK IV TFS KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 1.0 2.5 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 10 GATAN K3 BIOQUANTUM (6k x 4k) 3 74792 2.0 47.7 1 2552130 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION
Initial model generated ab initio from screening dataset and used for subsequent refinement.
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