2026-03-25 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2026-01-29 2026-03-25 2026-03-25 2026-03-25 National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R37 AI150453 United States National Institutes of Health/National Cancer Institute (NIH/NCI) R01 AI190198 United States Buth SA Melikian GB IFITM3, transmembrane protein, viral restriction factor, IAV, ANTIVIRAL PROTEIN Buth SA Melikian GB To Be Published 0353 EMD-75342 other EM volume EMD-75343 other EM volume EMD-75344 other EM volume EMDB EMD-75342 other EM volume EMDB EMD-75343 other EM volume EMDB EMD-75344 other EM volume EMDB EMD-75345 associated EM volume A549 cell A549 cell 0

A549 cell expressing IFITM3 transmembrane protein infected with influenza A virus at MOI 1.5

Homo sapiens lung tomography cell 7.2

DMEM media + 10% FSB

POSITIVE Uranyl Acetate

0.1% UA in FS medium

HM20 EMS Formvar Carbon COPPER 200 CARBON CONTINUOUS

IAV infected cells were high pressure frozen, freeze-substituted and stained with 0.1% uranyl acetate, infiltrated with HM20 resin, and polymerized by UV Blocks were sectioned at 200-250nm.

BAL-TEC HPM 010

The sapphire disc containing cell monolayer was sandwiched between two type B HPF planchettes and frozen according to manufacturer's procedure. Hexadecene-1 was used as a filler..

Hexadecene-1 Leica EM UC6 293 250 JEOL 2200FS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 60.0 2.7 8.0 8.0 10000.0 JEOL DIRECT ELECTRON DE-20 (5k x 3k) OTHER 1.0 13.0 1

Dark subtracted and gain normalized, frame alignment and summing using SerialEM on-the-fly implementation

eTomo 242 NONE