2026-02-04 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2026-01-25 2026-02-04 2026-02-04 2026-02-04 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM114611 United States The G. Harold and Leila Y. Mathers Foundation United States Chan Zuckerberg Initiative United States Cryo-EM structure of human UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) in complex with APPB, Chain B map Kirsh JM Ochoa JM Soroush-Pejrimovsky MT Kaudeer BY Clemons WM polyprenyl-phosphate N-acetylhexosamine phosphate transferase, phosphoglycosyltransferase, membrane protein, TRANSFERASE Kaudeer BY Kirsh JM Mitachi K Ochoa JM Soroush-Pejrimovsky MT Li YE Nguyen VN Kurosu M Clemons Jr WM Structures of bacterial and human phosphoglycosyltransferases bound to a common inhibitor inform selective therapeutics. Biorxiv US 2025 41446129 doi:10.64898/2025.12.16.694696 2692-8205 EMDB EMD-75259 associated EM volume Human UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) in complex with APPB Human UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) in complex with APPB 0 1
DPAGT1 incubated with small molecule APPB for one hour on ice
Homo sapiens 0.100312 UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Homo sapiens
Expressed as fusion protein with C-terminal TEV cleavage site followed by GFP; GFP was cleaved using TEV protease
Homo sapiens LEVO MWAFSELPMPLLINLIVSLLGFVATVTLIPAFRGHFIAARLCGQDLNKTSRQQIPESQGVISGAVFLIILFCFIPFPFLN CFVKEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVVPKPFRPILG LHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGDCRDDHVFSLYFMIPFFFTTLGLLY HNWYPSRVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFMPQVFNFLYSLPQLLHIIPCPRHRIPRLNIKTGKLEMSYSK FKTKSLSFLGTFILKVAESLQLVTVHQSETEDGEFTECNNMTLINLLLKVLGPIHERNLTLLLLLLQILGSAITFSIRYQ LVRLFYDVGSSASENLYFQ 2.7.8.15 singleParticle particle 4.0 7.5
50 mM HEPES pH 7.5, 200 mM NaCl, 5 mM MgCl2, 0.022% (w/v) DDM, and 2 mM TCEP
 ETHANE 100 277 FEI VITROBOT MARK IV
2-3 uL of sample distributed on grid with blot force +8 and blot time 3 seconds.
DPAGT1 incubated with 0.4 mM APPB on ice for 1 hour
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 3.0 NITROGEN 20 GATAN K3 (6k x 4k) 1 70.0 1 cryoSPARC 4.7.1 PHASE FLIPPING AND AMPLITUDE CORRECTION AlphaFold2 prediction of dimeric apo DPAGT1 3.02 FSC 0.143 CUT-OFF cryoSPARC 4.7.1 82209 MAXIMUM LIKELIHOOD cryoSPARC 4.7.1 MAXIMUM LIKELIHOOD cryoSPARC 4.7.1 emd_75259.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 312.0 312.0 312.0 90.0 90.0 90.0 X Y Z -0.38401592 0.63463753 0.00025529042 0.010707463 0.65 0.65 0.65 0.0273 AUTHOR Sharpened Map for Local Refinement of Chain B RIGID BODY FIT REAL 79.700000000000003 emd_75259_msk_1.map emd_75259_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 312.0 312.0 312.0 90.0 90.0 90.0 X Y Z -0.3800382 0.37272382 0.0002276016 0.044656053 0.65 0.65 0.65 AUTHOR Chain B Map Half Map 1 emd_75259_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 312.0 312.0 312.0 90.0 90.0 90.0 X Y Z -0.39135668 0.4089674 0.00022481338 0.044578515 0.65 0.65 0.65 AUTHOR Chain B Map Half Map 2