Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB composite map

2026-04-08 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.pdbx_database_id_PubMed _citation.title _em_admin.last_update RCSB RCSB 2025-12-12 2026-01-28 2026-01-28 2026-04-08 National Science Foundation (NSF, United States) United States Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB composite map Nissley AJ Cate JHD Ribosome, Hibernation, Archaea Nissley AJ Williams MC Shulgina Y Kivimae RW Nayak DD Cate JHD A family of archaeal hibernation factors that bind in tandem and protect ribosomes in dormant cells. Biorxiv US 2026 41867869 doi:10.64898/2026.01.19.700200 2692-8205 EMD-74841 consensus EM volume EMD-74851 focused EM volume EMD-74853 focused EM volume EMD-74854 focused EM volume 9znf FULLOVERLAP EMDB EMD-74446 associated EM volume

Cryo-EM structure of the Methanosarcina acetivorans 70S ribosome in complex with SriA and SriB

EMDB EMD-74841 consensus EM volume EMDB EMD-74851 focused EM volume EMDB EMD-74853 focused EM volume EMDB EMD-74854 focused EM volume 70S ribosome 70S ribosome 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 Methanosarcina acetivorans 50S subunit 1 1 2 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Methanosarcina acetivorans 30S subunit 1 3 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 Methanosarcina acetivorans 23S rRNA Methanosarcina acetivorans 0.9393629379999999 1 AAUUAGGAAUUAUGCUAUCAGGUGGAUGGCUCGGCUCAAGAGCUUAUGAAGGACGUGCCAAGCUGCGAUAAGCCCGGGGU AGGUGCAUGGAGCCAAUGAACCCGGGAUUUCCGAAUGGGACCUCUCCGUAGUGAUCAUUCAUGAUCGGGAACGCUCCGAA UUGAAACAUCUCAGUAGGAGCGGGAAAAGAAAUCAAUUGAGAUGCCGUAAGUAACGGCGAGUGAAACCGGCACAGUUCAA ACCGAAUCCCUUCGGGGACAUGUGGUGUUGUAGGGCUGUUCAAACGUCUGGCGGUAAAAUCGAACUUGCCUGGAACGGCA GACCAUAGAGUGUGACAGUCACUUAGAUGUACGCCAAAAGACCGGAACAUGUCCCUGAGUACCGUGCGUUGGAUAUCGUG CGGGAAUUUGGGGGGCACCAACCUCCAAAACUAAAUACUCCUUGAGACCGAUAGCGAAAUAGUAGGGUGACCGAACGCUG AAAAGUACCCCGAGAAGGGAGGUGCAAAGUGCCUGAAACCUGAUAGUGAUGGAACGAUACGGCAUGAAAGGAUCUCUGGC AUGAAGGAAUCAUUCGCGAGGAUGUAGUACGAAUGUCACUGCCAGUGUCGUAUCUUACGUUUUGAAGAACGGGCCAGGGA GUGUAUCUUGGUGGCAAUGGCUAACCUUUUAUCUGGGCAGCCGAAGCGAAAGCAACAUGUGCGCAGCCUUUUGGCGAGGC ACGACGUAUACAAGUGCGUGGAGUCACCGGGGUACGACCCGAAGCCGGGUGAUCUAGGCGUGGGCAGGUUGAAGCGUGGC GAAAGCUACGUGGAGGACCGUAAACGGUAUUGAUCUGCAAAUCAUUCGUGUGACCUGCGUCUCGUAGUGAAAUGCUAAUC UAACCCGGCAUCCGCUGGUUCCUUCCGAAACAUGUCGCAGCAUGACCUGACUUGAGAUCGUCGGUGGAGUAGAGCACUGA UUGGCGAUUCCGGGGGAGAAAUCCCUCGCUCGCUUGUCAAACUCCAAACCCCCCGUCAUCCUAGAAAGUCGGAGUCCGGA CUGCUGGGGUAAGCUUGUAGUCCGUAAGGGAGACAACCCAGCCCGUGGUUAAGGUCCCCAAGUGUCGACUAAGUGUUAAU ACUAAAGGGCGUCCCAAGCCCCAGACAGCUGGAAGGUUAGCUUAGAAGCAGCUACCCUUCAAAGAGUGCGUAACAGCUCA CCAGUCGAGGUUUGGGGCCCCGAAAAUUGACGGGGCUCAAGUCGACCACCGAUACCACGGAGUACCGCAAGGUAAUCUCG UAGGAAGGCGUUGUGUUCGGGUCGAAGCAGGGCUGUGAAGUCCUGUGGACCGUUCACAAACGAAAAUCCUGGUAAUAGUA GCAGCAAAGCAGGGUGAGACUCCCUGCCGCCGAAGGGGCCAGGUUUCCUCGGCAAUGUUCGUCAGCCGAGGGUUAGUCGG UCCUAAGACGUACCGUAAUUCGAGUACGCCGAAAGGGAAACAGGUUAAUAUUCCUGUACCAUUUAGCGUUUAGUCUGACG UCUCAGGGCAAGCCGAGCGGCGUCGUCGCGCCGUCUAAGCAGCGAACCCCGUGGAGAGCCGUAAUGGCGAGAAGCGGGCG AAUCUGUAAUGGCACAAGUCGGCUUCACCCCGGGACCCGUGAAAAGGACAGCUAAAUGUCCGUACCGAGAACUGACACAG GUGCCCCUAGCUGAAAAGGCUAAGGCGUGUCGGAGUAAUUCAGUUAAGGGAAUUCGGCAAAUUAGCUCCGUAACUUCGGG AUAAGGAGUGCCUGCUGUGUAGACAGCAGGUCGCAGUGACCAGGGGGCUCUAACUGUCUAAUACCAACAUAGGAGAUUGC AAACCCGUAAGGGCUAGUACAAUCUCUGAAUCCUGCUCAGUGCAGGUACCUGAAACCCCGGUUCAACGGGAAGAAGGGCC UGUAAACAGCGGGGGUAACUAUGACCCUCUUAAGGUAGCGUAGUACCUUGUCGCUUAAUUGGCGACUUGCAUGAAUGGAU CAAUGAGAGCCCUACUGUCCCUAACUGGAGUCCGGUGAAGCUUACAUUCUAGUGCACAGUCUAGAGACCUCUAGGGGGAA GUGAAGACCCCGUGGAGCUUUACUGCAGCCUGUCGUUGGGUUGUGGUUCUGGAUGUACAGUGUAGGUAGGAGGCAUCGAA GCGGGUGCGCCAGCAUUCGUGGAGCCGUCCUUGGGACACUACCCUUCCGGGACUACGACCCUAACUCUGAGAAGAGGACC CCGAUAGGUGGGCAGUUUGCCUGGGGCGGGACGCCCUUGAAAAGAUAUCAAGGGCGCGCAAUGGUUGACUCAAGCGGGUC GGAAACUCGCUGAAGAGUGCAAGAGCAUAAGUCAGCCUGACGUUAUUCAGCACAGCAGUGGAUGACGAGACGAAAGUCGG CUCUAGCGAACUUUUGAGCCUCCUUGGUGGGGGCCAAAAAUGACAGAAAAGUUACCCCGGGGAUAAUUGAGUCGUUGCCG GCAAGAGUACAUAUCGACCCGGCAGCUUGCUAC(5MC)U(5MC)GAUGUCGGUUCUUUCCAUCCUGGCCGUGCAGCAGCG GCCAAGGGUGAGGU(OMU)(OMG)UUCGCCUAUUAAAGGAGAUCGUGAGCUGGGUUUAGACCGUCGUGAGACAGGUCGGU UACUAUCUACUAGAGGUGCCCGAGGUCUGCGGGUAAGCUGCUUUUAGUACGAGAGGAACCAAGCAGCGGCGCCACUGGUG UACCGGUUGUCUGACAAGGCAUCGCCGGGCAGCUACGCGCUCAGGAAUAAGAGCUGAAUGCAUCUAAGCUCGAACUCUGA CCUAAAAAGAGACCUCUUUAAGGAUUCCGGUAAAAGACCGGUUUGAUAGAAACGGGAUGUAAGCACCAAGGCAACGAGGU GUUCAGUCCGCGUUUACUAACCAUUCGUUCCUUUCACUUA NR_076184.1 5S rRNA Methanosarcina acetivorans 0.042646266 1 GAGUUUGGCGGCCAUAGCGGUAGGGCAACUCCUGUACCCAUCCCGAACACAGCAGAUAAGCCUGCCCGCGUUCCUUACUG UACUGAAGUGUGCGAGCCUUCGGGAACUCUGGAUCGCUGCCAAGCUCACCUUA AE010299.1 16S rRNA Methanosarcina acetivorans 0.47845812499999996 1 AUUCUGGUUGAUCCUGCCAGAGGUUACUGCUAUCGGUGUUCGCCUAAGCCAUGCGAGUCAUAUGUUCUUUGUGAACAUGG CGUACUGCUCAGUAACACGUGGAUAACCUGCCCUUGGGUCCGGCAUAACCCCGGGAAACUGGGGAUAAUACCGGAUAACG CACAUAUGCUGGAAUGCUUUCUGCGUAAAACGGAUUCGUCUGCCCAAGGAUGGGUCUGCGGCCUAUCAGGUAGUAGUGGG UGUAACGUACCUACUAGCCGACAACGGGUACGGGUUGUGAGAGCAAGAGCCCGGAGAUGGAUUCUGAGACAUGAAUCCAG GCCCUACGGGGCGCAGCAGGCGCGAAAACUUUACAAUGCGGGAAACCGUGAUAAGGGGACACCGAGUGCCAGCAUCAUAU GCUGGCUGUCCGGAUGUGUAAAAUACAUCUGUUAGCAAGGGCCGGGCAAGACCGGUGCCAGCCGCCGCGGUAACACCGGC GGCCCGAGUGGUGAUCGUGAUUAUUGGGUCUAAAGGGUCCGUAGCCGGUUUGGUCAGUCCUCCGGGAAAUCUGACAGCUC AACUGUUAGGCUUUCGGGGGAUACUGCCAGACUUGGAACCGGGAGAGGUAAGAGGUACUACAGGGGUAGGAGUGAAAUCU UGUAAUCCCUGUGGGACCACCUGUGGCGAAGGCGUCUUACCAGAACGGGUUCGACGGUGAGGGACGAAAGCUGGGGGCAC GAACCGGAUUAGAUACCCGGGUAGUCCCAGCCGUAAACGAUGCUCGCUAGGUGUCAGGCAUGGCGCGACCGUGUCUGGUG CCGCAGGGAAGCCGUGAAGCGAGCCACCUGGGAAGUACGGCCGCAAGGCUGAAACUUAAAGGAAUUGGCGGGGGAGCACA ACAACGGGUGGAGCCUGCGGUUUAAUUGGACUCAACGCCGGACAACUCACCGGGGACGACAGCAAUAUGUAGGCCAGGCC GAAGACCUUGCCUGAAUCGCUGAGAGGAGGUGCAUGGCCGUCGCCAGUUCGUACUGUGAAGCAUCCUGUUAAGUCAGGCA ACGAGCGAGACCCGUGCCCACUGUUACCAGCAUGUCCUCCGGGACGAUGGGUACUCUGUGGGGACCGCCGGUGUUAAAUC GGAGGAAGGUGCGGGCCACGGUAGGUCAGUAUGCCCCGAAUUUCCCGGGCUACACGCGGGCUACAAUGAAUGGGACAAUG GGUCCCUCCCCUGAAAAGGGC(5MU)GGUAAUCUCACAAACCCAUCCGUAGUUCGGAUCGAGGGCUGUAACUCGCCCUCG UGAAGCUGGAAUCCGUAGUAAUCGCGUUUCAAUAUAGCGCGGUGAAUACGUCCCUGCUCCUUGCACACACCGCCCGUCAA ACCACCCGAGUGAGGUAUGGGUGAGGGCACGGACUUCGUGCCGUGUUCGAACCUGAAUUUUGCAAGGGGGGUUAAGUCGU A(6MZ)CAAGGUAGCCGUAGGGG(MA6)(MA6)UCUGCGGCUGGAUCACCUCCU NR_074110.1 CBS domain-containing protein Methanosarcina acetivorans 0.037446184 1 Escherichia coli LEVO MILLNKNTTFVPVEKMNVQPQVNKSGKKAQQKDPHSVSSMGTMRIGPSFKSRIAEHEGKILALATRDVVTLPPTAAIMEA VRIMTERRFRRIPITDAGTGRLEGVVTSVDIIDFLGGGSRNLLVENRFKGNLLAAINEEVRQIMQTDVAYLNDQADFKDA VTTMLERRTGGLPIVNDEMQVIAIFTERNAVELMGGIVTNKTVDEYMTKNVTMVTTDTPIGQAAKVMVQNRFRRLPVVKD GIFAGIVTASDIVHFLGRGDAFSKLTTGNIHEALDQPVGSIVSQELIWTSPGTDMGKAMEIMLEKKIGSLPVLEDGMLRG IITESDFLRGFDLHHHHHH A0A832S9K2 CBS domain-containing protein Methanosarcina acetivorans 0.030268732 1 Escherichia coli LEVO MNVSEIMSEGPVSIKERDFVTHARQLMRDYLFRSLVVVDEGNRLVGMLNDQDIMRVTSTRSNVTVGGYARPSPTVTPDMD VVKAAKLMVQSKQNRVPVVKSTTDHTVVGVLSDVDILRNAELPRSASKTIDMVMTKKVKTCSPDERISKVWNYMTETDYT GIPVVSKKGDPIGMITRRDIIKAGILRMSIEDERAARPNESPKVEKIMSTPAYTLSENDSVKSAIEMIIQHDIGRVTIVN EQGKISGIADRQDLMNAFVNGWSEHHHHHH A0A832SJD6 E-site tRNA Methanosarcina acetivorans 0.024249428 1 GCCCAGGUAGCUCAGUGGGAGAGCGCUGCCCUGAAGAGGCAGUUGUCCCCGGUUCGAAUCCGGGUCUGGGCACCA Large ribosomal subunit protein uL2 Methanosarcina acetivorans 0.025738627 1 LEVO MGKRLISQNRGRGTPTYRAPSHKYKADLRHPRVDENSSLRGEVVGIEHDPARSAPIAKVAFENGEELFLLASEGIAVGNI IECGDDAEVKPGNIVPIGNVPEGFFICNVESKPNDGGKFVRSSGVYATVVTHEATRTAVSMPSGNIKWLNPKCRAVVGIV AGSGRVDRPWLKAGKKYHKMKTRAAKYPRVSAVAMNPRDHPFGGGAWKHPGKPTTVSRNAPPGRKVGLIAARRTGMKR Q8TRU4 Large ribosomal subunit protein uL3 Methanosarcina acetivorans 0.036949644999999996 1 LEVO MASIHRPKRGSLAFSPRKRAKSHIPRFRAWPEATGEPKLQSFAGYKVGMTHVIMVDDTKNSLTQGMEISVPVTVIETPAI RVAAIRAYAEDSTGEKAIAEVWAADLDPELKRRIPIPAAGNQAEALENIGKLIEEGRVSDVRAVIYTLPKSLTGVPKKVP DIMESGISARDLGTKFEYSKTILGTLVSVTDVFKNGTLVDTAAITIGKGTQGPVKRWGIQLMKGKHSRQGSLRQVGTLGA FNPSRVSWRVPQMGQMGYHQRTEFNKRILKIGSDGEEVTPEGGFINYGLVRGDYILIKGSVPGPSKRLIRLRDPIRAKKA DLGEPNILYISRESKQG Q8TRU7 Large ribosomal subunit protein uL4 Methanosarcina acetivorans 0.027516569999999997 1 LEVO MATAKTIDLTGKAVGEVELPAVFDADYRPDLIKKAVLAAQANRLQPYGPRLYSGMETSARGWGSGRGVSHVPRLVNSSRA ARVPHAKGGRRAHPPKPEADRSEKVNTKERRYAIRSAIAATTDPTLVSLRGHIFEAELPIVAVNDLESLERTKQVIEFLE AAGLYEDVLRAKYGRHIRAGRGKLRGRKYKHKKSVLIVAGENTPILKAARNLSGVDVVTVDSLNAELLAPGTHAGRLTVW TESAIGKLEGAFQ Q8TRU6 Large ribosomal subunit protein uL5 Methanosarcina acetivorans 0.018398299 1 LEVO MRTPIVEKVIVHMGVGESGQHLVNAEDILRNITGQEVVRCFAKRTLPAFSIKKNEPIGCKVTLRGQKAQEFLETALGIVE KTLNRSQFDSFGNVSFGIEEHTDFPGMRYDPNIGVFGMDVTVVLKRPGERICKRRIAARKIPAGHRVTVDDAIAFLNESY GVEVM Q8TRT4 Large ribosomal subunit protein uL6 Methanosarcina acetivorans 0.019530715 1 LEVO MVKEIARTIEIPEGVSVSLAQDVFTATGPKGTVERKLWYPGIMIDVKDGEVVVDAEYARKEQKAMVGTFASHIRNLVKGV NEGFECKMSIVYAHFPMQVKVDGKTLIIGNFLGEKKPRFAKIIGETKVKVSGNDVTITGINKEDVGQTAANIEQKTKIKR FDPRIFQDGIYIVQKA Q8TRT1 Large ribosomal subunit protein eL8 Methanosarcina acetivorans 0.012741726 1 LEVO MAQLAKFDVPEELTNKALEALELARDTGKIKKGTNEATKAIERGNAKLVLIAEDIEPAEIVAHIGPLSEEKKAPYIFIKN QKELGAASGLGVSCATVAIVDAGKAAEMVQDIAQKLEALK Q8TQL9 Large ribosomal subunit protein uL13 Methanosarcina acetivorans 0.016085799 1 LEVO MLKMTVIDAKGLILGRLASSVAKQLLSGDEKVYIINAEQAIISGSRAATLREYRETRERGATEFGPYFPKRPDRILKRTI RGMLPYKRARGRDAMSRLKVYVGVPYELKGAETVTIPDADMRLLSSSRYVELGEVSQKMGSKF Q8TT43 Large ribosomal subunit protein uL14 Methanosarcina acetivorans 0.014356906999999999 1 LEVO MKGMRSNIPRALNAGAQIACVDNTGAKVVEIISVKKYRGVKNRMPCAGIGDMCVVSVKKGTPEMRKQVLLAVVVRQKQEF RRPDGLHVSFEDNAMVITDEEGIPKGTDIKGPVAREVAERFPKIGTTASIIV Q8TRT7 Large ribosomal subunit protein uL15 Methanosarcina acetivorans 0.015129008999999999 1 LEVO MDTKKFRGSRTCGGGTHKNRRGAGNRGGRGKAGGCKHHFVRAMMRGYSYGKHGFKRPDEVSRDVSIVNVGELDELASYLV EEGLAEVKDGAYHINLENLGIEKVLGSGRVTKNLVVTSEEFSASAREKIENAGGSCIDAE Q8TRS5 Large ribosomal subunit protein eL15 Methanosarcina acetivorans 0.022650105 1 LEVO MVKSFYGYVRDAWKNPDETYVNELRWERLQVWRKQGSVTRIERPTRIDRARSLGYKAKQGIVVVRVNVRRGGLGHVRPNR GRRTQKMGKNKVSGGMSIQRIAEVRADRRYPNLEVLNSYWVGEDGKHKWFEVILVDPHHPVIKSDKNLNWVCDPSSRGRA TRGKTSAGRKGRGMATRGKGTEKTRPSIRAYKSRGK Q8TPX0 Large ribosomal subunit protein uL16 Methanosarcina acetivorans 0.019409648 1 LEVO MVRKPGSMYRNVRQRSFTRRKYMGGVPGSQVIHYDMGDKANTSFPVKISLIVEEKCQIRHTALEAARITANRHLVADTGK MGFYMKLRVYPHEVIRENKQATGAGADRVSSGMRRAFGKNVGTAARVKPMQKIFTVAVEKQNFKAAKEALWHAGQKLPTP CRIVVDEGAELVQ Q8TU90 Large ribosomal subunit protein uL18 Methanosarcina acetivorans 0.019233713 1 LEVO MATGPRYKVPFRRRREGRTNYHLRLKLLLSRQDRVVVRKSSRNVQIQLIAPTPDGDITYSSAVSNELAKYGYTGATGNTT AAYLTGLLFGLKSLQKGYEGGILDIGLQASSAGSRVYAALKGVVDSGFEIPCSPEVFPPDERIRGEHIAGYREESSDLPE QFEATKEKIFAEFS Q8TRS8 Large ribosomal subunit protein eL18 Methanosarcina acetivorans 0.013714988999999999 1 LEVO MGKKSLVKLTRKTNPRIVSLILTLKERANGDSAPIWKDIAKRLEAPSRNYAAVNISKINRHTAEDDVLLIPGKVLGAGLL DHPVTIAALTFSDSAFEKITEAGGKCLSLEEIMEANPKGSGIRIFR Q8TT44 Large ribosomal subunit protein eL19 Methanosarcina acetivorans 0.017061982 1 LEVO MSDLSNQRKLASKVLGCGLDRVWLNPEASEEIASAITREDIRGLIEEGTIKAKPVKGVSRGRARALAAKRKYGHCKGHGS RKGKKGARTPKKEQWIKKIRALRRRLKELRADGALDKSVYCRLYRKAKGGEYRSVSHLNSHLESEKLLKKE A0A832W9G6 Large ribosomal subunit protein eL20 Methanosarcina acetivorans 0.006972045 1 LEVO MVMMKSFIVKGKFKAGSTWEKFTKKIESQNEKNATDKTYSIFGSKHGVKRSQVQIESVAEE Q8TIN5 Large ribosomal subunit protein eL21 Methanosarcina acetivorans 0.011063719999999999 1 LEVO MTNSHGEKRCTRYKLQKTVRERGISPVSRAIQEFEEGQMVHIDIDPSVQKGMPNPKFQGSTGKVIGQRGRSYVLEVRSGN AMKEVFSLPQHLKPQKY Q8TQU3 Large ribosomal subunit protein uL22 Methanosarcina acetivorans 0.016764578000000002 1 LEVO MARINYSINGDPETTSKAMGSELHISPKKSREVCCKIKGMKASEARKFLEDVIAMKQAVPFKKHHDGSGHRKGPMAAGKY PISASKEILKVLKNAESNAEYKGLEPANMYIIHAAIQRGRVIHGFMPRARGRATPKDTETVNIEMILSEVR Q8TRU2 Large ribosomal subunit protein uL23 Methanosarcina acetivorans 0.009447193999999999 1 LEVO MSSINYPFVTEKAMMLLDENKLQFIVDTRSNKKQILEDVEKMYGFKVKSVRTMTTMKGMKKAVLAFEEPEAAHEIATRIG LM Q8TRU5 Large ribosomal subunit protein uL24 Methanosarcina acetivorans 0.013075340999999999 1 LEVO MIAMVSKQPRKQRKARYAAPLHIRQKFMGARLSEALTKEYGTRSAAVITGDTVKVMRGDFKGTEGKVQSVSLMDGTITVD GVISTKVDGTEVPRPLNPSNVMITKLEMKDGRRASSIKK Q8TRT6 Large ribosomal subunit protein eL24 Methanosarcina acetivorans 0.007204595999999999 1 LEVO MEQRKCYFCGKMLEPGTGKLYVKKDGSTYFMCSSKCMSNFALGRLPRRTEWTEKGKIQLKKA Q8TQL7 Large ribosomal subunit protein uL29 Methanosarcina acetivorans 0.007622636999999999 1 LEVO MAILRTSEIRTMTIEERADELENLNNELVRERALTSAGGAPENPGRIGEIRRTIARIKTIQHELNEI Q8TRU0 Large ribosomal subunit protein uL30 Methanosarcina acetivorans 0.017627416 1 LEVO MYAVVRLRGQVNVRYTIEDTMKMLRLHKVNHCVFVPENPHYKGMVQKVKDYVAYGKIDAKTLAEVLENRGRLEGDTRLTE EYIRENTDYDSIQAFAEAVIEGKSSLKDIPKLKPVFRLHPPRKGHAGIKRTVQQGGVLGNHDENINVLLHKMR Q8TRS6 Large ribosomal subunit protein eL30 Methanosarcina acetivorans 0.010316188 1 LEVO MMKMKINVDKSLIKAVKTGKVIVGANRTIDAAANGSAKMVVLASNCPEDIKKKIQATDIPVLEYEGTSVELGPVCGKPFT IAAMAILDVGESDILAATA Q8TRB9 Large ribosomal subunit protein eL31 Methanosarcina acetivorans 0.010331096 1 LEVO MADDMVKEQIYTIPLREVRKVPAWKRANRAVIEVRGFLVRHMKTESVQVKLDKSINEHLWEKGCEKPPLSIRVRAVKFAD GEVQAELAQ Q8TIN3 Large ribosomal subunit protein eL32 Methanosarcina acetivorans 0.014335579999999999 1 LEVO MDPESRRLFNVRKVQKGKKPQFKRTCSHKFKRLDDNWRRPRGSQGKQRRKYVSKGALVQVGYGSPAAVKGLHPSGYSDVL ISSIAELELVDPSYEAIRIAGTIGAQKKALILAKAEEAGIKVLNSGRSE Q8TRT0 Large ribosomal subunit protein eL37 Methanosarcina acetivorans 0.006456627999999999 1 LEVO MSKGTSSMGKRQKRTHAKCRRCGSVSFNVHTKQCTSCGFGKTSRMRTYKWQAKCKY Q8TL48 Large ribosomal subunit protein eL39 Methanosarcina acetivorans 0.006257515999999999 1 LEVO MSHNMKGQKKRLAKAHKQNTRVPVWVIVKTNRKVVSHPRRRHWRRRSLDVK Q8TIN2 Large ribosomal subunit protein eL40 Methanosarcina acetivorans 0.0056027229999999996 1 LEVO MARFPEAEERLLNKKICMKCNARNAIRATRCRKCGYGTLRVKSKESKGA Q8TJ19 Large ribosomal subunit protein eL42 Methanosarcina acetivorans 0.010872953999999999 1 LEVO MKIPKRFRTYCPFCKTHNEVVVERVKKGQASSMTHIARQKKRQEGIGNSGKFSKVPGGDKPTKRIWLRYRCTVCKKAHQR PCFRAKKFEFKE A0A832W9V9 Large ribosomal subunit protein eL43 Methanosarcina acetivorans 0.010557483 1 LEVO MAKKFTKKGRISRSAGRFGPRYGRKDRKLVADLEERMRAPHVCTKCARPTVERIGTGIWKCSKCGHTFAGGTYIPYTSVG QTLLRTMKNIAEAK A0A832SMV9 Small ribosomal subunit protein eS1 Methanosarcina acetivorans 0.022938651999999997 1 LEVO MARKKVQRKLDGWKSKEWYNIEAPVYLNRAIVGNTMAGDPSLLVGRNVETTVGELTNDMTKNNTKVILRINNVVGDIATT DLMGHELTTDYIRSIVKRQTSRIDANVDVKTKDGYVIRVKPTCFTIKRARSSQMQAIREMMVEIVKKRAAESDFETFMQE AILGRLSAAIYRQAKFIYPLRRVEIRKTEVETIPAAAPAPAAA Q8TKI9 Small ribosomal subunit protein uS2 Methanosarcina acetivorans 0.024807072 1 LEVO MAEAKPALEKEAAVKTGSIPSESEDETASHKEGSTSLVSIDEYLAAGVHIGTQQKTQDMMRFVYRVRTDGLYVLDIQSTD ERIRVASKLLSHYDPARILVVSSRQYGQHPARMFSRALGTRAMLGRFIPGSLTNPQIHGFFEPDVIIVTDPAGDAQVLKE ASSIGVPVVALCDTNNLTSNVDLVIPTNNKGRKALSLVYWLLAREVSRLNDTPFNYELTDFETPL Q8TT39 Small ribosomal subunit protein uS3 Methanosarcina acetivorans 0.034780445 1 LEVO MAIEKKFVNDGYVKASMDEYFAEQLSRAGYGGMELNRTPMGTQIVIYSEKPGMVIGKAGKVIRKLTRDVANKYSLENPQI DAQEVKRPELNAQMMASRLAASIERGWYFRKAGHNTLRAVMNAGALGCEVVISGKLTGARSRVEKFVDGYIKHSGNPVEE VVDEGFAVAVKKLGTLGCKVRIIQPGVVLPDSYKVRESVEVEEPAEKPAEKPAEKPAEKAAAPKKEAAKARAPAPAPEAP APAPEAPAPAPVEEAEVAEPEEAEEVQAETSEEIEGAELVYVEGSDEVRRQVNGVWQHKHGSYDYWHPMARVHKEAKE Q8TRU1 Small ribosomal subunit protein uS4 Methanosarcina acetivorans 0.024559276999999997 1 LEVO MAYPGKKSKSYETPKHPWQEARMATEVQLVKAYGLRNKKEVWKAASMLRMYRSEARKLLAHVASGQEGGLEGHYRTQSEE ILSKLIRYGIIKSDANIDDILSLKTENILERRLQTQVLRLGLARTVIQARQFITHGHIAINGRKATIPGMLVSKEDEMHI GYYGNSPLKNESHPERPVQVASFLADSGTTLKAAAEAKQAREKPPERGGGRRKRGGRR Q8TRR1 Small ribosomal subunit protein eS4 Methanosarcina acetivorans 0.026301408 1 LEVO MTHQKRLSVPKSWKVGKKGNKWISTTRPGPHSQARSLPLGIIIRDILKLVDNSREGKRILSEGKVLVDGIPRKDLRFPVG LFDVITLPLVNEAYRMLQDEKGRLTLHKLNETNVNKLCRINNKTTVKGGKFQLNLNDGTNIIGSNEYGTKDSLILSIPDK QVVKHLKFEVGNLAMVVGGQHSGETGKIMEIREVKSSRHNTVMISGETDFETIEDYVIVIGEDKPEIRLGGEISE Q8TRT5 Small ribosomal subunit protein uS5 Methanosarcina acetivorans 0.022732264 1 LEVO MAFDQDWVPKTRLGKLVVEGQVASMDEAIKSGLPIREPQIIDMLLPDLEDEVLDINMVQRMTDSGRRVKFRATVIVGNRN GYVGLGQAKDVQVGPAIRKAIDAAKLNITYIRRGCGSWECACGLPHTVPYEVTGKAGSVSVTLIPAPRGLGIAAGNTATK VLEKAGIKDVWTKTFGTTRSTLNFAKATYDALNQVNVVRLPVYYGKEEV Q8TRS7 Small ribosomal subunit protein eS6 Methanosarcina acetivorans 0.014474798 1 LEVO MANFKVVVSDPKEGRAYQIDIKDAEANALIGKAIGDVVDGAIFGLAGYKVQITGGCDGSGFVMKPDLPGPRRQRILTATG VGYVPKLPGQRRRKMMRGKEIAPDIIQVNAKIVEYGSKSIKALLGLETAEEAPAAE Q8TQL4 Small ribosomal subunit protein uS7 Methanosarcina acetivorans 0.021062349999999997 1 LEVO MIFLYKIFGKWDPTEVEVKDLGIKRYVSLTPVIVPHSSGKHARQQFNKSEISIVERLANNLMRTETNTGKKQVTLRAVEE AFDIVNKKTKQNPIQVLVDAIANAGPREEVVRLKYGGISVPKAVDTAPQRRVDTALRYISMGTNAAAFKSKRSVAECLAT ELIGAANRDTKSFSINRKDAKERVAKAAR Q8TRC2 Small ribosomal subunit protein uS8 Methanosarcina acetivorans 0.014085300999999998 1 LEVO MVLLDPLANALSTIKNAEAIGKSSCVVRPASKNIGNVLKVMQDLGYIGDFEFIDDGKAGIYSVTLVGRINKCGAIKPRYS VGTASFERWEKQFLPAKNFGALIVTTSSGVMSQYEARDKKIGGQLLAYVY Q8TRT2 Small ribosomal subunit protein uS9 Methanosarcina acetivorans 0.014512665999999999 1 LEVO MVKVVNSSGKHKTATARATVMKGTGKVRINKIPLELYTPELAMMKISEPLLIAGNDVVSGLDINVDVRGGGIIGQANAVR TAVARGIVEWTNDTAIRDNFAAYDRNLLVSDSRQKESKNFGGPGARAKYQKSYR Q8TT42 Small ribosomal subunit protein eS8 Methanosarcina acetivorans 0.013801993 1 LEVO MRWQGSSRRKATGGKVITARGKRKFEMGRESAETRISEIKRKKVPTMGGNRKVRLLQSNVANVTNPKDGKTVTAPIETVI DNTANKHYVRRNILTKGSVIRTSMGTARVTSRPGQDGVVNAVLIE Q8TS82 Small ribosomal subunit protein uS10 Methanosarcina acetivorans 0.01138028 1 LEVO MQKARIRLSGISPKDLDGVCNQVKSIAERTGVNISGPVPLPTKKLVVPTRKSPSGDGTATWDHWEMRVHKRLIDIAADER ALRQLMRIQVPKDINIEIVLEG P61929 Small ribosomal subunit protein uS11 Methanosarcina acetivorans 0.013324279 1 LEVO MADMKWAVAHIKSSFNNTIITVTDITGAETIAKSSGGMVVKAARDESSPYTAMQMAGQLADQLKDKGINGIHIRVRAPGG NKQRSPGPGAQAAIRAFARAGIRIGRIEDVTPVPH(IAS)GTRPKGGRRV Q8TRR0 Small ribosomal subunit protein uS12 Methanosarcina acetivorans 0.01554014 1 LEVO MAKGKYAANILKQTRKDARWKDTYYGRRVLGLNVKADPLGGAPQGRGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGRQVT AFCPGDGAVNFIDEHDEVTVEKIGGRMGGAMGDIPGVRFKVIAVNNVSLNQLVIGRMEKPRR Q8TRC1 Small ribosomal subunit protein uS13 Methanosarcina acetivorans 0.018315210999999998 1 LEVO MLYALQWRKYMVEENNNEELRHLVRIMNTDLQGAKPVQYALTGLPGVGRRTAKLIAKGAGVDPSAILGYLPEEEVAKLDT AIGKFEDIVPSWMLNRQKDLATGQDKHLLGTDILLTFREDINNLKKVRAYRGLRHERGLKVRGQRTKSTGRRGSTVGVSR KK Q8TRR2 Small ribosomal subunit protein uS14 Methanosarcina acetivorans 0.005971020999999999 1 LEVO MTETIKKSGRGVNECKRCGRKQGLVRKYDIYLCRHCFREIAHEMGFEKYS Q8TRT3 Small ribosomal subunit protein uS15 Methanosarcina acetivorans 0.017503594 1 LEVO MAKMHTKRKGKSSSTRPIRTDPPEWCKIGADEVTTIVLDLWKQGVSTAEIGMVLRDRYGVPDAKLITGKKVTTILKENNV APNIPEDLTNLIVKALGLRKHLSVNKKDVHNKRSLNLTESKIRRLVKYYKQEKVLPRDWFYKPETAEMMITR Q8TS56 Small ribosomal subunit protein uS17A Methanosarcina acetivorans 0.012184246 1 LEVO MARDIGLNIPAPSEECDDAYCPFHGTLPVRGQILVGTVVSSKMDNTVVIERQYMKMVSKYQRYEKRRSKIHAHNPACISA KVGDIVTIAECRPISKTKSYVVVKAEVPK Q8TRT8 Small ribosomal subunit protein eS17 Methanosarcina acetivorans 0.007477688 1 LEVO MGNIRETNIKRTAFSLLENYGDVFTKDFETNKALVTKYTTIESKIIRNRVAGYVTRKVARMKVY Q8THP2 Small ribosomal subunit protein uS19 Methanosarcina acetivorans 0.015467917 1 LEVO MAKKSSSRLPKRKGEYTYRGKTVSELQELSLEEFAELLPSRERRSLKRGFTDGQKKVLHEFKEGKKIRTHHRDMIILPEM IGKTIEIHNGKGFVSVDLQPEMVGHRFGEFAPTRSRVSHGSAGVGATRSSKFVPLK Q8TRU3 Small ribosomal subunit protein eS19 Methanosarcina acetivorans 0.016563168 1 LEVO MTTVFDVPATEMIEKLAGILKEDDKIVPPEWAGNVKTGVHKELPPTNDDWWYIRCAAVLRKIYTDGPIGIERLRSVYGGK KDNGSKPFHKAKGSGSVARKTVQQLEAAGFLQKVKDGRTVSAKGRSMMDNAAHELKLELLEKIPELAKY A0A832W9Q0 Putative zinc finger domain-containing protein Methanosarcina acetivorans 0.00616226 1 LEVO MSSQKVEYCTSCGIRLVEKGYVKFPCPQCGAEIGRCISCRQQGNVYTCPKCGFKAP A0A832S8D7 Small ribosomal subunit protein eS24 Methanosarcina acetivorans 0.011620263 1 LEVO MDIKILKDKNNSLLNRRELDFVVKYEGSTPSRSDVRNKLAAMLNAPLELLIIQRIKTEYGMQESKGYAKVYADEARMKQV EQEYILKRNPAPGAEAEEEEA Q8TJT2 Small ribosomal subunit protein eS27 Methanosarcina acetivorans 0.006955049 1 LEVO MVDYTQRPKSRFLRVKCNDCENEQIIFGSASRKITCVVCGRTLAEPTGGKSTITTHILEVLE P61931 Small ribosomal subunit protein eS28 Methanosarcina acetivorans 0.008268642999999999 1 LEVO MLIMAEESTIGGFAAEVIDVIGNTGMHGEASQIQCRVLEGRDKGRVITRNCVGPVRIGDILMLLETSREAKKLTTR Q8TQL8 DUF5350 family protein Methanosarcina acetivorans 0.0064347070000000004 1 LEVO MGKTGSIDWVKVKGRKGRVIKIQKSKSQKAHPGPAQRFTSSGHKRRFIRRSAKALVK A0A832SMB7 MAGNESIUM ION 2.4305e-05 305 MG ZINC ION 6.5409e-05 11 ZN water 1.8015e-05 8740 HOH singleParticle particle 0.42 7.5 25.0 C8H18N2O4S HEPES 50.0 KCl potassium chloride 10.0 MgCl2 Magnesium chloride 2.0 C4H10O2S2 DTT UltrAuFoil R1.2/1.3 GOLD 300 CARBON CONTINUOUS GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 0.5 1.5 105000.0 SerialEM GATAN K3 (6k x 4k) 1 9090 40.0 1 2488798 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION 2.03 FSC 0.143 CUT-OFF cryoSPARC 95430 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC cryoSPARC emd_74446.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 423.1168 423.1168 423.1168 90.0 90.0 90.0 X Y Z -0.14611177 0.8560852 -0.000000000111351 0.03869236 0.8264 0.8264 0.8264 0.138 AUTHOR ::::EMDATABANK.org::::EMD-74446:::: Composite map for the M. acetivorans 70S ribosome in complex with SriA and SriB Other other

ModelAngelo