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    <admin>
        <current_status>
            <date>2026-01-28</date>
            <code>REL</code>
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        </current_status>
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        </revision_history>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-12-23</deposition>
            <header_release>2026-01-28</header_release>
            <map_release>2026-01-28</map_release>
            <update>2026-01-28</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</funding_body>
                <code>R01- AI179697</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>HIV-1 ADA.CM Env</title>
        <authors_list>
            <author ORCID="0000-0002-8964-3699">Croft JT</author>
            <author ORCID="0000-0001-5577-9873">Lee KK</author>
        </authors_list>
        <keywords>HIV-1, Env, membrane, fusion, VIRAL PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0000-0002-8964-3699" order="1">Croft JT</author>
                    <author ORCID="0000-0002-6497-4096" order="2">Do HN</author>
                    <author order="3">Leaman DP</author>
                    <author order="4">Lovendahl KN</author>
                    <author order="5">Ralli-Jain P</author>
                    <author order="6">Chase KJ</author>
                    <author order="7">Chen C</author>
                    <author order="8">Prasad VM</author>
                    <author ORCID="0000-0002-1220-513X" order="9">Derdeyn CA</author>
                    <author ORCID="0000-0002-0885-2502" order="10">Zwick MB</author>
                    <author ORCID="0000-0002-9368-3044" order="11">Gnanakaran S</author>
                    <author ORCID="0000-0001-5577-9873" order="12">Lee KK</author>
                    <title>Structure of HIV-1 Env glycoprotein on virions reveals an alternative fusion subunit organization and native membrane coupling.</title>
                    <journal_abbreviation>Biorxiv</journal_abbreviation>
                    <country>US</country>
                    <year>2026</year>
                    <external_references type="PUBMED">41542432</external_references>
                    <external_references type="DOI">doi:10.64898/2026.01.09.698652</external_references>
                    <external_references type="ISSN">2692-8205</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <accession_id>EMD-74789</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
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        <name>HIV-1 06TG.HT008</name>
        <supramolecule_list>
            <virus_supramolecule supramolecule_id="1">
                <name>HIV-1 06TG.HT008</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <sci_species_name ncbi="587638">HIV-1 06TG.HT008</sci_species_name>
                <natural_host database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_host>
                <virus_type>VIRUS-LIKE PARTICLE</virus_type>
                <virus_isolate>STRAIN</virus_isolate>
                <virus_enveloped>true</virus_enveloped>
                <virus_empty>false</virus_empty>
            </virus_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>HIV-1 ADA.CM Env</name>
                <natural_source database="NCBI">
                    <organism ncbi="587638">HIV-1 06TG.HT008</organism>
                </natural_source>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="9606">Homo sapiens</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLRNVTNSSSEGMRGEIKNCSFNITTSIRDKVKKDYALFYRLDVVPIDNDNTSYRLINCNTSTITQACPKVSFEPIPIHYCTPAGFAILKCKDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSSNFTDNAKNIIVQLKESVEINCTRPNNNTRKSIHIGPGRAFYTTGEIIGDIRQAHCNISRTKWNNTLNQIATKLKEQFGNNKTIVFNQSSGGDPEIVMHSFNCGGEFFYCNSTQLFNSTWNFNGTWNLTQSNGTEGNDTITLPCRIKQIINMWQEVGKAMYAPPIRGQIRCSSNITGLILTRDGGTNSSGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGTIGAMFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIRQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNNMTWMEWDREINNYTSLIHSLIEEAQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGFLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELKNSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL</string>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
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                    </vitrification>
                </subtomogram_averaging_preparation>
            </specimen_preparation_list>
            <microscopy_list>
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                    <microscope>TFS GLACIOS</microscope>
                    <illumination_mode>OTHER</illumination_mode>
                    <imaging_mode>OTHER</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
                    <nominal_defocus_min units="µm">2.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">5.0</nominal_defocus_max>
                    <nominal_magnification>22000.0</nominal_magnification>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">2.4</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
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            </microscopy_list>
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                    <applied_symmetry>
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                            <name>EMAN2</name>
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                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
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                    <type>PROJECTION MATCHING</type>
                    <projection_matching_processing/>
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        <label>::::EMDATABANK.org::::EMD-74789::::</label>
        <annotation_details>HIV-1 ADA.CM Env (Immature VLPs)</annotation_details>
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                    <slow>Z</slow>
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                <annotation_details>HIV-1 ADA.CM Env (Immature VLPs) half map 2</annotation_details>
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